Job ID = 6627097 SRX = SRX7780052 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:28 24153088 reads; of these: 24153088 (100.00%) were unpaired; of these: 2698564 (11.17%) aligned 0 times 15735993 (65.15%) aligned exactly 1 time 5718531 (23.68%) aligned >1 times 88.83% overall alignment rate Time searching: 00:06:28 Overall time: 00:06:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4788406 / 21454524 = 0.2232 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:02:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:02:44: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:02:44: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:02:49: 1000000 INFO @ Tue, 14 Jul 2020 10:02:55: 2000000 INFO @ Tue, 14 Jul 2020 10:03:00: 3000000 INFO @ Tue, 14 Jul 2020 10:03:06: 4000000 INFO @ Tue, 14 Jul 2020 10:03:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:03:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:03:14: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:03:14: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:03:17: 6000000 INFO @ Tue, 14 Jul 2020 10:03:19: 1000000 INFO @ Tue, 14 Jul 2020 10:03:22: 7000000 INFO @ Tue, 14 Jul 2020 10:03:24: 2000000 INFO @ Tue, 14 Jul 2020 10:03:27: 8000000 INFO @ Tue, 14 Jul 2020 10:03:29: 3000000 INFO @ Tue, 14 Jul 2020 10:03:33: 9000000 INFO @ Tue, 14 Jul 2020 10:03:34: 4000000 INFO @ Tue, 14 Jul 2020 10:03:38: 10000000 INFO @ Tue, 14 Jul 2020 10:03:38: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:03:43: 6000000 INFO @ Tue, 14 Jul 2020 10:03:44: 11000000 INFO @ Tue, 14 Jul 2020 10:03:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:03:44: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:03:44: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:03:48: 7000000 INFO @ Tue, 14 Jul 2020 10:03:49: 12000000 INFO @ Tue, 14 Jul 2020 10:03:50: 1000000 INFO @ Tue, 14 Jul 2020 10:03:53: 8000000 INFO @ Tue, 14 Jul 2020 10:03:55: 13000000 INFO @ Tue, 14 Jul 2020 10:03:56: 2000000 INFO @ Tue, 14 Jul 2020 10:03:58: 9000000 INFO @ Tue, 14 Jul 2020 10:04:01: 14000000 INFO @ Tue, 14 Jul 2020 10:04:02: 3000000 INFO @ Tue, 14 Jul 2020 10:04:02: 10000000 INFO @ Tue, 14 Jul 2020 10:04:06: 15000000 INFO @ Tue, 14 Jul 2020 10:04:07: 11000000 INFO @ Tue, 14 Jul 2020 10:04:08: 4000000 INFO @ Tue, 14 Jul 2020 10:04:12: 16000000 INFO @ Tue, 14 Jul 2020 10:04:12: 12000000 INFO @ Tue, 14 Jul 2020 10:04:13: 5000000 INFO @ Tue, 14 Jul 2020 10:04:16: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:04:16: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:04:16: #1 total tags in treatment: 16666118 INFO @ Tue, 14 Jul 2020 10:04:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:04:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:04:16: #1 tags after filtering in treatment: 16666042 INFO @ Tue, 14 Jul 2020 10:04:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:04:16: #1 finished! INFO @ Tue, 14 Jul 2020 10:04:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:04:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:04:17: 13000000 INFO @ Tue, 14 Jul 2020 10:04:17: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 10:04:17: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:04:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:04:19: 6000000 INFO @ Tue, 14 Jul 2020 10:04:22: 14000000 INFO @ Tue, 14 Jul 2020 10:04:25: 7000000 INFO @ Tue, 14 Jul 2020 10:04:27: 15000000 INFO @ Tue, 14 Jul 2020 10:04:30: 8000000 INFO @ Tue, 14 Jul 2020 10:04:31: 16000000 INFO @ Tue, 14 Jul 2020 10:04:35: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:04:35: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:04:35: #1 total tags in treatment: 16666118 INFO @ Tue, 14 Jul 2020 10:04:35: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:04:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:04:35: #1 tags after filtering in treatment: 16666042 INFO @ Tue, 14 Jul 2020 10:04:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:04:35: #1 finished! INFO @ Tue, 14 Jul 2020 10:04:35: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:04:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:04:36: 9000000 INFO @ Tue, 14 Jul 2020 10:04:36: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 10:04:36: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:04:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:04:41: 10000000 INFO @ Tue, 14 Jul 2020 10:04:47: 11000000 INFO @ Tue, 14 Jul 2020 10:04:52: 12000000 INFO @ Tue, 14 Jul 2020 10:04:58: 13000000 INFO @ Tue, 14 Jul 2020 10:05:03: 14000000 INFO @ Tue, 14 Jul 2020 10:05:09: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:05:14: 16000000 INFO @ Tue, 14 Jul 2020 10:05:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:05:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:05:18: #1 total tags in treatment: 16666118 INFO @ Tue, 14 Jul 2020 10:05:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:05:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:05:19: #1 tags after filtering in treatment: 16666042 INFO @ Tue, 14 Jul 2020 10:05:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:05:19: #1 finished! INFO @ Tue, 14 Jul 2020 10:05:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:05:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:05:20: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 10:05:20: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:05:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780052/SRX7780052.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。