Job ID = 6627126 SRX = SRX7780066 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:51 29990947 reads; of these: 29990947 (100.00%) were unpaired; of these: 2481718 (8.27%) aligned 0 times 20192125 (67.33%) aligned exactly 1 time 7317104 (24.40%) aligned >1 times 91.73% overall alignment rate Time searching: 00:07:51 Overall time: 00:07:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7175381 / 27509229 = 0.2608 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:19:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:19:22: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:19:22: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:19:27: 1000000 INFO @ Tue, 14 Jul 2020 10:19:33: 2000000 INFO @ Tue, 14 Jul 2020 10:19:40: 3000000 INFO @ Tue, 14 Jul 2020 10:19:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:19:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:19:51: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:19:51: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:19:53: 5000000 INFO @ Tue, 14 Jul 2020 10:19:59: 1000000 INFO @ Tue, 14 Jul 2020 10:20:00: 6000000 INFO @ Tue, 14 Jul 2020 10:20:06: 2000000 INFO @ Tue, 14 Jul 2020 10:20:07: 7000000 INFO @ Tue, 14 Jul 2020 10:20:12: 3000000 INFO @ Tue, 14 Jul 2020 10:20:14: 8000000 INFO @ Tue, 14 Jul 2020 10:20:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:20:21: 9000000 INFO @ Tue, 14 Jul 2020 10:20:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:20:22: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:20:22: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:20:26: 5000000 INFO @ Tue, 14 Jul 2020 10:20:28: 1000000 INFO @ Tue, 14 Jul 2020 10:20:28: 10000000 INFO @ Tue, 14 Jul 2020 10:20:32: 6000000 INFO @ Tue, 14 Jul 2020 10:20:35: 2000000 INFO @ Tue, 14 Jul 2020 10:20:35: 11000000 INFO @ Tue, 14 Jul 2020 10:20:39: 7000000 INFO @ Tue, 14 Jul 2020 10:20:42: 3000000 INFO @ Tue, 14 Jul 2020 10:20:43: 12000000 INFO @ Tue, 14 Jul 2020 10:20:45: 8000000 INFO @ Tue, 14 Jul 2020 10:20:49: 4000000 INFO @ Tue, 14 Jul 2020 10:20:50: 13000000 INFO @ Tue, 14 Jul 2020 10:20:52: 9000000 INFO @ Tue, 14 Jul 2020 10:20:57: 5000000 INFO @ Tue, 14 Jul 2020 10:20:57: 14000000 INFO @ Tue, 14 Jul 2020 10:20:59: 10000000 INFO @ Tue, 14 Jul 2020 10:21:04: 6000000 INFO @ Tue, 14 Jul 2020 10:21:04: 15000000 INFO @ Tue, 14 Jul 2020 10:21:05: 11000000 INFO @ Tue, 14 Jul 2020 10:21:11: 7000000 INFO @ Tue, 14 Jul 2020 10:21:11: 16000000 INFO @ Tue, 14 Jul 2020 10:21:12: 12000000 INFO @ Tue, 14 Jul 2020 10:21:18: 13000000 INFO @ Tue, 14 Jul 2020 10:21:18: 8000000 INFO @ Tue, 14 Jul 2020 10:21:19: 17000000 INFO @ Tue, 14 Jul 2020 10:21:25: 14000000 INFO @ Tue, 14 Jul 2020 10:21:26: 9000000 INFO @ Tue, 14 Jul 2020 10:21:26: 18000000 INFO @ Tue, 14 Jul 2020 10:21:31: 15000000 INFO @ Tue, 14 Jul 2020 10:21:33: 10000000 INFO @ Tue, 14 Jul 2020 10:21:33: 19000000 INFO @ Tue, 14 Jul 2020 10:21:38: 16000000 INFO @ Tue, 14 Jul 2020 10:21:40: 11000000 INFO @ Tue, 14 Jul 2020 10:21:40: 20000000 INFO @ Tue, 14 Jul 2020 10:21:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:21:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:21:43: #1 total tags in treatment: 20333848 INFO @ Tue, 14 Jul 2020 10:21:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:21:43: #1 tags after filtering in treatment: 20333790 INFO @ Tue, 14 Jul 2020 10:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:21:43: #1 finished! INFO @ Tue, 14 Jul 2020 10:21:43: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:21:45: 17000000 INFO @ Tue, 14 Jul 2020 10:21:45: #2 number of paired peaks: 48 WARNING @ Tue, 14 Jul 2020 10:21:45: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:21:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:21:47: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:21:51: 18000000 INFO @ Tue, 14 Jul 2020 10:21:54: 13000000 INFO @ Tue, 14 Jul 2020 10:21:58: 19000000 INFO @ Tue, 14 Jul 2020 10:22:01: 14000000 INFO @ Tue, 14 Jul 2020 10:22:04: 20000000 INFO @ Tue, 14 Jul 2020 10:22:06: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:22:06: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:22:06: #1 total tags in treatment: 20333848 INFO @ Tue, 14 Jul 2020 10:22:06: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:22:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:22:07: #1 tags after filtering in treatment: 20333790 INFO @ Tue, 14 Jul 2020 10:22:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:22:07: #1 finished! INFO @ Tue, 14 Jul 2020 10:22:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:22:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:22:08: 15000000 INFO @ Tue, 14 Jul 2020 10:22:08: #2 number of paired peaks: 48 WARNING @ Tue, 14 Jul 2020 10:22:08: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:22:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:22:14: 16000000 INFO @ Tue, 14 Jul 2020 10:22:21: 17000000 INFO @ Tue, 14 Jul 2020 10:22:28: 18000000 INFO @ Tue, 14 Jul 2020 10:22:34: 19000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:22:41: 20000000 INFO @ Tue, 14 Jul 2020 10:22:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:22:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:22:43: #1 total tags in treatment: 20333848 INFO @ Tue, 14 Jul 2020 10:22:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:22:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:22:44: #1 tags after filtering in treatment: 20333790 INFO @ Tue, 14 Jul 2020 10:22:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:22:44: #1 finished! INFO @ Tue, 14 Jul 2020 10:22:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:22:45: #2 number of paired peaks: 48 WARNING @ Tue, 14 Jul 2020 10:22:45: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:22:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780066/SRX7780066.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling