Job ID = 14167388 SRX = SRX7723721 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:42 83374811 reads; of these: 83374811 (100.00%) were unpaired; of these: 5451511 (6.54%) aligned 0 times 65952161 (79.10%) aligned exactly 1 time 11971139 (14.36%) aligned >1 times 93.46% overall alignment rate Time searching: 00:26:42 Overall time: 00:26:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 14353568 / 77923300 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:49:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:49:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:49:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:49:48: 1000000 INFO @ Fri, 10 Dec 2021 12:49:53: 2000000 INFO @ Fri, 10 Dec 2021 12:49:59: 3000000 INFO @ Fri, 10 Dec 2021 12:50:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:50:10: 5000000 INFO @ Fri, 10 Dec 2021 12:50:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:50:12: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:50:12: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:50:16: 6000000 INFO @ Fri, 10 Dec 2021 12:50:18: 1000000 INFO @ Fri, 10 Dec 2021 12:50:23: 7000000 INFO @ Fri, 10 Dec 2021 12:50:25: 2000000 INFO @ Fri, 10 Dec 2021 12:50:29: 8000000 INFO @ Fri, 10 Dec 2021 12:50:31: 3000000 INFO @ Fri, 10 Dec 2021 12:50:36: 9000000 INFO @ Fri, 10 Dec 2021 12:50:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:50:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:50:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:50:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:50:42: 10000000 INFO @ Fri, 10 Dec 2021 12:50:44: 5000000 INFO @ Fri, 10 Dec 2021 12:50:48: 1000000 INFO @ Fri, 10 Dec 2021 12:50:48: 11000000 INFO @ Fri, 10 Dec 2021 12:50:50: 6000000 INFO @ Fri, 10 Dec 2021 12:50:55: 2000000 INFO @ Fri, 10 Dec 2021 12:50:55: 12000000 INFO @ Fri, 10 Dec 2021 12:50:57: 7000000 INFO @ Fri, 10 Dec 2021 12:51:01: 3000000 INFO @ Fri, 10 Dec 2021 12:51:02: 13000000 INFO @ Fri, 10 Dec 2021 12:51:04: 8000000 INFO @ Fri, 10 Dec 2021 12:51:08: 4000000 INFO @ Fri, 10 Dec 2021 12:51:08: 14000000 INFO @ Fri, 10 Dec 2021 12:51:10: 9000000 INFO @ Fri, 10 Dec 2021 12:51:14: 5000000 INFO @ Fri, 10 Dec 2021 12:51:15: 15000000 INFO @ Fri, 10 Dec 2021 12:51:17: 10000000 INFO @ Fri, 10 Dec 2021 12:51:21: 6000000 INFO @ Fri, 10 Dec 2021 12:51:21: 16000000 INFO @ Fri, 10 Dec 2021 12:51:23: 11000000 INFO @ Fri, 10 Dec 2021 12:51:27: 7000000 INFO @ Fri, 10 Dec 2021 12:51:28: 17000000 INFO @ Fri, 10 Dec 2021 12:51:30: 12000000 INFO @ Fri, 10 Dec 2021 12:51:34: 8000000 INFO @ Fri, 10 Dec 2021 12:51:34: 18000000 INFO @ Fri, 10 Dec 2021 12:51:36: 13000000 INFO @ Fri, 10 Dec 2021 12:51:40: 9000000 INFO @ Fri, 10 Dec 2021 12:51:41: 19000000 INFO @ Fri, 10 Dec 2021 12:51:43: 14000000 INFO @ Fri, 10 Dec 2021 12:51:47: 10000000 INFO @ Fri, 10 Dec 2021 12:51:47: 20000000 INFO @ Fri, 10 Dec 2021 12:51:49: 15000000 INFO @ Fri, 10 Dec 2021 12:51:53: 11000000 INFO @ Fri, 10 Dec 2021 12:51:54: 21000000 INFO @ Fri, 10 Dec 2021 12:51:56: 16000000 INFO @ Fri, 10 Dec 2021 12:52:00: 12000000 INFO @ Fri, 10 Dec 2021 12:52:00: 22000000 INFO @ Fri, 10 Dec 2021 12:52:02: 17000000 INFO @ Fri, 10 Dec 2021 12:52:06: 13000000 INFO @ Fri, 10 Dec 2021 12:52:07: 23000000 INFO @ Fri, 10 Dec 2021 12:52:09: 18000000 INFO @ Fri, 10 Dec 2021 12:52:12: 14000000 INFO @ Fri, 10 Dec 2021 12:52:14: 24000000 INFO @ Fri, 10 Dec 2021 12:52:15: 19000000 INFO @ Fri, 10 Dec 2021 12:52:19: 15000000 INFO @ Fri, 10 Dec 2021 12:52:20: 25000000 INFO @ Fri, 10 Dec 2021 12:52:22: 20000000 INFO @ Fri, 10 Dec 2021 12:52:25: 16000000 INFO @ Fri, 10 Dec 2021 12:52:27: 26000000 INFO @ Fri, 10 Dec 2021 12:52:29: 21000000 INFO @ Fri, 10 Dec 2021 12:52:32: 17000000 INFO @ Fri, 10 Dec 2021 12:52:33: 27000000 INFO @ Fri, 10 Dec 2021 12:52:35: 22000000 INFO @ Fri, 10 Dec 2021 12:52:38: 18000000 INFO @ Fri, 10 Dec 2021 12:52:40: 28000000 INFO @ Fri, 10 Dec 2021 12:52:42: 23000000 INFO @ Fri, 10 Dec 2021 12:52:45: 19000000 INFO @ Fri, 10 Dec 2021 12:52:46: 29000000 INFO @ Fri, 10 Dec 2021 12:52:48: 24000000 INFO @ Fri, 10 Dec 2021 12:52:51: 20000000 INFO @ Fri, 10 Dec 2021 12:52:53: 30000000 INFO @ Fri, 10 Dec 2021 12:52:55: 25000000 INFO @ Fri, 10 Dec 2021 12:52:57: 21000000 INFO @ Fri, 10 Dec 2021 12:52:59: 31000000 INFO @ Fri, 10 Dec 2021 12:53:01: 26000000 INFO @ Fri, 10 Dec 2021 12:53:04: 22000000 INFO @ Fri, 10 Dec 2021 12:53:06: 32000000 INFO @ Fri, 10 Dec 2021 12:53:08: 27000000 INFO @ Fri, 10 Dec 2021 12:53:10: 23000000 INFO @ Fri, 10 Dec 2021 12:53:12: 33000000 INFO @ Fri, 10 Dec 2021 12:53:14: 28000000 INFO @ Fri, 10 Dec 2021 12:53:17: 24000000 INFO @ Fri, 10 Dec 2021 12:53:19: 34000000 INFO @ Fri, 10 Dec 2021 12:53:21: 29000000 INFO @ Fri, 10 Dec 2021 12:53:23: 25000000 INFO @ Fri, 10 Dec 2021 12:53:25: 35000000 INFO @ Fri, 10 Dec 2021 12:53:27: 30000000 INFO @ Fri, 10 Dec 2021 12:53:30: 26000000 INFO @ Fri, 10 Dec 2021 12:53:32: 36000000 INFO @ Fri, 10 Dec 2021 12:53:34: 31000000 INFO @ Fri, 10 Dec 2021 12:53:36: 27000000 INFO @ Fri, 10 Dec 2021 12:53:38: 37000000 INFO @ Fri, 10 Dec 2021 12:53:40: 32000000 INFO @ Fri, 10 Dec 2021 12:53:42: 28000000 INFO @ Fri, 10 Dec 2021 12:53:45: 38000000 INFO @ Fri, 10 Dec 2021 12:53:47: 33000000 INFO @ Fri, 10 Dec 2021 12:53:49: 29000000 INFO @ Fri, 10 Dec 2021 12:53:51: 39000000 INFO @ Fri, 10 Dec 2021 12:53:53: 34000000 INFO @ Fri, 10 Dec 2021 12:53:55: 30000000 INFO @ Fri, 10 Dec 2021 12:53:58: 40000000 INFO @ Fri, 10 Dec 2021 12:54:00: 35000000 INFO @ Fri, 10 Dec 2021 12:54:02: 31000000 INFO @ Fri, 10 Dec 2021 12:54:04: 41000000 INFO @ Fri, 10 Dec 2021 12:54:06: 36000000 INFO @ Fri, 10 Dec 2021 12:54:08: 32000000 INFO @ Fri, 10 Dec 2021 12:54:11: 42000000 INFO @ Fri, 10 Dec 2021 12:54:13: 37000000 INFO @ Fri, 10 Dec 2021 12:54:15: 33000000 INFO @ Fri, 10 Dec 2021 12:54:17: 43000000 INFO @ Fri, 10 Dec 2021 12:54:19: 38000000 INFO @ Fri, 10 Dec 2021 12:54:21: 34000000 INFO @ Fri, 10 Dec 2021 12:54:24: 44000000 INFO @ Fri, 10 Dec 2021 12:54:26: 39000000 INFO @ Fri, 10 Dec 2021 12:54:27: 35000000 INFO @ Fri, 10 Dec 2021 12:54:31: 45000000 INFO @ Fri, 10 Dec 2021 12:54:32: 40000000 INFO @ Fri, 10 Dec 2021 12:54:34: 36000000 INFO @ Fri, 10 Dec 2021 12:54:37: 46000000 INFO @ Fri, 10 Dec 2021 12:54:39: 41000000 INFO @ Fri, 10 Dec 2021 12:54:40: 37000000 INFO @ Fri, 10 Dec 2021 12:54:44: 47000000 INFO @ Fri, 10 Dec 2021 12:54:45: 42000000 INFO @ Fri, 10 Dec 2021 12:54:47: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:54:50: 48000000 INFO @ Fri, 10 Dec 2021 12:54:52: 43000000 INFO @ Fri, 10 Dec 2021 12:54:53: 39000000 INFO @ Fri, 10 Dec 2021 12:54:57: 49000000 INFO @ Fri, 10 Dec 2021 12:54:59: 44000000 INFO @ Fri, 10 Dec 2021 12:55:00: 40000000 INFO @ Fri, 10 Dec 2021 12:55:03: 50000000 INFO @ Fri, 10 Dec 2021 12:55:05: 45000000 INFO @ Fri, 10 Dec 2021 12:55:06: 41000000 INFO @ Fri, 10 Dec 2021 12:55:10: 51000000 INFO @ Fri, 10 Dec 2021 12:55:12: 46000000 INFO @ Fri, 10 Dec 2021 12:55:12: 42000000 INFO @ Fri, 10 Dec 2021 12:55:17: 52000000 INFO @ Fri, 10 Dec 2021 12:55:18: 47000000 INFO @ Fri, 10 Dec 2021 12:55:19: 43000000 INFO @ Fri, 10 Dec 2021 12:55:23: 53000000 INFO @ Fri, 10 Dec 2021 12:55:25: 48000000 INFO @ Fri, 10 Dec 2021 12:55:25: 44000000 INFO @ Fri, 10 Dec 2021 12:55:30: 54000000 INFO @ Fri, 10 Dec 2021 12:55:31: 49000000 INFO @ Fri, 10 Dec 2021 12:55:32: 45000000 INFO @ Fri, 10 Dec 2021 12:55:36: 55000000 INFO @ Fri, 10 Dec 2021 12:55:38: 50000000 INFO @ Fri, 10 Dec 2021 12:55:38: 46000000 INFO @ Fri, 10 Dec 2021 12:55:43: 56000000 INFO @ Fri, 10 Dec 2021 12:55:44: 51000000 INFO @ Fri, 10 Dec 2021 12:55:44: 47000000 INFO @ Fri, 10 Dec 2021 12:55:49: 57000000 INFO @ Fri, 10 Dec 2021 12:55:51: 48000000 INFO @ Fri, 10 Dec 2021 12:55:51: 52000000 INFO @ Fri, 10 Dec 2021 12:55:56: 58000000 INFO @ Fri, 10 Dec 2021 12:55:57: 49000000 INFO @ Fri, 10 Dec 2021 12:55:57: 53000000 INFO @ Fri, 10 Dec 2021 12:56:02: 59000000 INFO @ Fri, 10 Dec 2021 12:56:04: 50000000 INFO @ Fri, 10 Dec 2021 12:56:04: 54000000 INFO @ Fri, 10 Dec 2021 12:56:08: 60000000 INFO @ Fri, 10 Dec 2021 12:56:10: 51000000 INFO @ Fri, 10 Dec 2021 12:56:10: 55000000 INFO @ Fri, 10 Dec 2021 12:56:15: 61000000 INFO @ Fri, 10 Dec 2021 12:56:17: 56000000 INFO @ Fri, 10 Dec 2021 12:56:17: 52000000 INFO @ Fri, 10 Dec 2021 12:56:21: 62000000 INFO @ Fri, 10 Dec 2021 12:56:23: 57000000 INFO @ Fri, 10 Dec 2021 12:56:23: 53000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:56:28: 63000000 INFO @ Fri, 10 Dec 2021 12:56:29: 58000000 INFO @ Fri, 10 Dec 2021 12:56:30: 54000000 INFO @ Fri, 10 Dec 2021 12:56:32: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 12:56:32: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 12:56:32: #1 total tags in treatment: 63569732 INFO @ Fri, 10 Dec 2021 12:56:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:56:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:56:33: #1 tags after filtering in treatment: 63569683 INFO @ Fri, 10 Dec 2021 12:56:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:56:33: #1 finished! INFO @ Fri, 10 Dec 2021 12:56:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:56:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:56:36: 59000000 INFO @ Fri, 10 Dec 2021 12:56:36: 55000000 INFO @ Fri, 10 Dec 2021 12:56:37: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:56:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:56:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:56:42: 60000000 INFO @ Fri, 10 Dec 2021 12:56:43: 56000000 INFO @ Fri, 10 Dec 2021 12:56:48: 61000000 INFO @ Fri, 10 Dec 2021 12:56:49: 57000000 INFO @ Fri, 10 Dec 2021 12:56:55: 62000000 INFO @ Fri, 10 Dec 2021 12:56:55: 58000000 INFO @ Fri, 10 Dec 2021 12:57:01: 63000000 INFO @ Fri, 10 Dec 2021 12:57:02: 59000000 INFO @ Fri, 10 Dec 2021 12:57:05: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 12:57:05: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 12:57:05: #1 total tags in treatment: 63569732 INFO @ Fri, 10 Dec 2021 12:57:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:57:07: #1 tags after filtering in treatment: 63569683 INFO @ Fri, 10 Dec 2021 12:57:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:57:07: #1 finished! INFO @ Fri, 10 Dec 2021 12:57:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:57:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:57:08: 60000000 INFO @ Fri, 10 Dec 2021 12:57:10: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:57:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:57:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:57:14: 61000000 INFO @ Fri, 10 Dec 2021 12:57:20: 62000000 INFO @ Fri, 10 Dec 2021 12:57:26: 63000000 INFO @ Fri, 10 Dec 2021 12:57:30: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 12:57:30: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 12:57:30: #1 total tags in treatment: 63569732 INFO @ Fri, 10 Dec 2021 12:57:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:57:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:57:31: #1 tags after filtering in treatment: 63569683 INFO @ Fri, 10 Dec 2021 12:57:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:57:31: #1 finished! INFO @ Fri, 10 Dec 2021 12:57:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:57:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:57:35: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:57:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:57:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723721/SRX7723721.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling