Job ID = 6627134 SRX = SRX7780061 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:08 24110038 reads; of these: 24110038 (100.00%) were unpaired; of these: 1980658 (8.22%) aligned 0 times 16634474 (68.99%) aligned exactly 1 time 5494906 (22.79%) aligned >1 times 91.78% overall alignment rate Time searching: 00:07:08 Overall time: 00:07:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4989215 / 22129380 = 0.2255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:24:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:24:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:24:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:24:41: 1000000 INFO @ Tue, 14 Jul 2020 10:24:47: 2000000 INFO @ Tue, 14 Jul 2020 10:24:53: 3000000 INFO @ Tue, 14 Jul 2020 10:24:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:25:04: 5000000 INFO @ Tue, 14 Jul 2020 10:25:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:25:05: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:25:05: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:25:10: 6000000 INFO @ Tue, 14 Jul 2020 10:25:11: 1000000 INFO @ Tue, 14 Jul 2020 10:25:16: 7000000 INFO @ Tue, 14 Jul 2020 10:25:17: 2000000 INFO @ Tue, 14 Jul 2020 10:25:22: 8000000 INFO @ Tue, 14 Jul 2020 10:25:24: 3000000 INFO @ Tue, 14 Jul 2020 10:25:29: 9000000 INFO @ Tue, 14 Jul 2020 10:25:31: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:25:35: 10000000 INFO @ Tue, 14 Jul 2020 10:25:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:25:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:25:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:25:39: 5000000 INFO @ Tue, 14 Jul 2020 10:25:41: 11000000 INFO @ Tue, 14 Jul 2020 10:25:43: 1000000 INFO @ Tue, 14 Jul 2020 10:25:46: 6000000 INFO @ Tue, 14 Jul 2020 10:25:47: 12000000 INFO @ Tue, 14 Jul 2020 10:25:50: 2000000 INFO @ Tue, 14 Jul 2020 10:25:54: 13000000 INFO @ Tue, 14 Jul 2020 10:25:55: 7000000 INFO @ Tue, 14 Jul 2020 10:25:56: 3000000 INFO @ Tue, 14 Jul 2020 10:26:00: 14000000 INFO @ Tue, 14 Jul 2020 10:26:03: 8000000 INFO @ Tue, 14 Jul 2020 10:26:03: 4000000 INFO @ Tue, 14 Jul 2020 10:26:07: 15000000 INFO @ Tue, 14 Jul 2020 10:26:10: 5000000 INFO @ Tue, 14 Jul 2020 10:26:11: 9000000 INFO @ Tue, 14 Jul 2020 10:26:13: 16000000 INFO @ Tue, 14 Jul 2020 10:26:17: 6000000 INFO @ Tue, 14 Jul 2020 10:26:19: 17000000 INFO @ Tue, 14 Jul 2020 10:26:19: 10000000 INFO @ Tue, 14 Jul 2020 10:26:21: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:26:21: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:26:21: #1 total tags in treatment: 17140165 INFO @ Tue, 14 Jul 2020 10:26:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:26:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:26:21: #1 tags after filtering in treatment: 17140099 INFO @ Tue, 14 Jul 2020 10:26:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:26:21: #1 finished! INFO @ Tue, 14 Jul 2020 10:26:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:26:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:26:23: #2 number of paired peaks: 34 WARNING @ Tue, 14 Jul 2020 10:26:23: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:26:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:26:24: 7000000 INFO @ Tue, 14 Jul 2020 10:26:28: 11000000 INFO @ Tue, 14 Jul 2020 10:26:32: 8000000 INFO @ Tue, 14 Jul 2020 10:26:36: 12000000 INFO @ Tue, 14 Jul 2020 10:26:39: 9000000 INFO @ Tue, 14 Jul 2020 10:26:44: 13000000 INFO @ Tue, 14 Jul 2020 10:26:46: 10000000 INFO @ Tue, 14 Jul 2020 10:26:52: 14000000 INFO @ Tue, 14 Jul 2020 10:26:53: 11000000 INFO @ Tue, 14 Jul 2020 10:27:00: 12000000 INFO @ Tue, 14 Jul 2020 10:27:00: 15000000 INFO @ Tue, 14 Jul 2020 10:27:07: 13000000 INFO @ Tue, 14 Jul 2020 10:27:08: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:27:14: 14000000 INFO @ Tue, 14 Jul 2020 10:27:16: 17000000 INFO @ Tue, 14 Jul 2020 10:27:17: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:27:17: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:27:17: #1 total tags in treatment: 17140165 INFO @ Tue, 14 Jul 2020 10:27:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:27:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:27:18: #1 tags after filtering in treatment: 17140099 INFO @ Tue, 14 Jul 2020 10:27:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:27:18: #1 finished! INFO @ Tue, 14 Jul 2020 10:27:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:27:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:27:19: #2 number of paired peaks: 34 WARNING @ Tue, 14 Jul 2020 10:27:19: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:27:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:27:21: 15000000 INFO @ Tue, 14 Jul 2020 10:27:28: 16000000 INFO @ Tue, 14 Jul 2020 10:27:35: 17000000 INFO @ Tue, 14 Jul 2020 10:27:36: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:27:36: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:27:36: #1 total tags in treatment: 17140165 INFO @ Tue, 14 Jul 2020 10:27:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:27:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:27:37: #1 tags after filtering in treatment: 17140099 INFO @ Tue, 14 Jul 2020 10:27:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:27:37: #1 finished! INFO @ Tue, 14 Jul 2020 10:27:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:27:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:27:38: #2 number of paired peaks: 34 WARNING @ Tue, 14 Jul 2020 10:27:38: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:27:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780061/SRX7780061.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。