Job ID = 10166541 SRX = SRX7723706 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:39 25541089 reads; of these: 25541089 (100.00%) were unpaired; of these: 5255761 (20.58%) aligned 0 times 17421315 (68.21%) aligned exactly 1 time 2864013 (11.21%) aligned >1 times 79.42% overall alignment rate Time searching: 00:07:40 Overall time: 00:07:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1505521 / 20285328 = 0.0742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:55:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:55:01: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:55:01: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:55:07: 1000000 INFO @ Thu, 08 Oct 2020 21:55:13: 2000000 INFO @ Thu, 08 Oct 2020 21:55:19: 3000000 INFO @ Thu, 08 Oct 2020 21:55:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:55:31: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:55:31: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:55:32: 5000000 INFO @ Thu, 08 Oct 2020 21:55:38: 1000000 INFO @ Thu, 08 Oct 2020 21:55:38: 6000000 INFO @ Thu, 08 Oct 2020 21:55:45: 2000000 INFO @ Thu, 08 Oct 2020 21:55:45: 7000000 INFO @ Thu, 08 Oct 2020 21:55:52: 3000000 INFO @ Thu, 08 Oct 2020 21:55:52: 8000000 BedGraph に変換中... INFO @ Thu, 08 Oct 2020 21:55:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:55:59: 9000000 INFO @ Thu, 08 Oct 2020 21:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:56:01: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:56:01: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:56:06: 5000000 INFO @ Thu, 08 Oct 2020 21:56:06: 10000000 INFO @ Thu, 08 Oct 2020 21:56:08: 1000000 INFO @ Thu, 08 Oct 2020 21:56:13: 11000000 INFO @ Thu, 08 Oct 2020 21:56:13: 6000000 INFO @ Thu, 08 Oct 2020 21:56:15: 2000000 INFO @ Thu, 08 Oct 2020 21:56:20: 12000000 INFO @ Thu, 08 Oct 2020 21:56:20: 7000000 INFO @ Thu, 08 Oct 2020 21:56:22: 3000000 INFO @ Thu, 08 Oct 2020 21:56:27: 13000000 INFO @ Thu, 08 Oct 2020 21:56:28: 8000000 INFO @ Thu, 08 Oct 2020 21:56:29: 4000000 INFO @ Thu, 08 Oct 2020 21:56:34: 14000000 INFO @ Thu, 08 Oct 2020 21:56:35: 9000000 INFO @ Thu, 08 Oct 2020 21:56:37: 5000000 INFO @ Thu, 08 Oct 2020 21:56:41: 15000000 INFO @ Thu, 08 Oct 2020 21:56:42: 10000000 INFO @ Thu, 08 Oct 2020 21:56:44: 6000000 INFO @ Thu, 08 Oct 2020 21:56:49: 16000000 INFO @ Thu, 08 Oct 2020 21:56:49: 11000000 INFO @ Thu, 08 Oct 2020 21:56:51: 7000000 INFO @ Thu, 08 Oct 2020 21:56:56: 17000000 INFO @ Thu, 08 Oct 2020 21:56:57: 12000000 INFO @ Thu, 08 Oct 2020 21:56:58: 8000000 INFO @ Thu, 08 Oct 2020 21:57:03: 18000000 INFO @ Thu, 08 Oct 2020 21:57:04: 13000000 INFO @ Thu, 08 Oct 2020 21:57:05: 9000000 INFO @ Thu, 08 Oct 2020 21:57:08: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:57:08: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:57:08: #1 total tags in treatment: 18779807 INFO @ Thu, 08 Oct 2020 21:57:08: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:57:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:57:09: #1 tags after filtering in treatment: 18779622 INFO @ Thu, 08 Oct 2020 21:57:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:57:09: #1 finished! INFO @ Thu, 08 Oct 2020 21:57:09: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:57:10: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 21:57:10: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:57:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:57:11: 14000000 INFO @ Thu, 08 Oct 2020 21:57:12: 10000000 INFO @ Thu, 08 Oct 2020 21:57:18: 15000000 INFO @ Thu, 08 Oct 2020 21:57:19: 11000000 INFO @ Thu, 08 Oct 2020 21:57:25: 16000000 INFO @ Thu, 08 Oct 2020 21:57:26: 12000000 INFO @ Thu, 08 Oct 2020 21:57:32: 17000000 INFO @ Thu, 08 Oct 2020 21:57:33: 13000000 INFO @ Thu, 08 Oct 2020 21:57:39: 18000000 INFO @ Thu, 08 Oct 2020 21:57:40: 14000000 INFO @ Thu, 08 Oct 2020 21:57:45: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:57:45: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:57:45: #1 total tags in treatment: 18779807 INFO @ Thu, 08 Oct 2020 21:57:45: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:57:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:57:45: #1 tags after filtering in treatment: 18779622 INFO @ Thu, 08 Oct 2020 21:57:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:57:45: #1 finished! INFO @ Thu, 08 Oct 2020 21:57:45: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:57:45: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:57:47: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 21:57:47: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:57:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:57:47: 15000000 INFO @ Thu, 08 Oct 2020 21:57:54: 16000000 INFO @ Thu, 08 Oct 2020 21:58:00: 17000000 INFO @ Thu, 08 Oct 2020 21:58:06: 18000000 INFO @ Thu, 08 Oct 2020 21:58:11: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:58:11: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:58:11: #1 total tags in treatment: 18779807 INFO @ Thu, 08 Oct 2020 21:58:11: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:58:11: #1 tags after filtering in treatment: 18779622 INFO @ Thu, 08 Oct 2020 21:58:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:58:11: #1 finished! INFO @ Thu, 08 Oct 2020 21:58:11: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:58:13: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 21:58:13: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:58:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723706/SRX7723706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。