Job ID = 6627187 SRX = SRX7780053 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:20 30093259 reads; of these: 30093259 (100.00%) were unpaired; of these: 2536162 (8.43%) aligned 0 times 20500260 (68.12%) aligned exactly 1 time 7056837 (23.45%) aligned >1 times 91.57% overall alignment rate Time searching: 00:07:20 Overall time: 00:07:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7235380 / 27557097 = 0.2626 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:43:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:43:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:43:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:43:18: 1000000 INFO @ Tue, 14 Jul 2020 10:43:23: 2000000 INFO @ Tue, 14 Jul 2020 10:43:28: 3000000 INFO @ Tue, 14 Jul 2020 10:43:33: 4000000 INFO @ Tue, 14 Jul 2020 10:43:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:43:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:43:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:43:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:43:43: 6000000 INFO @ Tue, 14 Jul 2020 10:43:49: 1000000 INFO @ Tue, 14 Jul 2020 10:43:49: 7000000 INFO @ Tue, 14 Jul 2020 10:43:54: 2000000 INFO @ Tue, 14 Jul 2020 10:43:54: 8000000 INFO @ Tue, 14 Jul 2020 10:44:00: 3000000 INFO @ Tue, 14 Jul 2020 10:44:00: 9000000 INFO @ Tue, 14 Jul 2020 10:44:05: 10000000 INFO @ Tue, 14 Jul 2020 10:44:05: 4000000 INFO @ Tue, 14 Jul 2020 10:44:11: 11000000 INFO @ Tue, 14 Jul 2020 10:44:11: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:44:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:44:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:44:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:44:16: 12000000 INFO @ Tue, 14 Jul 2020 10:44:17: 6000000 INFO @ Tue, 14 Jul 2020 10:44:20: 1000000 INFO @ Tue, 14 Jul 2020 10:44:22: 13000000 INFO @ Tue, 14 Jul 2020 10:44:23: 7000000 INFO @ Tue, 14 Jul 2020 10:44:27: 2000000 INFO @ Tue, 14 Jul 2020 10:44:28: 14000000 INFO @ Tue, 14 Jul 2020 10:44:29: 8000000 INFO @ Tue, 14 Jul 2020 10:44:33: 15000000 INFO @ Tue, 14 Jul 2020 10:44:35: 3000000 INFO @ Tue, 14 Jul 2020 10:44:35: 9000000 INFO @ Tue, 14 Jul 2020 10:44:39: 16000000 INFO @ Tue, 14 Jul 2020 10:44:40: 10000000 INFO @ Tue, 14 Jul 2020 10:44:44: 4000000 INFO @ Tue, 14 Jul 2020 10:44:45: 17000000 INFO @ Tue, 14 Jul 2020 10:44:47: 11000000 INFO @ Tue, 14 Jul 2020 10:44:50: 18000000 INFO @ Tue, 14 Jul 2020 10:44:50: 5000000 INFO @ Tue, 14 Jul 2020 10:44:54: 12000000 INFO @ Tue, 14 Jul 2020 10:44:56: 19000000 INFO @ Tue, 14 Jul 2020 10:44:57: 6000000 INFO @ Tue, 14 Jul 2020 10:45:01: 13000000 INFO @ Tue, 14 Jul 2020 10:45:02: 20000000 INFO @ Tue, 14 Jul 2020 10:45:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:45:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:45:04: #1 total tags in treatment: 20321717 INFO @ Tue, 14 Jul 2020 10:45:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:45:04: 7000000 INFO @ Tue, 14 Jul 2020 10:45:04: #1 tags after filtering in treatment: 20321668 INFO @ Tue, 14 Jul 2020 10:45:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:45:04: #1 finished! INFO @ Tue, 14 Jul 2020 10:45:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:45:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:45:05: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:45:05: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:45:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:45:08: 14000000 INFO @ Tue, 14 Jul 2020 10:45:10: 8000000 INFO @ Tue, 14 Jul 2020 10:45:15: 15000000 INFO @ Tue, 14 Jul 2020 10:45:17: 9000000 INFO @ Tue, 14 Jul 2020 10:45:22: 16000000 INFO @ Tue, 14 Jul 2020 10:45:23: 10000000 INFO @ Tue, 14 Jul 2020 10:45:30: 17000000 INFO @ Tue, 14 Jul 2020 10:45:30: 11000000 INFO @ Tue, 14 Jul 2020 10:45:37: 18000000 INFO @ Tue, 14 Jul 2020 10:45:37: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:45:44: 13000000 INFO @ Tue, 14 Jul 2020 10:45:44: 19000000 INFO @ Tue, 14 Jul 2020 10:45:50: 14000000 INFO @ Tue, 14 Jul 2020 10:45:51: 20000000 INFO @ Tue, 14 Jul 2020 10:45:53: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:45:53: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:45:53: #1 total tags in treatment: 20321717 INFO @ Tue, 14 Jul 2020 10:45:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:45:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:45:53: #1 tags after filtering in treatment: 20321668 INFO @ Tue, 14 Jul 2020 10:45:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:45:53: #1 finished! INFO @ Tue, 14 Jul 2020 10:45:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:45:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:45:55: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:45:55: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:45:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:45:56: 15000000 INFO @ Tue, 14 Jul 2020 10:46:02: 16000000 INFO @ Tue, 14 Jul 2020 10:46:09: 17000000 INFO @ Tue, 14 Jul 2020 10:46:15: 18000000 INFO @ Tue, 14 Jul 2020 10:46:21: 19000000 INFO @ Tue, 14 Jul 2020 10:46:27: 20000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:46:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:46:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:46:29: #1 total tags in treatment: 20321717 INFO @ Tue, 14 Jul 2020 10:46:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:46:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:46:29: #1 tags after filtering in treatment: 20321668 INFO @ Tue, 14 Jul 2020 10:46:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:46:29: #1 finished! INFO @ Tue, 14 Jul 2020 10:46:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:46:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:46:31: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:46:31: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:46:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780053/SRX7780053.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling