Job ID = 6627083 SRX = SRX7780046 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:32 25758396 reads; of these: 25758396 (100.00%) were unpaired; of these: 2872485 (11.15%) aligned 0 times 16881464 (65.54%) aligned exactly 1 time 6004447 (23.31%) aligned >1 times 88.85% overall alignment rate Time searching: 00:06:32 Overall time: 00:06:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5312320 / 22885911 = 0.2321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:58:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:58:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:58:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:59:00: 1000000 INFO @ Tue, 14 Jul 2020 09:59:05: 2000000 INFO @ Tue, 14 Jul 2020 09:59:10: 3000000 INFO @ Tue, 14 Jul 2020 09:59:16: 4000000 INFO @ Tue, 14 Jul 2020 09:59:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:59:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:59:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:59:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:59:26: 6000000 INFO @ Tue, 14 Jul 2020 09:59:30: 1000000 INFO @ Tue, 14 Jul 2020 09:59:32: 7000000 INFO @ Tue, 14 Jul 2020 09:59:35: 2000000 INFO @ Tue, 14 Jul 2020 09:59:37: 8000000 INFO @ Tue, 14 Jul 2020 09:59:40: 3000000 INFO @ Tue, 14 Jul 2020 09:59:42: 9000000 INFO @ Tue, 14 Jul 2020 09:59:46: 4000000 INFO @ Tue, 14 Jul 2020 09:59:48: 10000000 INFO @ Tue, 14 Jul 2020 09:59:51: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:59:53: 11000000 INFO @ Tue, 14 Jul 2020 09:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:59:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:59:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:59:57: 6000000 INFO @ Tue, 14 Jul 2020 09:59:59: 12000000 INFO @ Tue, 14 Jul 2020 10:00:00: 1000000 INFO @ Tue, 14 Jul 2020 10:00:02: 7000000 INFO @ Tue, 14 Jul 2020 10:00:04: 13000000 INFO @ Tue, 14 Jul 2020 10:00:06: 2000000 INFO @ Tue, 14 Jul 2020 10:00:08: 8000000 INFO @ Tue, 14 Jul 2020 10:00:10: 14000000 INFO @ Tue, 14 Jul 2020 10:00:11: 3000000 INFO @ Tue, 14 Jul 2020 10:00:13: 9000000 INFO @ Tue, 14 Jul 2020 10:00:16: 15000000 INFO @ Tue, 14 Jul 2020 10:00:17: 4000000 INFO @ Tue, 14 Jul 2020 10:00:19: 10000000 INFO @ Tue, 14 Jul 2020 10:00:21: 16000000 INFO @ Tue, 14 Jul 2020 10:00:22: 5000000 INFO @ Tue, 14 Jul 2020 10:00:24: 11000000 INFO @ Tue, 14 Jul 2020 10:00:27: 17000000 INFO @ Tue, 14 Jul 2020 10:00:28: 6000000 INFO @ Tue, 14 Jul 2020 10:00:30: 12000000 INFO @ Tue, 14 Jul 2020 10:00:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:00:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:00:30: #1 total tags in treatment: 17573591 INFO @ Tue, 14 Jul 2020 10:00:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:00:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:00:31: #1 tags after filtering in treatment: 17573525 INFO @ Tue, 14 Jul 2020 10:00:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:00:31: #1 finished! INFO @ Tue, 14 Jul 2020 10:00:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:00:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:00:32: #2 number of paired peaks: 78 WARNING @ Tue, 14 Jul 2020 10:00:32: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:00:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:00:33: 7000000 INFO @ Tue, 14 Jul 2020 10:00:35: 13000000 INFO @ Tue, 14 Jul 2020 10:00:39: 8000000 INFO @ Tue, 14 Jul 2020 10:00:41: 14000000 INFO @ Tue, 14 Jul 2020 10:00:44: 9000000 INFO @ Tue, 14 Jul 2020 10:00:47: 15000000 INFO @ Tue, 14 Jul 2020 10:00:50: 10000000 INFO @ Tue, 14 Jul 2020 10:00:52: 16000000 INFO @ Tue, 14 Jul 2020 10:00:55: 11000000 INFO @ Tue, 14 Jul 2020 10:00:57: 17000000 INFO @ Tue, 14 Jul 2020 10:01:00: 12000000 INFO @ Tue, 14 Jul 2020 10:01:01: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:01:01: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:01:01: #1 total tags in treatment: 17573591 INFO @ Tue, 14 Jul 2020 10:01:01: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:01:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:01:02: #1 tags after filtering in treatment: 17573525 INFO @ Tue, 14 Jul 2020 10:01:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:01:02: #1 finished! INFO @ Tue, 14 Jul 2020 10:01:02: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:01:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:01:03: #2 number of paired peaks: 78 WARNING @ Tue, 14 Jul 2020 10:01:03: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:01:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:01:06: 13000000 INFO @ Tue, 14 Jul 2020 10:01:11: 14000000 INFO @ Tue, 14 Jul 2020 10:01:17: 15000000 INFO @ Tue, 14 Jul 2020 10:01:22: 16000000 INFO @ Tue, 14 Jul 2020 10:01:27: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:01:31: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 10:01:31: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 10:01:31: #1 total tags in treatment: 17573591 INFO @ Tue, 14 Jul 2020 10:01:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:01:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:01:32: #1 tags after filtering in treatment: 17573525 INFO @ Tue, 14 Jul 2020 10:01:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:01:32: #1 finished! INFO @ Tue, 14 Jul 2020 10:01:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:01:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:01:33: #2 number of paired peaks: 78 WARNING @ Tue, 14 Jul 2020 10:01:33: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:01:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780046/SRX7780046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。