Job ID = 6627070 SRX = SRX7780042 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:58 25070454 reads; of these: 25070454 (100.00%) were unpaired; of these: 2098764 (8.37%) aligned 0 times 17710490 (70.64%) aligned exactly 1 time 5261200 (20.99%) aligned >1 times 91.63% overall alignment rate Time searching: 00:05:58 Overall time: 00:05:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5275153 / 22971690 = 0.2296 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:55:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:55:52: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:55:52: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:55:58: 1000000 INFO @ Tue, 14 Jul 2020 09:56:04: 2000000 INFO @ Tue, 14 Jul 2020 09:56:09: 3000000 INFO @ Tue, 14 Jul 2020 09:56:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:56:21: 5000000 INFO @ Tue, 14 Jul 2020 09:56:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:56:22: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:56:22: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:56:27: 6000000 INFO @ Tue, 14 Jul 2020 09:56:28: 1000000 INFO @ Tue, 14 Jul 2020 09:56:33: 7000000 INFO @ Tue, 14 Jul 2020 09:56:34: 2000000 INFO @ Tue, 14 Jul 2020 09:56:39: 8000000 INFO @ Tue, 14 Jul 2020 09:56:39: 3000000 INFO @ Tue, 14 Jul 2020 09:56:45: 4000000 INFO @ Tue, 14 Jul 2020 09:56:45: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:56:51: 5000000 INFO @ Tue, 14 Jul 2020 09:56:52: 10000000 INFO @ Tue, 14 Jul 2020 09:56:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:56:52: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:56:52: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:56:57: 6000000 INFO @ Tue, 14 Jul 2020 09:56:58: 11000000 INFO @ Tue, 14 Jul 2020 09:56:58: 1000000 INFO @ Tue, 14 Jul 2020 09:57:03: 7000000 INFO @ Tue, 14 Jul 2020 09:57:04: 12000000 INFO @ Tue, 14 Jul 2020 09:57:04: 2000000 INFO @ Tue, 14 Jul 2020 09:57:09: 8000000 INFO @ Tue, 14 Jul 2020 09:57:10: 13000000 INFO @ Tue, 14 Jul 2020 09:57:11: 3000000 INFO @ Tue, 14 Jul 2020 09:57:16: 14000000 INFO @ Tue, 14 Jul 2020 09:57:17: 9000000 INFO @ Tue, 14 Jul 2020 09:57:17: 4000000 INFO @ Tue, 14 Jul 2020 09:57:23: 15000000 INFO @ Tue, 14 Jul 2020 09:57:23: 5000000 INFO @ Tue, 14 Jul 2020 09:57:25: 10000000 INFO @ Tue, 14 Jul 2020 09:57:29: 6000000 INFO @ Tue, 14 Jul 2020 09:57:30: 16000000 INFO @ Tue, 14 Jul 2020 09:57:32: 11000000 INFO @ Tue, 14 Jul 2020 09:57:35: 7000000 INFO @ Tue, 14 Jul 2020 09:57:36: 17000000 INFO @ Tue, 14 Jul 2020 09:57:40: 12000000 INFO @ Tue, 14 Jul 2020 09:57:41: 8000000 INFO @ Tue, 14 Jul 2020 09:57:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:57:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:57:41: #1 total tags in treatment: 17696537 INFO @ Tue, 14 Jul 2020 09:57:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:57:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:57:41: #1 tags after filtering in treatment: 17696477 INFO @ Tue, 14 Jul 2020 09:57:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:57:41: #1 finished! INFO @ Tue, 14 Jul 2020 09:57:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:57:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:57:42: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 09:57:42: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:57:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:57:46: 9000000 INFO @ Tue, 14 Jul 2020 09:57:48: 13000000 INFO @ Tue, 14 Jul 2020 09:57:52: 10000000 INFO @ Tue, 14 Jul 2020 09:57:56: 14000000 INFO @ Tue, 14 Jul 2020 09:57:58: 11000000 INFO @ Tue, 14 Jul 2020 09:58:04: 15000000 INFO @ Tue, 14 Jul 2020 09:58:04: 12000000 INFO @ Tue, 14 Jul 2020 09:58:10: 13000000 INFO @ Tue, 14 Jul 2020 09:58:10: 16000000 INFO @ Tue, 14 Jul 2020 09:58:16: 14000000 INFO @ Tue, 14 Jul 2020 09:58:16: 17000000 INFO @ Tue, 14 Jul 2020 09:58:21: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:58:21: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:58:21: #1 total tags in treatment: 17696537 INFO @ Tue, 14 Jul 2020 09:58:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:58:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:58:21: #1 tags after filtering in treatment: 17696477 INFO @ Tue, 14 Jul 2020 09:58:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:58:21: #1 finished! INFO @ Tue, 14 Jul 2020 09:58:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:58:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:58:22: 15000000 INFO @ Tue, 14 Jul 2020 09:58:22: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 09:58:22: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:58:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:58:28: 16000000 INFO @ Tue, 14 Jul 2020 09:58:34: 17000000 INFO @ Tue, 14 Jul 2020 09:58:38: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:58:38: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:58:38: #1 total tags in treatment: 17696537 INFO @ Tue, 14 Jul 2020 09:58:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:58:39: #1 tags after filtering in treatment: 17696477 INFO @ Tue, 14 Jul 2020 09:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:58:39: #1 finished! INFO @ Tue, 14 Jul 2020 09:58:39: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:58:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:58:40: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 09:58:40: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:58:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780042/SRX7780042.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。