Job ID = 6627140 SRX = SRX7780059 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:04 42379724 reads; of these: 42379724 (100.00%) were unpaired; of these: 3838551 (9.06%) aligned 0 times 29023231 (68.48%) aligned exactly 1 time 9517942 (22.46%) aligned >1 times 90.94% overall alignment rate Time searching: 00:11:04 Overall time: 00:11:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12464741 / 38541173 = 0.3234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:32:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:32:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:32:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:32:21: 1000000 INFO @ Tue, 14 Jul 2020 10:32:27: 2000000 INFO @ Tue, 14 Jul 2020 10:32:33: 3000000 INFO @ Tue, 14 Jul 2020 10:32:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:32:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:32:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:32:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:32:45: 5000000 INFO @ Tue, 14 Jul 2020 10:32:51: 1000000 INFO @ Tue, 14 Jul 2020 10:32:52: 6000000 INFO @ Tue, 14 Jul 2020 10:32:58: 2000000 INFO @ Tue, 14 Jul 2020 10:32:58: 7000000 INFO @ Tue, 14 Jul 2020 10:33:04: 3000000 INFO @ Tue, 14 Jul 2020 10:33:05: 8000000 INFO @ Tue, 14 Jul 2020 10:33:10: 4000000 INFO @ Tue, 14 Jul 2020 10:33:11: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:33:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:33:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:33:17: 5000000 INFO @ Tue, 14 Jul 2020 10:33:18: 10000000 INFO @ Tue, 14 Jul 2020 10:33:21: 1000000 INFO @ Tue, 14 Jul 2020 10:33:23: 6000000 INFO @ Tue, 14 Jul 2020 10:33:24: 11000000 INFO @ Tue, 14 Jul 2020 10:33:27: 2000000 INFO @ Tue, 14 Jul 2020 10:33:29: 7000000 INFO @ Tue, 14 Jul 2020 10:33:31: 12000000 INFO @ Tue, 14 Jul 2020 10:33:33: 3000000 INFO @ Tue, 14 Jul 2020 10:33:36: 8000000 INFO @ Tue, 14 Jul 2020 10:33:37: 13000000 INFO @ Tue, 14 Jul 2020 10:33:39: 4000000 INFO @ Tue, 14 Jul 2020 10:33:42: 9000000 INFO @ Tue, 14 Jul 2020 10:33:44: 14000000 INFO @ Tue, 14 Jul 2020 10:33:45: 5000000 INFO @ Tue, 14 Jul 2020 10:33:49: 10000000 INFO @ Tue, 14 Jul 2020 10:33:51: 15000000 INFO @ Tue, 14 Jul 2020 10:33:51: 6000000 INFO @ Tue, 14 Jul 2020 10:33:55: 11000000 INFO @ Tue, 14 Jul 2020 10:33:57: 7000000 INFO @ Tue, 14 Jul 2020 10:33:57: 16000000 INFO @ Tue, 14 Jul 2020 10:34:02: 12000000 INFO @ Tue, 14 Jul 2020 10:34:03: 8000000 INFO @ Tue, 14 Jul 2020 10:34:04: 17000000 INFO @ Tue, 14 Jul 2020 10:34:08: 13000000 INFO @ Tue, 14 Jul 2020 10:34:09: 9000000 INFO @ Tue, 14 Jul 2020 10:34:10: 18000000 INFO @ Tue, 14 Jul 2020 10:34:15: 14000000 INFO @ Tue, 14 Jul 2020 10:34:15: 10000000 INFO @ Tue, 14 Jul 2020 10:34:17: 19000000 INFO @ Tue, 14 Jul 2020 10:34:21: 11000000 INFO @ Tue, 14 Jul 2020 10:34:21: 15000000 INFO @ Tue, 14 Jul 2020 10:34:24: 20000000 INFO @ Tue, 14 Jul 2020 10:34:26: 12000000 INFO @ Tue, 14 Jul 2020 10:34:27: 16000000 INFO @ Tue, 14 Jul 2020 10:34:30: 21000000 INFO @ Tue, 14 Jul 2020 10:34:32: 13000000 INFO @ Tue, 14 Jul 2020 10:34:34: 17000000 INFO @ Tue, 14 Jul 2020 10:34:37: 22000000 INFO @ Tue, 14 Jul 2020 10:34:38: 14000000 INFO @ Tue, 14 Jul 2020 10:34:40: 18000000 INFO @ Tue, 14 Jul 2020 10:34:44: 23000000 INFO @ Tue, 14 Jul 2020 10:34:44: 15000000 INFO @ Tue, 14 Jul 2020 10:34:47: 19000000 INFO @ Tue, 14 Jul 2020 10:34:50: 16000000 INFO @ Tue, 14 Jul 2020 10:34:50: 24000000 INFO @ Tue, 14 Jul 2020 10:34:53: 20000000 INFO @ Tue, 14 Jul 2020 10:34:55: 17000000 INFO @ Tue, 14 Jul 2020 10:34:57: 25000000 INFO @ Tue, 14 Jul 2020 10:35:00: 21000000 INFO @ Tue, 14 Jul 2020 10:35:01: 18000000 INFO @ Tue, 14 Jul 2020 10:35:03: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:35:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:35:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:35:04: #1 total tags in treatment: 26076432 INFO @ Tue, 14 Jul 2020 10:35:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:35:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:35:05: #1 tags after filtering in treatment: 26076391 INFO @ Tue, 14 Jul 2020 10:35:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:35:05: #1 finished! INFO @ Tue, 14 Jul 2020 10:35:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:35:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:35:06: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 10:35:06: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:35:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:35:06: 22000000 INFO @ Tue, 14 Jul 2020 10:35:07: 19000000 INFO @ Tue, 14 Jul 2020 10:35:13: 23000000 INFO @ Tue, 14 Jul 2020 10:35:13: 20000000 INFO @ Tue, 14 Jul 2020 10:35:19: 21000000 INFO @ Tue, 14 Jul 2020 10:35:19: 24000000 INFO @ Tue, 14 Jul 2020 10:35:25: 22000000 INFO @ Tue, 14 Jul 2020 10:35:25: 25000000 INFO @ Tue, 14 Jul 2020 10:35:30: 23000000 INFO @ Tue, 14 Jul 2020 10:35:32: 26000000 INFO @ Tue, 14 Jul 2020 10:35:32: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:35:32: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:35:32: #1 total tags in treatment: 26076432 INFO @ Tue, 14 Jul 2020 10:35:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:35:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:35:33: #1 tags after filtering in treatment: 26076391 INFO @ Tue, 14 Jul 2020 10:35:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:35:33: #1 finished! INFO @ Tue, 14 Jul 2020 10:35:33: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:35:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:35:34: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 10:35:34: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:35:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:35:36: 24000000 INFO @ Tue, 14 Jul 2020 10:35:41: 25000000 INFO @ Tue, 14 Jul 2020 10:35:47: 26000000 INFO @ Tue, 14 Jul 2020 10:35:47: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:35:47: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:35:47: #1 total tags in treatment: 26076432 INFO @ Tue, 14 Jul 2020 10:35:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:35:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:35:48: #1 tags after filtering in treatment: 26076391 INFO @ Tue, 14 Jul 2020 10:35:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:35:48: #1 finished! INFO @ Tue, 14 Jul 2020 10:35:48: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:35:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:35:50: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 10:35:50: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:35:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780059/SRX7780059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。