Job ID = 14167297 SRX = SRX7723706 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:31 25541089 reads; of these: 25541089 (100.00%) were unpaired; of these: 5255761 (20.58%) aligned 0 times 17421315 (68.21%) aligned exactly 1 time 2864013 (11.21%) aligned >1 times 79.42% overall alignment rate Time searching: 00:07:31 Overall time: 00:07:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1505521 / 20285328 = 0.0742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:56:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:56:02: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:56:02: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:56:10: 1000000 INFO @ Fri, 10 Dec 2021 11:56:18: 2000000 INFO @ Fri, 10 Dec 2021 11:56:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:56:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:56:32: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:56:32: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:56:34: 4000000 INFO @ Fri, 10 Dec 2021 11:56:40: 1000000 INFO @ Fri, 10 Dec 2021 11:56:42: 5000000 INFO @ Fri, 10 Dec 2021 11:56:49: 2000000 INFO @ Fri, 10 Dec 2021 11:56:50: 6000000 INFO @ Fri, 10 Dec 2021 11:56:57: 3000000 INFO @ Fri, 10 Dec 2021 11:56:58: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:57:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:57:02: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:57:02: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:57:05: 4000000 INFO @ Fri, 10 Dec 2021 11:57:06: 8000000 INFO @ Fri, 10 Dec 2021 11:57:10: 1000000 INFO @ Fri, 10 Dec 2021 11:57:13: 5000000 INFO @ Fri, 10 Dec 2021 11:57:15: 9000000 INFO @ Fri, 10 Dec 2021 11:57:18: 2000000 INFO @ Fri, 10 Dec 2021 11:57:21: 6000000 INFO @ Fri, 10 Dec 2021 11:57:23: 10000000 INFO @ Fri, 10 Dec 2021 11:57:26: 3000000 INFO @ Fri, 10 Dec 2021 11:57:30: 7000000 INFO @ Fri, 10 Dec 2021 11:57:31: 11000000 INFO @ Fri, 10 Dec 2021 11:57:33: 4000000 INFO @ Fri, 10 Dec 2021 11:57:38: 8000000 INFO @ Fri, 10 Dec 2021 11:57:39: 12000000 INFO @ Fri, 10 Dec 2021 11:57:41: 5000000 INFO @ Fri, 10 Dec 2021 11:57:46: 9000000 INFO @ Fri, 10 Dec 2021 11:57:47: 13000000 INFO @ Fri, 10 Dec 2021 11:57:49: 6000000 INFO @ Fri, 10 Dec 2021 11:57:54: 10000000 INFO @ Fri, 10 Dec 2021 11:57:55: 14000000 INFO @ Fri, 10 Dec 2021 11:57:57: 7000000 INFO @ Fri, 10 Dec 2021 11:58:03: 11000000 INFO @ Fri, 10 Dec 2021 11:58:03: 15000000 INFO @ Fri, 10 Dec 2021 11:58:04: 8000000 INFO @ Fri, 10 Dec 2021 11:58:11: 12000000 INFO @ Fri, 10 Dec 2021 11:58:12: 16000000 INFO @ Fri, 10 Dec 2021 11:58:12: 9000000 INFO @ Fri, 10 Dec 2021 11:58:19: 13000000 INFO @ Fri, 10 Dec 2021 11:58:20: 10000000 INFO @ Fri, 10 Dec 2021 11:58:20: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:58:27: 14000000 INFO @ Fri, 10 Dec 2021 11:58:28: 11000000 INFO @ Fri, 10 Dec 2021 11:58:28: 18000000 INFO @ Fri, 10 Dec 2021 11:58:35: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 11:58:35: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 11:58:35: #1 total tags in treatment: 18779807 INFO @ Fri, 10 Dec 2021 11:58:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:58:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:58:35: 12000000 INFO @ Fri, 10 Dec 2021 11:58:35: #1 tags after filtering in treatment: 18779622 INFO @ Fri, 10 Dec 2021 11:58:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:58:35: #1 finished! INFO @ Fri, 10 Dec 2021 11:58:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:58:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:58:36: 15000000 INFO @ Fri, 10 Dec 2021 11:58:37: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:58:37: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:58:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:58:43: 13000000 INFO @ Fri, 10 Dec 2021 11:58:44: 16000000 INFO @ Fri, 10 Dec 2021 11:58:51: 14000000 INFO @ Fri, 10 Dec 2021 11:58:52: 17000000 INFO @ Fri, 10 Dec 2021 11:58:58: 15000000 INFO @ Fri, 10 Dec 2021 11:59:01: 18000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:59:06: 16000000 INFO @ Fri, 10 Dec 2021 11:59:07: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 11:59:07: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 11:59:07: #1 total tags in treatment: 18779807 INFO @ Fri, 10 Dec 2021 11:59:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:59:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:59:08: #1 tags after filtering in treatment: 18779622 INFO @ Fri, 10 Dec 2021 11:59:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:59:08: #1 finished! INFO @ Fri, 10 Dec 2021 11:59:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:59:09: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:59:09: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:59:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:59:14: 17000000 INFO @ Fri, 10 Dec 2021 11:59:21: 18000000 INFO @ Fri, 10 Dec 2021 11:59:26: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 11:59:26: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 11:59:26: #1 total tags in treatment: 18779807 INFO @ Fri, 10 Dec 2021 11:59:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:59:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:59:27: #1 tags after filtering in treatment: 18779622 INFO @ Fri, 10 Dec 2021 11:59:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:59:27: #1 finished! INFO @ Fri, 10 Dec 2021 11:59:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:59:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:59:28: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:59:28: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:59:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling