Job ID = 10166636 SRX = SRX7723703 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:13 51994999 reads; of these: 51994999 (100.00%) were unpaired; of these: 12850127 (24.71%) aligned 0 times 34633763 (66.61%) aligned exactly 1 time 4511109 (8.68%) aligned >1 times 75.29% overall alignment rate Time searching: 00:15:13 Overall time: 00:15:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6742622 / 39144872 = 0.1722 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 22:27:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 22:27:25: #1 read tag files... INFO @ Thu, 08 Oct 2020 22:27:25: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 22:27:32: 1000000 INFO @ Thu, 08 Oct 2020 22:27:40: 2000000 INFO @ Thu, 08 Oct 2020 22:27:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 22:27:54: 4000000 INFO @ Thu, 08 Oct 2020 22:27:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 22:27:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 22:27:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 22:28:02: 5000000 INFO @ Thu, 08 Oct 2020 22:28:05: 1000000 INFO @ Thu, 08 Oct 2020 22:28:10: 6000000 INFO @ Thu, 08 Oct 2020 22:28:13: 2000000 INFO @ Thu, 08 Oct 2020 22:28:18: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 22:28:21: 3000000 INFO @ Thu, 08 Oct 2020 22:28:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 22:28:25: #1 read tag files... INFO @ Thu, 08 Oct 2020 22:28:25: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 22:28:26: 8000000 INFO @ Thu, 08 Oct 2020 22:28:29: 4000000 INFO @ Thu, 08 Oct 2020 22:28:32: 1000000 INFO @ Thu, 08 Oct 2020 22:28:34: 9000000 INFO @ Thu, 08 Oct 2020 22:28:37: 5000000 INFO @ Thu, 08 Oct 2020 22:28:39: 2000000 INFO @ Thu, 08 Oct 2020 22:28:43: 10000000 INFO @ Thu, 08 Oct 2020 22:28:45: 6000000 INFO @ Thu, 08 Oct 2020 22:28:46: 3000000 INFO @ Thu, 08 Oct 2020 22:28:51: 11000000 INFO @ Thu, 08 Oct 2020 22:28:53: 4000000 INFO @ Thu, 08 Oct 2020 22:28:54: 7000000 INFO @ Thu, 08 Oct 2020 22:28:59: 12000000 INFO @ Thu, 08 Oct 2020 22:29:00: 5000000 INFO @ Thu, 08 Oct 2020 22:29:02: 8000000 INFO @ Thu, 08 Oct 2020 22:29:07: 6000000 INFO @ Thu, 08 Oct 2020 22:29:07: 13000000 INFO @ Thu, 08 Oct 2020 22:29:10: 9000000 INFO @ Thu, 08 Oct 2020 22:29:14: 7000000 INFO @ Thu, 08 Oct 2020 22:29:15: 14000000 INFO @ Thu, 08 Oct 2020 22:29:18: 10000000 INFO @ Thu, 08 Oct 2020 22:29:21: 8000000 INFO @ Thu, 08 Oct 2020 22:29:24: 15000000 INFO @ Thu, 08 Oct 2020 22:29:26: 11000000 INFO @ Thu, 08 Oct 2020 22:29:28: 9000000 INFO @ Thu, 08 Oct 2020 22:29:32: 16000000 INFO @ Thu, 08 Oct 2020 22:29:34: 12000000 INFO @ Thu, 08 Oct 2020 22:29:35: 10000000 INFO @ Thu, 08 Oct 2020 22:29:40: 17000000 INFO @ Thu, 08 Oct 2020 22:29:42: 11000000 INFO @ Thu, 08 Oct 2020 22:29:43: 13000000 INFO @ Thu, 08 Oct 2020 22:29:48: 18000000 INFO @ Thu, 08 Oct 2020 22:29:49: 12000000 INFO @ Thu, 08 Oct 2020 22:29:51: 14000000 INFO @ Thu, 08 Oct 2020 22:29:56: 19000000 INFO @ Thu, 08 Oct 2020 22:29:56: 13000000 INFO @ Thu, 08 Oct 2020 22:29:59: 15000000 INFO @ Thu, 08 Oct 2020 22:30:03: 14000000 INFO @ Thu, 08 Oct 2020 22:30:05: 20000000 INFO @ Thu, 08 Oct 2020 22:30:07: 16000000 INFO @ Thu, 08 Oct 2020 22:30:11: 15000000 INFO @ Thu, 08 Oct 2020 22:30:13: 21000000 INFO @ Thu, 08 Oct 2020 22:30:16: 17000000 INFO @ Thu, 08 Oct 2020 22:30:18: 16000000 INFO @ Thu, 08 Oct 2020 22:30:21: 22000000 INFO @ Thu, 08 Oct 2020 22:30:24: 18000000 INFO @ Thu, 08 Oct 2020 22:30:25: 17000000 INFO @ Thu, 08 Oct 2020 22:30:30: 23000000 INFO @ Thu, 08 Oct 2020 22:30:32: 18000000 INFO @ Thu, 08 Oct 2020 22:30:32: 19000000 INFO @ Thu, 08 Oct 2020 22:30:38: 24000000 INFO @ Thu, 08 Oct 2020 22:30:39: 19000000 INFO @ Thu, 08 Oct 2020 22:30:40: 20000000 INFO @ Thu, 08 Oct 2020 22:30:46: 20000000 INFO @ Thu, 08 Oct 2020 22:30:46: 25000000 INFO @ Thu, 08 Oct 2020 22:30:49: 21000000 INFO @ Thu, 08 Oct 2020 22:30:53: 21000000 INFO @ Thu, 08 Oct 2020 22:30:55: 26000000 INFO @ Thu, 08 Oct 2020 22:30:57: 22000000 INFO @ Thu, 08 Oct 2020 22:31:00: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 22:31:04: 27000000 INFO @ Thu, 08 Oct 2020 22:31:05: 23000000 INFO @ Thu, 08 Oct 2020 22:31:07: 23000000 INFO @ Thu, 08 Oct 2020 22:31:12: 28000000 INFO @ Thu, 08 Oct 2020 22:31:13: 24000000 INFO @ Thu, 08 Oct 2020 22:31:14: 24000000 INFO @ Thu, 08 Oct 2020 22:31:20: 29000000 INFO @ Thu, 08 Oct 2020 22:31:22: 25000000 INFO @ Thu, 08 Oct 2020 22:31:22: 25000000 INFO @ Thu, 08 Oct 2020 22:31:28: 30000000 INFO @ Thu, 08 Oct 2020 22:31:31: 26000000 INFO @ Thu, 08 Oct 2020 22:31:31: 26000000 INFO @ Thu, 08 Oct 2020 22:31:36: 31000000 INFO @ Thu, 08 Oct 2020 22:31:40: 27000000 INFO @ Thu, 08 Oct 2020 22:31:40: 27000000 INFO @ Thu, 08 Oct 2020 22:31:44: 32000000 INFO @ Thu, 08 Oct 2020 22:31:48: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:31:48: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:31:48: #1 total tags in treatment: 32402250 INFO @ Thu, 08 Oct 2020 22:31:48: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:31:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:31:48: 28000000 INFO @ Thu, 08 Oct 2020 22:31:48: 28000000 INFO @ Thu, 08 Oct 2020 22:31:48: #1 tags after filtering in treatment: 32402100 INFO @ Thu, 08 Oct 2020 22:31:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 22:31:48: #1 finished! INFO @ Thu, 08 Oct 2020 22:31:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:31:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:31:50: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 22:31:50: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:31:50: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 22:31:56: 29000000 INFO @ Thu, 08 Oct 2020 22:31:57: 29000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 22:32:04: 30000000 cut: /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 22:32:05: 30000000 INFO @ Thu, 08 Oct 2020 22:32:11: 31000000 INFO @ Thu, 08 Oct 2020 22:32:13: 31000000 INFO @ Thu, 08 Oct 2020 22:32:18: 32000000 INFO @ Thu, 08 Oct 2020 22:32:20: 32000000 INFO @ Thu, 08 Oct 2020 22:32:21: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:32:21: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:32:21: #1 total tags in treatment: 32402250 INFO @ Thu, 08 Oct 2020 22:32:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:32:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:32:22: #1 tags after filtering in treatment: 32402100 INFO @ Thu, 08 Oct 2020 22:32:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 22:32:22: #1 finished! INFO @ Thu, 08 Oct 2020 22:32:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:32:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:32:24: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:32:24: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:32:24: #1 total tags in treatment: 32402250 INFO @ Thu, 08 Oct 2020 22:32:24: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:32:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:32:24: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 22:32:24: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:32:24: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 22:32:24: #1 tags after filtering in treatment: 32402100 INFO @ Thu, 08 Oct 2020 22:32:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 22:32:24: #1 finished! INFO @ Thu, 08 Oct 2020 22:32:24: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:32:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:32:26: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 22:32:26: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:32:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723703/SRX7723703.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling