Job ID = 10166866 SRX = SRX7723721 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:26 83374811 reads; of these: 83374811 (100.00%) were unpaired; of these: 5451511 (6.54%) aligned 0 times 65952161 (79.10%) aligned exactly 1 time 11971139 (14.36%) aligned >1 times 93.46% overall alignment rate Time searching: 00:26:26 Overall time: 00:26:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 14353568 / 77923300 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 23:05:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 23:05:48: #1 read tag files... INFO @ Thu, 08 Oct 2020 23:05:48: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 23:05:53: 1000000 INFO @ Thu, 08 Oct 2020 23:05:59: 2000000 INFO @ Thu, 08 Oct 2020 23:06:04: 3000000 INFO @ Thu, 08 Oct 2020 23:06:10: 4000000 INFO @ Thu, 08 Oct 2020 23:06:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 23:06:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 23:06:18: #1 read tag files... INFO @ Thu, 08 Oct 2020 23:06:18: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 23:06:21: 6000000 INFO @ Thu, 08 Oct 2020 23:06:24: 1000000 INFO @ Thu, 08 Oct 2020 23:06:27: 7000000 INFO @ Thu, 08 Oct 2020 23:06:30: 2000000 INFO @ Thu, 08 Oct 2020 23:06:33: 8000000 INFO @ Thu, 08 Oct 2020 23:06:36: 3000000 INFO @ Thu, 08 Oct 2020 23:06:39: 9000000 INFO @ Thu, 08 Oct 2020 23:06:42: 4000000 INFO @ Thu, 08 Oct 2020 23:06:45: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 23:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 23:06:48: #1 read tag files... INFO @ Thu, 08 Oct 2020 23:06:48: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 23:06:48: 5000000 INFO @ Thu, 08 Oct 2020 23:06:52: 11000000 INFO @ Thu, 08 Oct 2020 23:06:54: 1000000 INFO @ Thu, 08 Oct 2020 23:06:54: 6000000 INFO @ Thu, 08 Oct 2020 23:06:58: 12000000 INFO @ Thu, 08 Oct 2020 23:07:00: 2000000 INFO @ Thu, 08 Oct 2020 23:07:00: 7000000 INFO @ Thu, 08 Oct 2020 23:07:04: 13000000 INFO @ Thu, 08 Oct 2020 23:07:06: 3000000 INFO @ Thu, 08 Oct 2020 23:07:06: 8000000 INFO @ Thu, 08 Oct 2020 23:07:10: 14000000 INFO @ Thu, 08 Oct 2020 23:07:12: 4000000 INFO @ Thu, 08 Oct 2020 23:07:13: 9000000 INFO @ Thu, 08 Oct 2020 23:07:16: 15000000 INFO @ Thu, 08 Oct 2020 23:07:18: 5000000 INFO @ Thu, 08 Oct 2020 23:07:19: 10000000 INFO @ Thu, 08 Oct 2020 23:07:22: 16000000 INFO @ Thu, 08 Oct 2020 23:07:25: 6000000 INFO @ Thu, 08 Oct 2020 23:07:25: 11000000 INFO @ Thu, 08 Oct 2020 23:07:28: 17000000 INFO @ Thu, 08 Oct 2020 23:07:31: 7000000 INFO @ Thu, 08 Oct 2020 23:07:31: 12000000 INFO @ Thu, 08 Oct 2020 23:07:34: 18000000 INFO @ Thu, 08 Oct 2020 23:07:37: 8000000 INFO @ Thu, 08 Oct 2020 23:07:37: 13000000 INFO @ Thu, 08 Oct 2020 23:07:40: 19000000 INFO @ Thu, 08 Oct 2020 23:07:43: 9000000 INFO @ Thu, 08 Oct 2020 23:07:43: 14000000 INFO @ Thu, 08 Oct 2020 23:07:47: 20000000 INFO @ Thu, 08 Oct 2020 23:07:49: 10000000 INFO @ Thu, 08 Oct 2020 23:07:49: 15000000 INFO @ Thu, 08 Oct 2020 23:07:53: 21000000 INFO @ Thu, 08 Oct 2020 23:07:55: 11000000 INFO @ Thu, 08 Oct 2020 23:07:55: 16000000 INFO @ Thu, 08 Oct 2020 23:07:59: 22000000 INFO @ Thu, 08 Oct 2020 23:08:01: 12000000 INFO @ Thu, 08 Oct 2020 23:08:02: 17000000 INFO @ Thu, 08 Oct 2020 23:08:05: 23000000 INFO @ Thu, 08 Oct 2020 23:08:07: 13000000 INFO @ Thu, 08 Oct 2020 23:08:08: 18000000 INFO @ Thu, 08 Oct 2020 23:08:11: 24000000 INFO @ Thu, 08 Oct 2020 23:08:14: 14000000 INFO @ Thu, 08 Oct 2020 23:08:14: 19000000 INFO @ Thu, 08 Oct 2020 23:08:17: 25000000 INFO @ Thu, 08 Oct 2020 23:08:20: 15000000 INFO @ Thu, 08 Oct 2020 23:08:20: 20000000 INFO @ Thu, 08 Oct 2020 23:08:23: 26000000 INFO @ Thu, 08 Oct 2020 23:08:26: 16000000 INFO @ Thu, 08 Oct 2020 23:08:26: 21000000 INFO @ Thu, 08 Oct 2020 23:08:29: 27000000 INFO @ Thu, 08 Oct 2020 23:08:32: 17000000 INFO @ Thu, 08 Oct 2020 23:08:32: 22000000 INFO @ Thu, 08 Oct 2020 23:08:35: 28000000 INFO @ Thu, 08 Oct 2020 23:08:38: 23000000 INFO @ Thu, 08 Oct 2020 23:08:38: 18000000 INFO @ Thu, 08 Oct 2020 23:08:41: 29000000 INFO @ Thu, 08 Oct 2020 23:08:44: 24000000 INFO @ Thu, 08 Oct 2020 23:08:44: 19000000 INFO @ Thu, 08 Oct 2020 23:08:47: 30000000 INFO @ Thu, 08 Oct 2020 23:08:50: 25000000 INFO @ Thu, 08 Oct 2020 23:08:50: 20000000 INFO @ Thu, 08 Oct 2020 23:08:53: 31000000 INFO @ Thu, 08 Oct 2020 23:08:56: 26000000 INFO @ Thu, 08 Oct 2020 23:08:56: 21000000 INFO @ Thu, 08 Oct 2020 23:08:59: 32000000 INFO @ Thu, 08 Oct 2020 23:09:02: 27000000 INFO @ Thu, 08 Oct 2020 23:09:02: 22000000 INFO @ Thu, 08 Oct 2020 23:09:05: 33000000 INFO @ Thu, 08 Oct 2020 23:09:08: 28000000 INFO @ Thu, 08 Oct 2020 23:09:08: 23000000 INFO @ Thu, 08 Oct 2020 23:09:11: 34000000 INFO @ Thu, 08 Oct 2020 23:09:14: 29000000 INFO @ Thu, 08 Oct 2020 23:09:14: 24000000 INFO @ Thu, 08 Oct 2020 23:09:17: 35000000 INFO @ Thu, 08 Oct 2020 23:09:20: 30000000 INFO @ Thu, 08 Oct 2020 23:09:20: 25000000 INFO @ Thu, 08 Oct 2020 23:09:24: 36000000 INFO @ Thu, 08 Oct 2020 23:09:26: 31000000 INFO @ Thu, 08 Oct 2020 23:09:26: 26000000 INFO @ Thu, 08 Oct 2020 23:09:30: 37000000 INFO @ Thu, 08 Oct 2020 23:09:32: 32000000 INFO @ Thu, 08 Oct 2020 23:09:32: 27000000 INFO @ Thu, 08 Oct 2020 23:09:36: 38000000 INFO @ Thu, 08 Oct 2020 23:09:38: 33000000 INFO @ Thu, 08 Oct 2020 23:09:39: 28000000 INFO @ Thu, 08 Oct 2020 23:09:42: 39000000 INFO @ Thu, 08 Oct 2020 23:09:44: 34000000 INFO @ Thu, 08 Oct 2020 23:09:45: 29000000 INFO @ Thu, 08 Oct 2020 23:09:48: 40000000 INFO @ Thu, 08 Oct 2020 23:09:50: 35000000 INFO @ Thu, 08 Oct 2020 23:09:51: 30000000 INFO @ Thu, 08 Oct 2020 23:09:54: 41000000 INFO @ Thu, 08 Oct 2020 23:09:56: 36000000 INFO @ Thu, 08 Oct 2020 23:09:57: 31000000 INFO @ Thu, 08 Oct 2020 23:10:00: 42000000 INFO @ Thu, 08 Oct 2020 23:10:02: 37000000 INFO @ Thu, 08 Oct 2020 23:10:03: 32000000 INFO @ Thu, 08 Oct 2020 23:10:06: 43000000 INFO @ Thu, 08 Oct 2020 23:10:08: 38000000 INFO @ Thu, 08 Oct 2020 23:10:09: 33000000 INFO @ Thu, 08 Oct 2020 23:10:12: 44000000 INFO @ Thu, 08 Oct 2020 23:10:14: 39000000 INFO @ Thu, 08 Oct 2020 23:10:15: 34000000 INFO @ Thu, 08 Oct 2020 23:10:18: 45000000 INFO @ Thu, 08 Oct 2020 23:10:20: 40000000 INFO @ Thu, 08 Oct 2020 23:10:21: 35000000 INFO @ Thu, 08 Oct 2020 23:10:24: 46000000 INFO @ Thu, 08 Oct 2020 23:10:26: 41000000 INFO @ Thu, 08 Oct 2020 23:10:27: 36000000 INFO @ Thu, 08 Oct 2020 23:10:30: 47000000 INFO @ Thu, 08 Oct 2020 23:10:32: 42000000 INFO @ Thu, 08 Oct 2020 23:10:33: 37000000 INFO @ Thu, 08 Oct 2020 23:10:36: 48000000 INFO @ Thu, 08 Oct 2020 23:10:38: 43000000 INFO @ Thu, 08 Oct 2020 23:10:39: 38000000 INFO @ Thu, 08 Oct 2020 23:10:42: 49000000 INFO @ Thu, 08 Oct 2020 23:10:44: 44000000 INFO @ Thu, 08 Oct 2020 23:10:45: 39000000 INFO @ Thu, 08 Oct 2020 23:10:48: 50000000 INFO @ Thu, 08 Oct 2020 23:10:51: 45000000 INFO @ Thu, 08 Oct 2020 23:10:51: 40000000 INFO @ Thu, 08 Oct 2020 23:10:54: 51000000 INFO @ Thu, 08 Oct 2020 23:10:57: 46000000 INFO @ Thu, 08 Oct 2020 23:10:57: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 23:11:01: 52000000 INFO @ Thu, 08 Oct 2020 23:11:03: 47000000 INFO @ Thu, 08 Oct 2020 23:11:03: 42000000 INFO @ Thu, 08 Oct 2020 23:11:07: 53000000 INFO @ Thu, 08 Oct 2020 23:11:09: 48000000 INFO @ Thu, 08 Oct 2020 23:11:09: 43000000 INFO @ Thu, 08 Oct 2020 23:11:13: 54000000 INFO @ Thu, 08 Oct 2020 23:11:15: 49000000 INFO @ Thu, 08 Oct 2020 23:11:15: 44000000 INFO @ Thu, 08 Oct 2020 23:11:19: 55000000 INFO @ Thu, 08 Oct 2020 23:11:21: 50000000 INFO @ Thu, 08 Oct 2020 23:11:21: 45000000 INFO @ Thu, 08 Oct 2020 23:11:25: 56000000 INFO @ Thu, 08 Oct 2020 23:11:27: 51000000 INFO @ Thu, 08 Oct 2020 23:11:27: 46000000 INFO @ Thu, 08 Oct 2020 23:11:31: 57000000 INFO @ Thu, 08 Oct 2020 23:11:33: 52000000 INFO @ Thu, 08 Oct 2020 23:11:33: 47000000 INFO @ Thu, 08 Oct 2020 23:11:37: 58000000 INFO @ Thu, 08 Oct 2020 23:11:39: 53000000 INFO @ Thu, 08 Oct 2020 23:11:39: 48000000 INFO @ Thu, 08 Oct 2020 23:11:43: 59000000 INFO @ Thu, 08 Oct 2020 23:11:45: 54000000 INFO @ Thu, 08 Oct 2020 23:11:46: 49000000 INFO @ Thu, 08 Oct 2020 23:11:49: 60000000 INFO @ Thu, 08 Oct 2020 23:11:51: 55000000 INFO @ Thu, 08 Oct 2020 23:11:52: 50000000 INFO @ Thu, 08 Oct 2020 23:11:55: 61000000 INFO @ Thu, 08 Oct 2020 23:11:57: 56000000 INFO @ Thu, 08 Oct 2020 23:11:58: 51000000 INFO @ Thu, 08 Oct 2020 23:12:02: 62000000 INFO @ Thu, 08 Oct 2020 23:12:04: 57000000 INFO @ Thu, 08 Oct 2020 23:12:04: 52000000 INFO @ Thu, 08 Oct 2020 23:12:08: 63000000 INFO @ Thu, 08 Oct 2020 23:12:11: 53000000 INFO @ Thu, 08 Oct 2020 23:12:11: 58000000 INFO @ Thu, 08 Oct 2020 23:12:13: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 23:12:13: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 23:12:13: #1 total tags in treatment: 63569732 INFO @ Thu, 08 Oct 2020 23:12:13: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 23:12:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 23:12:14: #1 tags after filtering in treatment: 63569683 INFO @ Thu, 08 Oct 2020 23:12:14: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 23:12:14: #1 finished! INFO @ Thu, 08 Oct 2020 23:12:14: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 23:12:14: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 23:12:17: 54000000 INFO @ Thu, 08 Oct 2020 23:12:17: 59000000 INFO @ Thu, 08 Oct 2020 23:12:18: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 23:12:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 23:12:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 23:12:23: 55000000 INFO @ Thu, 08 Oct 2020 23:12:23: 60000000 INFO @ Thu, 08 Oct 2020 23:12:29: 56000000 INFO @ Thu, 08 Oct 2020 23:12:29: 61000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 23:12:36: 57000000 INFO @ Thu, 08 Oct 2020 23:12:36: 62000000 INFO @ Thu, 08 Oct 2020 23:12:42: 58000000 INFO @ Thu, 08 Oct 2020 23:12:42: 63000000 INFO @ Thu, 08 Oct 2020 23:12:46: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 23:12:46: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 23:12:46: #1 total tags in treatment: 63569732 INFO @ Thu, 08 Oct 2020 23:12:46: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 23:12:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 23:12:47: #1 tags after filtering in treatment: 63569683 INFO @ Thu, 08 Oct 2020 23:12:47: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 23:12:47: #1 finished! INFO @ Thu, 08 Oct 2020 23:12:47: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 23:12:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 23:12:48: 59000000 INFO @ Thu, 08 Oct 2020 23:12:51: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 23:12:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 23:12:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 23:12:54: 60000000 INFO @ Thu, 08 Oct 2020 23:13:00: 61000000 INFO @ Thu, 08 Oct 2020 23:13:06: 62000000 INFO @ Thu, 08 Oct 2020 23:13:12: 63000000 INFO @ Thu, 08 Oct 2020 23:13:15: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 23:13:15: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 23:13:15: #1 total tags in treatment: 63569732 INFO @ Thu, 08 Oct 2020 23:13:15: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 23:13:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 23:13:17: #1 tags after filtering in treatment: 63569683 INFO @ Thu, 08 Oct 2020 23:13:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 23:13:17: #1 finished! INFO @ Thu, 08 Oct 2020 23:13:17: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 23:13:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 23:13:20: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 23:13:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 23:13:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling