Job ID = 6627106 SRX = SRX7780057 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:28 28771525 reads; of these: 28771525 (100.00%) were unpaired; of these: 1989224 (6.91%) aligned 0 times 19751004 (68.65%) aligned exactly 1 time 7031297 (24.44%) aligned >1 times 93.09% overall alignment rate Time searching: 00:07:28 Overall time: 00:07:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6762074 / 26782301 = 0.2525 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:08:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:08:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:08:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:08:37: 1000000 INFO @ Tue, 14 Jul 2020 10:08:43: 2000000 INFO @ Tue, 14 Jul 2020 10:08:50: 3000000 INFO @ Tue, 14 Jul 2020 10:08:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:09:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:09:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:09:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:09:02: 5000000 INFO @ Tue, 14 Jul 2020 10:09:07: 1000000 INFO @ Tue, 14 Jul 2020 10:09:09: 6000000 INFO @ Tue, 14 Jul 2020 10:09:14: 2000000 INFO @ Tue, 14 Jul 2020 10:09:15: 7000000 INFO @ Tue, 14 Jul 2020 10:09:20: 3000000 INFO @ Tue, 14 Jul 2020 10:09:21: 8000000 INFO @ Tue, 14 Jul 2020 10:09:27: 4000000 INFO @ Tue, 14 Jul 2020 10:09:28: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:09:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:09:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:09:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:09:33: 5000000 INFO @ Tue, 14 Jul 2020 10:09:34: 10000000 INFO @ Tue, 14 Jul 2020 10:09:37: 1000000 INFO @ Tue, 14 Jul 2020 10:09:40: 6000000 INFO @ Tue, 14 Jul 2020 10:09:41: 11000000 INFO @ Tue, 14 Jul 2020 10:09:44: 2000000 INFO @ Tue, 14 Jul 2020 10:09:46: 7000000 INFO @ Tue, 14 Jul 2020 10:09:47: 12000000 INFO @ Tue, 14 Jul 2020 10:09:50: 3000000 INFO @ Tue, 14 Jul 2020 10:09:52: 8000000 INFO @ Tue, 14 Jul 2020 10:09:54: 13000000 INFO @ Tue, 14 Jul 2020 10:09:57: 4000000 INFO @ Tue, 14 Jul 2020 10:09:59: 9000000 INFO @ Tue, 14 Jul 2020 10:10:01: 14000000 INFO @ Tue, 14 Jul 2020 10:10:03: 5000000 INFO @ Tue, 14 Jul 2020 10:10:05: 10000000 INFO @ Tue, 14 Jul 2020 10:10:07: 15000000 INFO @ Tue, 14 Jul 2020 10:10:09: 6000000 INFO @ Tue, 14 Jul 2020 10:10:12: 11000000 INFO @ Tue, 14 Jul 2020 10:10:14: 16000000 INFO @ Tue, 14 Jul 2020 10:10:15: 7000000 INFO @ Tue, 14 Jul 2020 10:10:19: 12000000 INFO @ Tue, 14 Jul 2020 10:10:21: 17000000 INFO @ Tue, 14 Jul 2020 10:10:22: 8000000 INFO @ Tue, 14 Jul 2020 10:10:25: 13000000 INFO @ Tue, 14 Jul 2020 10:10:27: 18000000 INFO @ Tue, 14 Jul 2020 10:10:28: 9000000 INFO @ Tue, 14 Jul 2020 10:10:32: 14000000 INFO @ Tue, 14 Jul 2020 10:10:34: 19000000 INFO @ Tue, 14 Jul 2020 10:10:34: 10000000 INFO @ Tue, 14 Jul 2020 10:10:39: 15000000 INFO @ Tue, 14 Jul 2020 10:10:40: 20000000 INFO @ Tue, 14 Jul 2020 10:10:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:10:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:10:41: #1 total tags in treatment: 20020227 INFO @ Tue, 14 Jul 2020 10:10:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:10:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:10:41: 11000000 INFO @ Tue, 14 Jul 2020 10:10:41: #1 tags after filtering in treatment: 20020189 INFO @ Tue, 14 Jul 2020 10:10:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:10:41: #1 finished! INFO @ Tue, 14 Jul 2020 10:10:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:10:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:10:43: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 10:10:43: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:10:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:10:45: 16000000 INFO @ Tue, 14 Jul 2020 10:10:47: 12000000 INFO @ Tue, 14 Jul 2020 10:10:52: 17000000 INFO @ Tue, 14 Jul 2020 10:10:54: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:10:59: 18000000 INFO @ Tue, 14 Jul 2020 10:11:00: 14000000 INFO @ Tue, 14 Jul 2020 10:11:05: 19000000 INFO @ Tue, 14 Jul 2020 10:11:07: 15000000 INFO @ Tue, 14 Jul 2020 10:11:12: 20000000 INFO @ Tue, 14 Jul 2020 10:11:12: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:11:12: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:11:12: #1 total tags in treatment: 20020227 INFO @ Tue, 14 Jul 2020 10:11:12: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:11:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:11:13: #1 tags after filtering in treatment: 20020189 INFO @ Tue, 14 Jul 2020 10:11:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:11:13: #1 finished! INFO @ Tue, 14 Jul 2020 10:11:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:11:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:11:13: 16000000 INFO @ Tue, 14 Jul 2020 10:11:14: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 10:11:14: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:11:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:11:20: 17000000 INFO @ Tue, 14 Jul 2020 10:11:26: 18000000 INFO @ Tue, 14 Jul 2020 10:11:32: 19000000 INFO @ Tue, 14 Jul 2020 10:11:38: 20000000 INFO @ Tue, 14 Jul 2020 10:11:38: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:11:38: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:11:38: #1 total tags in treatment: 20020227 INFO @ Tue, 14 Jul 2020 10:11:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:11:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:11:39: #1 tags after filtering in treatment: 20020189 INFO @ Tue, 14 Jul 2020 10:11:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:11:39: #1 finished! INFO @ Tue, 14 Jul 2020 10:11:39: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:11:39: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:11:40: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 10:11:40: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:11:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780057/SRX7780057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling