Job ID = 6627031 SRX = SRX7780031 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:37 14563567 reads; of these: 14563567 (100.00%) were unpaired; of these: 727560 (5.00%) aligned 0 times 11399792 (78.28%) aligned exactly 1 time 2436215 (16.73%) aligned >1 times 95.00% overall alignment rate Time searching: 00:03:37 Overall time: 00:03:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2556838 / 13836007 = 0.1848 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:38:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:38:14: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:38:14: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:38:19: 1000000 INFO @ Tue, 14 Jul 2020 09:38:25: 2000000 INFO @ Tue, 14 Jul 2020 09:38:31: 3000000 INFO @ Tue, 14 Jul 2020 09:38:36: 4000000 INFO @ Tue, 14 Jul 2020 09:38:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:38:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:38:44: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:38:44: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:38:48: 6000000 INFO @ Tue, 14 Jul 2020 09:38:51: 1000000 INFO @ Tue, 14 Jul 2020 09:38:55: 7000000 INFO @ Tue, 14 Jul 2020 09:38:58: 2000000 INFO @ Tue, 14 Jul 2020 09:39:01: 8000000 INFO @ Tue, 14 Jul 2020 09:39:05: 3000000 INFO @ Tue, 14 Jul 2020 09:39:08: 9000000 INFO @ Tue, 14 Jul 2020 09:39:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:39:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:39:14: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:39:14: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:39:15: 10000000 INFO @ Tue, 14 Jul 2020 09:39:18: 5000000 INFO @ Tue, 14 Jul 2020 09:39:21: 1000000 INFO @ Tue, 14 Jul 2020 09:39:22: 11000000 INFO @ Tue, 14 Jul 2020 09:39:24: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:39:24: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:39:24: #1 total tags in treatment: 11279169 INFO @ Tue, 14 Jul 2020 09:39:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:39:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:39:24: #1 tags after filtering in treatment: 11279080 INFO @ Tue, 14 Jul 2020 09:39:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:39:24: #1 finished! INFO @ Tue, 14 Jul 2020 09:39:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:39:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:39:25: 6000000 INFO @ Tue, 14 Jul 2020 09:39:25: #2 number of paired peaks: 29 WARNING @ Tue, 14 Jul 2020 09:39:25: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:39:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:39:27: 2000000 INFO @ Tue, 14 Jul 2020 09:39:31: 7000000 INFO @ Tue, 14 Jul 2020 09:39:35: 3000000 INFO @ Tue, 14 Jul 2020 09:39:38: 8000000 INFO @ Tue, 14 Jul 2020 09:39:42: 4000000 INFO @ Tue, 14 Jul 2020 09:39:44: 9000000 INFO @ Tue, 14 Jul 2020 09:39:48: 5000000 INFO @ Tue, 14 Jul 2020 09:39:51: 10000000 INFO @ Tue, 14 Jul 2020 09:39:55: 6000000 INFO @ Tue, 14 Jul 2020 09:39:58: 11000000 INFO @ Tue, 14 Jul 2020 09:40:00: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:40:00: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:40:00: #1 total tags in treatment: 11279169 INFO @ Tue, 14 Jul 2020 09:40:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:40:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:40:00: #1 tags after filtering in treatment: 11279080 INFO @ Tue, 14 Jul 2020 09:40:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:40:00: #1 finished! INFO @ Tue, 14 Jul 2020 09:40:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:40:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:40:01: #2 number of paired peaks: 29 WARNING @ Tue, 14 Jul 2020 09:40:01: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:40:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:40:01: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:40:07: 8000000 INFO @ Tue, 14 Jul 2020 09:40:13: 9000000 INFO @ Tue, 14 Jul 2020 09:40:19: 10000000 INFO @ Tue, 14 Jul 2020 09:40:25: 11000000 INFO @ Tue, 14 Jul 2020 09:40:27: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:40:27: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:40:27: #1 total tags in treatment: 11279169 INFO @ Tue, 14 Jul 2020 09:40:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:40:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:40:27: #1 tags after filtering in treatment: 11279080 INFO @ Tue, 14 Jul 2020 09:40:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:40:27: #1 finished! INFO @ Tue, 14 Jul 2020 09:40:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:40:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:40:28: #2 number of paired peaks: 29 WARNING @ Tue, 14 Jul 2020 09:40:28: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:40:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7780031/SRX7780031.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。