Job ID = 14167913 SRX = SRX7848958 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:41 47501762 reads; of these: 47501762 (100.00%) were unpaired; of these: 2604675 (5.48%) aligned 0 times 31887407 (67.13%) aligned exactly 1 time 13009680 (27.39%) aligned >1 times 94.52% overall alignment rate Time searching: 00:13:41 Overall time: 00:13:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17339004 / 44897087 = 0.3862 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:03:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:03:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:03:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:03:50: 1000000 INFO @ Fri, 10 Dec 2021 14:03:54: 2000000 INFO @ Fri, 10 Dec 2021 14:03:59: 3000000 INFO @ Fri, 10 Dec 2021 14:04:03: 4000000 INFO @ Fri, 10 Dec 2021 14:04:07: 5000000 INFO @ Fri, 10 Dec 2021 14:04:12: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:04:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:04:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:04:17: 7000000 INFO @ Fri, 10 Dec 2021 14:04:21: 1000000 INFO @ Fri, 10 Dec 2021 14:04:21: 8000000 INFO @ Fri, 10 Dec 2021 14:04:26: 9000000 INFO @ Fri, 10 Dec 2021 14:04:26: 2000000 INFO @ Fri, 10 Dec 2021 14:04:31: 10000000 INFO @ Fri, 10 Dec 2021 14:04:32: 3000000 INFO @ Fri, 10 Dec 2021 14:04:36: 11000000 INFO @ Fri, 10 Dec 2021 14:04:37: 4000000 INFO @ Fri, 10 Dec 2021 14:04:40: 12000000 INFO @ Fri, 10 Dec 2021 14:04:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:04:45: 13000000 INFO @ Fri, 10 Dec 2021 14:04:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:04:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:04:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:04:48: 6000000 INFO @ Fri, 10 Dec 2021 14:04:50: 14000000 INFO @ Fri, 10 Dec 2021 14:04:50: 1000000 INFO @ Fri, 10 Dec 2021 14:04:54: 7000000 INFO @ Fri, 10 Dec 2021 14:04:55: 15000000 INFO @ Fri, 10 Dec 2021 14:04:55: 2000000 INFO @ Fri, 10 Dec 2021 14:05:00: 8000000 INFO @ Fri, 10 Dec 2021 14:05:00: 16000000 INFO @ Fri, 10 Dec 2021 14:05:00: 3000000 INFO @ Fri, 10 Dec 2021 14:05:04: 17000000 INFO @ Fri, 10 Dec 2021 14:05:05: 4000000 INFO @ Fri, 10 Dec 2021 14:05:05: 9000000 INFO @ Fri, 10 Dec 2021 14:05:09: 18000000 INFO @ Fri, 10 Dec 2021 14:05:09: 5000000 INFO @ Fri, 10 Dec 2021 14:05:11: 10000000 INFO @ Fri, 10 Dec 2021 14:05:14: 19000000 INFO @ Fri, 10 Dec 2021 14:05:14: 6000000 INFO @ Fri, 10 Dec 2021 14:05:17: 11000000 INFO @ Fri, 10 Dec 2021 14:05:19: 20000000 INFO @ Fri, 10 Dec 2021 14:05:19: 7000000 INFO @ Fri, 10 Dec 2021 14:05:22: 12000000 INFO @ Fri, 10 Dec 2021 14:05:24: 21000000 INFO @ Fri, 10 Dec 2021 14:05:24: 8000000 INFO @ Fri, 10 Dec 2021 14:05:28: 13000000 INFO @ Fri, 10 Dec 2021 14:05:29: 22000000 INFO @ Fri, 10 Dec 2021 14:05:29: 9000000 INFO @ Fri, 10 Dec 2021 14:05:34: 14000000 INFO @ Fri, 10 Dec 2021 14:05:34: 23000000 INFO @ Fri, 10 Dec 2021 14:05:34: 10000000 INFO @ Fri, 10 Dec 2021 14:05:39: 11000000 INFO @ Fri, 10 Dec 2021 14:05:39: 24000000 INFO @ Fri, 10 Dec 2021 14:05:39: 15000000 INFO @ Fri, 10 Dec 2021 14:05:43: 12000000 INFO @ Fri, 10 Dec 2021 14:05:44: 25000000 INFO @ Fri, 10 Dec 2021 14:05:45: 16000000 INFO @ Fri, 10 Dec 2021 14:05:48: 13000000 INFO @ Fri, 10 Dec 2021 14:05:48: 26000000 INFO @ Fri, 10 Dec 2021 14:05:51: 17000000 INFO @ Fri, 10 Dec 2021 14:05:53: 14000000 INFO @ Fri, 10 Dec 2021 14:05:53: 27000000 INFO @ Fri, 10 Dec 2021 14:05:56: 18000000 INFO @ Fri, 10 Dec 2021 14:05:56: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 14:05:56: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 14:05:56: #1 total tags in treatment: 27558083 INFO @ Fri, 10 Dec 2021 14:05:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:05:57: #1 tags after filtering in treatment: 27558028 INFO @ Fri, 10 Dec 2021 14:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:05:57: #1 finished! INFO @ Fri, 10 Dec 2021 14:05:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:05:58: 15000000 INFO @ Fri, 10 Dec 2021 14:05:59: #2 number of paired peaks: 1124 INFO @ Fri, 10 Dec 2021 14:05:59: start model_add_line... INFO @ Fri, 10 Dec 2021 14:05:59: start X-correlation... INFO @ Fri, 10 Dec 2021 14:05:59: end of X-cor INFO @ Fri, 10 Dec 2021 14:05:59: #2 finished! INFO @ Fri, 10 Dec 2021 14:05:59: #2 predicted fragment length is 178 bps INFO @ Fri, 10 Dec 2021 14:05:59: #2 alternative fragment length(s) may be 4,178 bps INFO @ Fri, 10 Dec 2021 14:05:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05_model.r INFO @ Fri, 10 Dec 2021 14:05:59: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:05:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:06:02: 19000000 INFO @ Fri, 10 Dec 2021 14:06:03: 16000000 INFO @ Fri, 10 Dec 2021 14:06:08: 17000000 INFO @ Fri, 10 Dec 2021 14:06:08: 20000000 INFO @ Fri, 10 Dec 2021 14:06:12: 18000000 INFO @ Fri, 10 Dec 2021 14:06:13: 21000000 INFO @ Fri, 10 Dec 2021 14:06:17: 19000000 INFO @ Fri, 10 Dec 2021 14:06:19: 22000000 INFO @ Fri, 10 Dec 2021 14:06:22: 20000000 INFO @ Fri, 10 Dec 2021 14:06:24: 23000000 INFO @ Fri, 10 Dec 2021 14:06:27: 21000000 INFO @ Fri, 10 Dec 2021 14:06:30: 24000000 INFO @ Fri, 10 Dec 2021 14:06:31: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 14:06:36: 25000000 INFO @ Fri, 10 Dec 2021 14:06:36: 23000000 INFO @ Fri, 10 Dec 2021 14:06:41: 24000000 INFO @ Fri, 10 Dec 2021 14:06:41: 26000000 INFO @ Fri, 10 Dec 2021 14:06:46: 25000000 INFO @ Fri, 10 Dec 2021 14:06:47: 27000000 INFO @ Fri, 10 Dec 2021 14:06:50: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 14:06:50: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 14:06:50: #1 total tags in treatment: 27558083 INFO @ Fri, 10 Dec 2021 14:06:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:06:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:06:51: 26000000 INFO @ Fri, 10 Dec 2021 14:06:51: #1 tags after filtering in treatment: 27558028 INFO @ Fri, 10 Dec 2021 14:06:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:06:51: #1 finished! INFO @ Fri, 10 Dec 2021 14:06:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:06:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:06:53: #2 number of paired peaks: 1124 INFO @ Fri, 10 Dec 2021 14:06:53: start model_add_line... INFO @ Fri, 10 Dec 2021 14:06:53: start X-correlation... INFO @ Fri, 10 Dec 2021 14:06:53: end of X-cor INFO @ Fri, 10 Dec 2021 14:06:53: #2 finished! INFO @ Fri, 10 Dec 2021 14:06:53: #2 predicted fragment length is 178 bps INFO @ Fri, 10 Dec 2021 14:06:53: #2 alternative fragment length(s) may be 4,178 bps INFO @ Fri, 10 Dec 2021 14:06:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10_model.r INFO @ Fri, 10 Dec 2021 14:06:53: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:06:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:06:55: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:06:55: 27000000 INFO @ Fri, 10 Dec 2021 14:06:58: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 14:06:58: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 14:06:58: #1 total tags in treatment: 27558083 INFO @ Fri, 10 Dec 2021 14:06:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:06:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:06:59: #1 tags after filtering in treatment: 27558028 INFO @ Fri, 10 Dec 2021 14:06:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:06:59: #1 finished! INFO @ Fri, 10 Dec 2021 14:06:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:06:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:07:01: #2 number of paired peaks: 1124 INFO @ Fri, 10 Dec 2021 14:07:01: start model_add_line... INFO @ Fri, 10 Dec 2021 14:07:01: start X-correlation... INFO @ Fri, 10 Dec 2021 14:07:01: end of X-cor INFO @ Fri, 10 Dec 2021 14:07:01: #2 finished! INFO @ Fri, 10 Dec 2021 14:07:01: #2 predicted fragment length is 178 bps INFO @ Fri, 10 Dec 2021 14:07:01: #2 alternative fragment length(s) may be 4,178 bps INFO @ Fri, 10 Dec 2021 14:07:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20_model.r INFO @ Fri, 10 Dec 2021 14:07:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:07:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:07:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05_peaks.xls INFO @ Fri, 10 Dec 2021 14:07:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:07:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.05_summits.bed INFO @ Fri, 10 Dec 2021 14:07:20: Done! pass1 - making usageList (616 chroms): 2 millis pass2 - checking and writing primary data (7669 records, 4 fields): 22 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 14:07:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:07:58: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:08:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10_peaks.xls INFO @ Fri, 10 Dec 2021 14:08:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:08:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.10_summits.bed INFO @ Fri, 10 Dec 2021 14:08:13: Done! pass1 - making usageList (557 chroms): 1 millis pass2 - checking and writing primary data (5420 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:08:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20_peaks.xls INFO @ Fri, 10 Dec 2021 14:08:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:08:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7848958/SRX7848958.20_summits.bed INFO @ Fri, 10 Dec 2021 14:08:23: Done! pass1 - making usageList (449 chroms): 2 millis pass2 - checking and writing primary data (3318 records, 4 fields): 15 millis CompletedMACS2peakCalling