Job ID = 6627124 SRX = SRX7780064 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:41 24973530 reads; of these: 24973530 (100.00%) were unpaired; of these: 2839508 (11.37%) aligned 0 times 16389689 (65.63%) aligned exactly 1 time 5744333 (23.00%) aligned >1 times 88.63% overall alignment rate Time searching: 00:06:41 Overall time: 00:06:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5023754 / 22134022 = 0.2270 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:13:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:13:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:13:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:14:04: 1000000 INFO @ Tue, 14 Jul 2020 10:14:11: 2000000 INFO @ Tue, 14 Jul 2020 10:14:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:14:24: 4000000 INFO @ Tue, 14 Jul 2020 10:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:14:25: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:14:25: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:14:29: 5000000 INFO @ Tue, 14 Jul 2020 10:14:31: 1000000 INFO @ Tue, 14 Jul 2020 10:14:35: 6000000 INFO @ Tue, 14 Jul 2020 10:14:37: 2000000 INFO @ Tue, 14 Jul 2020 10:14:40: 7000000 INFO @ Tue, 14 Jul 2020 10:14:42: 3000000 INFO @ Tue, 14 Jul 2020 10:14:46: 8000000 INFO @ Tue, 14 Jul 2020 10:14:48: 4000000 INFO @ Tue, 14 Jul 2020 10:14:51: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:14:54: 5000000 INFO @ Tue, 14 Jul 2020 10:14:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:14:55: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:14:55: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:14:57: 10000000 INFO @ Tue, 14 Jul 2020 10:14:59: 6000000 INFO @ Tue, 14 Jul 2020 10:15:00: 1000000 INFO @ Tue, 14 Jul 2020 10:15:02: 11000000 INFO @ Tue, 14 Jul 2020 10:15:05: 2000000 INFO @ Tue, 14 Jul 2020 10:15:05: 7000000 INFO @ Tue, 14 Jul 2020 10:15:08: 12000000 INFO @ Tue, 14 Jul 2020 10:15:10: 3000000 INFO @ Tue, 14 Jul 2020 10:15:11: 8000000 INFO @ Tue, 14 Jul 2020 10:15:13: 13000000 INFO @ Tue, 14 Jul 2020 10:15:15: 4000000 INFO @ Tue, 14 Jul 2020 10:15:16: 9000000 INFO @ Tue, 14 Jul 2020 10:15:19: 14000000 INFO @ Tue, 14 Jul 2020 10:15:20: 5000000 INFO @ Tue, 14 Jul 2020 10:15:22: 10000000 INFO @ Tue, 14 Jul 2020 10:15:24: 15000000 INFO @ Tue, 14 Jul 2020 10:15:24: 6000000 INFO @ Tue, 14 Jul 2020 10:15:28: 11000000 INFO @ Tue, 14 Jul 2020 10:15:29: 7000000 INFO @ Tue, 14 Jul 2020 10:15:30: 16000000 INFO @ Tue, 14 Jul 2020 10:15:34: 8000000 INFO @ Tue, 14 Jul 2020 10:15:34: 12000000 INFO @ Tue, 14 Jul 2020 10:15:35: 17000000 INFO @ Tue, 14 Jul 2020 10:15:36: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:15:36: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:15:36: #1 total tags in treatment: 17110268 INFO @ Tue, 14 Jul 2020 10:15:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:15:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:15:36: #1 tags after filtering in treatment: 17110192 INFO @ Tue, 14 Jul 2020 10:15:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:15:36: #1 finished! INFO @ Tue, 14 Jul 2020 10:15:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:15:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:15:37: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 10:15:37: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:15:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:15:39: 9000000 INFO @ Tue, 14 Jul 2020 10:15:41: 13000000 INFO @ Tue, 14 Jul 2020 10:15:44: 10000000 INFO @ Tue, 14 Jul 2020 10:15:47: 14000000 INFO @ Tue, 14 Jul 2020 10:15:49: 11000000 INFO @ Tue, 14 Jul 2020 10:15:52: 15000000 INFO @ Tue, 14 Jul 2020 10:15:54: 12000000 INFO @ Tue, 14 Jul 2020 10:15:58: 16000000 INFO @ Tue, 14 Jul 2020 10:15:58: 13000000 INFO @ Tue, 14 Jul 2020 10:16:03: 17000000 INFO @ Tue, 14 Jul 2020 10:16:03: 14000000 INFO @ Tue, 14 Jul 2020 10:16:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:16:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:16:04: #1 total tags in treatment: 17110268 INFO @ Tue, 14 Jul 2020 10:16:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:16:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:16:04: #1 tags after filtering in treatment: 17110192 INFO @ Tue, 14 Jul 2020 10:16:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:16:04: #1 finished! INFO @ Tue, 14 Jul 2020 10:16:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:16:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:16:05: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 10:16:05: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:16:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:16:08: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:16:13: 16000000 INFO @ Tue, 14 Jul 2020 10:16:18: 17000000 INFO @ Tue, 14 Jul 2020 10:16:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:16:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:16:18: #1 total tags in treatment: 17110268 INFO @ Tue, 14 Jul 2020 10:16:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:16:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:16:19: #1 tags after filtering in treatment: 17110192 INFO @ Tue, 14 Jul 2020 10:16:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:16:19: #1 finished! INFO @ Tue, 14 Jul 2020 10:16:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:16:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:16:20: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 10:16:20: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:16:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780064/SRX7780064.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。