Job ID = 6627049 SRX = SRX7780039 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 20605928 reads; of these: 20605928 (100.00%) were unpaired; of these: 1538937 (7.47%) aligned 0 times 15703476 (76.21%) aligned exactly 1 time 3363515 (16.32%) aligned >1 times 92.53% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4290845 / 19066991 = 0.2250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:46:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:46:32: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:46:32: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:46:39: 1000000 INFO @ Tue, 14 Jul 2020 09:46:46: 2000000 INFO @ Tue, 14 Jul 2020 09:46:53: 3000000 INFO @ Tue, 14 Jul 2020 09:46:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:47:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:47:02: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:47:02: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:47:07: 5000000 INFO @ Tue, 14 Jul 2020 09:47:10: 1000000 INFO @ Tue, 14 Jul 2020 09:47:14: 6000000 INFO @ Tue, 14 Jul 2020 09:47:17: 2000000 INFO @ Tue, 14 Jul 2020 09:47:22: 7000000 INFO @ Tue, 14 Jul 2020 09:47:25: 3000000 INFO @ Tue, 14 Jul 2020 09:47:30: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:47:32: 4000000 INFO @ Tue, 14 Jul 2020 09:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:47:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:47:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:47:37: 9000000 INFO @ Tue, 14 Jul 2020 09:47:40: 5000000 INFO @ Tue, 14 Jul 2020 09:47:40: 1000000 INFO @ Tue, 14 Jul 2020 09:47:45: 10000000 INFO @ Tue, 14 Jul 2020 09:47:48: 2000000 INFO @ Tue, 14 Jul 2020 09:47:48: 6000000 INFO @ Tue, 14 Jul 2020 09:47:52: 11000000 INFO @ Tue, 14 Jul 2020 09:47:56: 3000000 INFO @ Tue, 14 Jul 2020 09:47:58: 7000000 INFO @ Tue, 14 Jul 2020 09:48:00: 12000000 INFO @ Tue, 14 Jul 2020 09:48:06: 4000000 INFO @ Tue, 14 Jul 2020 09:48:07: 8000000 INFO @ Tue, 14 Jul 2020 09:48:08: 13000000 INFO @ Tue, 14 Jul 2020 09:48:14: 9000000 INFO @ Tue, 14 Jul 2020 09:48:16: 14000000 INFO @ Tue, 14 Jul 2020 09:48:16: 5000000 INFO @ Tue, 14 Jul 2020 09:48:21: 10000000 INFO @ Tue, 14 Jul 2020 09:48:22: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:48:22: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:48:22: #1 total tags in treatment: 14776146 INFO @ Tue, 14 Jul 2020 09:48:22: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:48:22: #1 tags after filtering in treatment: 14776057 INFO @ Tue, 14 Jul 2020 09:48:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:48:22: #1 finished! INFO @ Tue, 14 Jul 2020 09:48:22: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:48:23: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 09:48:23: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:48:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:48:26: 6000000 INFO @ Tue, 14 Jul 2020 09:48:29: 11000000 INFO @ Tue, 14 Jul 2020 09:48:35: 7000000 INFO @ Tue, 14 Jul 2020 09:48:36: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:48:44: 13000000 INFO @ Tue, 14 Jul 2020 09:48:45: 8000000 INFO @ Tue, 14 Jul 2020 09:48:51: 14000000 INFO @ Tue, 14 Jul 2020 09:48:52: 9000000 INFO @ Tue, 14 Jul 2020 09:48:57: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:48:57: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:48:57: #1 total tags in treatment: 14776146 INFO @ Tue, 14 Jul 2020 09:48:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:48:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:48:58: #1 tags after filtering in treatment: 14776057 INFO @ Tue, 14 Jul 2020 09:48:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:48:58: #1 finished! INFO @ Tue, 14 Jul 2020 09:48:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:48:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:48:58: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 09:48:58: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:48:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:48:59: 10000000 INFO @ Tue, 14 Jul 2020 09:49:06: 11000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:49:13: 12000000 INFO @ Tue, 14 Jul 2020 09:49:20: 13000000 INFO @ Tue, 14 Jul 2020 09:49:26: 14000000 INFO @ Tue, 14 Jul 2020 09:49:32: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:49:32: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:49:32: #1 total tags in treatment: 14776146 INFO @ Tue, 14 Jul 2020 09:49:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:49:32: #1 tags after filtering in treatment: 14776057 INFO @ Tue, 14 Jul 2020 09:49:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:49:32: #1 finished! INFO @ Tue, 14 Jul 2020 09:49:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:49:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:49:33: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 09:49:33: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:49:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780039/SRX7780039.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling