Job ID = 6627069 SRX = SRX7780043 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:31 26636412 reads; of these: 26636412 (100.00%) were unpaired; of these: 8441990 (31.69%) aligned 0 times 15660003 (58.79%) aligned exactly 1 time 2534419 (9.51%) aligned >1 times 68.31% overall alignment rate Time searching: 00:05:31 Overall time: 00:05:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5582299 / 18194422 = 0.3068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:53:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:53:11: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:53:11: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:53:16: 1000000 INFO @ Tue, 14 Jul 2020 09:53:21: 2000000 INFO @ Tue, 14 Jul 2020 09:53:26: 3000000 INFO @ Tue, 14 Jul 2020 09:53:30: 4000000 INFO @ Tue, 14 Jul 2020 09:53:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:53:40: 6000000 INFO @ Tue, 14 Jul 2020 09:53:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:53:41: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:53:41: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:53:45: 7000000 INFO @ Tue, 14 Jul 2020 09:53:47: 1000000 INFO @ Tue, 14 Jul 2020 09:53:50: 8000000 INFO @ Tue, 14 Jul 2020 09:53:53: 2000000 INFO @ Tue, 14 Jul 2020 09:53:55: 9000000 INFO @ Tue, 14 Jul 2020 09:54:00: 3000000 INFO @ Tue, 14 Jul 2020 09:54:01: 10000000 INFO @ Tue, 14 Jul 2020 09:54:06: 11000000 INFO @ Tue, 14 Jul 2020 09:54:08: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:54:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:54:11: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:54:11: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:54:11: 12000000 INFO @ Tue, 14 Jul 2020 09:54:15: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:54:15: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:54:15: #1 total tags in treatment: 12612123 INFO @ Tue, 14 Jul 2020 09:54:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:54:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:54:15: 5000000 INFO @ Tue, 14 Jul 2020 09:54:15: #1 tags after filtering in treatment: 12612038 INFO @ Tue, 14 Jul 2020 09:54:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:54:15: #1 finished! INFO @ Tue, 14 Jul 2020 09:54:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:54:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:54:16: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 09:54:16: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:54:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:54:17: 1000000 INFO @ Tue, 14 Jul 2020 09:54:22: 6000000 INFO @ Tue, 14 Jul 2020 09:54:23: 2000000 INFO @ Tue, 14 Jul 2020 09:54:29: 3000000 INFO @ Tue, 14 Jul 2020 09:54:29: 7000000 INFO @ Tue, 14 Jul 2020 09:54:35: 4000000 INFO @ Tue, 14 Jul 2020 09:54:35: 8000000 INFO @ Tue, 14 Jul 2020 09:54:41: 5000000 INFO @ Tue, 14 Jul 2020 09:54:41: 9000000 INFO @ Tue, 14 Jul 2020 09:54:47: 6000000 INFO @ Tue, 14 Jul 2020 09:54:47: 10000000 INFO @ Tue, 14 Jul 2020 09:54:53: 7000000 INFO @ Tue, 14 Jul 2020 09:54:54: 11000000 INFO @ Tue, 14 Jul 2020 09:54:59: 8000000 INFO @ Tue, 14 Jul 2020 09:55:00: 12000000 INFO @ Tue, 14 Jul 2020 09:55:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:55:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:55:04: #1 total tags in treatment: 12612123 INFO @ Tue, 14 Jul 2020 09:55:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:55:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:55:04: #1 tags after filtering in treatment: 12612038 INFO @ Tue, 14 Jul 2020 09:55:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:55:04: #1 finished! INFO @ Tue, 14 Jul 2020 09:55:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:55:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:55:04: 9000000 INFO @ Tue, 14 Jul 2020 09:55:05: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 09:55:05: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:55:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:55:10: 10000000 INFO @ Tue, 14 Jul 2020 09:55:16: 11000000 INFO @ Tue, 14 Jul 2020 09:55:22: 12000000 INFO @ Tue, 14 Jul 2020 09:55:26: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:55:26: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:55:26: #1 total tags in treatment: 12612123 INFO @ Tue, 14 Jul 2020 09:55:26: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:55:26: #1 tags after filtering in treatment: 12612038 INFO @ Tue, 14 Jul 2020 09:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:55:26: #1 finished! INFO @ Tue, 14 Jul 2020 09:55:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:55:27: #2 number of paired peaks: 96 WARNING @ Tue, 14 Jul 2020 09:55:27: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:55:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780043/SRX7780043.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。