Job ID = 14167557 SRX = SRX7723695 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:03 44213898 reads; of these: 44213898 (100.00%) were unpaired; of these: 4453626 (10.07%) aligned 0 times 33571474 (75.93%) aligned exactly 1 time 6188798 (14.00%) aligned >1 times 89.93% overall alignment rate Time searching: 00:15:03 Overall time: 00:15:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4871087 / 39760272 = 0.1225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:00:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:00:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:00:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:01:03: 1000000 INFO @ Fri, 10 Dec 2021 13:01:10: 2000000 INFO @ Fri, 10 Dec 2021 13:01:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:01:25: 4000000 INFO @ Fri, 10 Dec 2021 13:01:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:01:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:01:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:01:33: 1000000 INFO @ Fri, 10 Dec 2021 13:01:33: 5000000 INFO @ Fri, 10 Dec 2021 13:01:40: 2000000 INFO @ Fri, 10 Dec 2021 13:01:41: 6000000 INFO @ Fri, 10 Dec 2021 13:01:47: 3000000 INFO @ Fri, 10 Dec 2021 13:01:49: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:01:54: 4000000 INFO @ Fri, 10 Dec 2021 13:01:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:01:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:01:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:01:57: 8000000 INFO @ Fri, 10 Dec 2021 13:02:02: 5000000 INFO @ Fri, 10 Dec 2021 13:02:03: 1000000 INFO @ Fri, 10 Dec 2021 13:02:05: 9000000 INFO @ Fri, 10 Dec 2021 13:02:09: 6000000 INFO @ Fri, 10 Dec 2021 13:02:11: 2000000 INFO @ Fri, 10 Dec 2021 13:02:13: 10000000 INFO @ Fri, 10 Dec 2021 13:02:16: 7000000 INFO @ Fri, 10 Dec 2021 13:02:19: 3000000 INFO @ Fri, 10 Dec 2021 13:02:22: 11000000 INFO @ Fri, 10 Dec 2021 13:02:23: 8000000 INFO @ Fri, 10 Dec 2021 13:02:27: 4000000 INFO @ Fri, 10 Dec 2021 13:02:29: 12000000 INFO @ Fri, 10 Dec 2021 13:02:31: 9000000 INFO @ Fri, 10 Dec 2021 13:02:35: 5000000 INFO @ Fri, 10 Dec 2021 13:02:37: 13000000 INFO @ Fri, 10 Dec 2021 13:02:38: 10000000 INFO @ Fri, 10 Dec 2021 13:02:43: 6000000 INFO @ Fri, 10 Dec 2021 13:02:45: 11000000 INFO @ Fri, 10 Dec 2021 13:02:45: 14000000 INFO @ Fri, 10 Dec 2021 13:02:52: 7000000 INFO @ Fri, 10 Dec 2021 13:02:52: 12000000 INFO @ Fri, 10 Dec 2021 13:02:53: 15000000 INFO @ Fri, 10 Dec 2021 13:02:59: 8000000 INFO @ Fri, 10 Dec 2021 13:02:59: 13000000 INFO @ Fri, 10 Dec 2021 13:03:01: 16000000 INFO @ Fri, 10 Dec 2021 13:03:07: 14000000 INFO @ Fri, 10 Dec 2021 13:03:07: 9000000 INFO @ Fri, 10 Dec 2021 13:03:09: 17000000 INFO @ Fri, 10 Dec 2021 13:03:14: 15000000 INFO @ Fri, 10 Dec 2021 13:03:15: 10000000 INFO @ Fri, 10 Dec 2021 13:03:17: 18000000 INFO @ Fri, 10 Dec 2021 13:03:21: 16000000 INFO @ Fri, 10 Dec 2021 13:03:23: 11000000 INFO @ Fri, 10 Dec 2021 13:03:25: 19000000 INFO @ Fri, 10 Dec 2021 13:03:28: 17000000 INFO @ Fri, 10 Dec 2021 13:03:31: 12000000 INFO @ Fri, 10 Dec 2021 13:03:33: 20000000 INFO @ Fri, 10 Dec 2021 13:03:35: 18000000 INFO @ Fri, 10 Dec 2021 13:03:39: 13000000 INFO @ Fri, 10 Dec 2021 13:03:40: 21000000 INFO @ Fri, 10 Dec 2021 13:03:42: 19000000 INFO @ Fri, 10 Dec 2021 13:03:47: 14000000 INFO @ Fri, 10 Dec 2021 13:03:48: 22000000 INFO @ Fri, 10 Dec 2021 13:03:50: 20000000 INFO @ Fri, 10 Dec 2021 13:03:54: 15000000 INFO @ Fri, 10 Dec 2021 13:03:56: 23000000 INFO @ Fri, 10 Dec 2021 13:03:57: 21000000 INFO @ Fri, 10 Dec 2021 13:04:02: 16000000 INFO @ Fri, 10 Dec 2021 13:04:04: 22000000 INFO @ Fri, 10 Dec 2021 13:04:04: 24000000 INFO @ Fri, 10 Dec 2021 13:04:10: 17000000 INFO @ Fri, 10 Dec 2021 13:04:11: 23000000 INFO @ Fri, 10 Dec 2021 13:04:12: 25000000 INFO @ Fri, 10 Dec 2021 13:04:18: 24000000 INFO @ Fri, 10 Dec 2021 13:04:18: 18000000 INFO @ Fri, 10 Dec 2021 13:04:19: 26000000 INFO @ Fri, 10 Dec 2021 13:04:25: 25000000 INFO @ Fri, 10 Dec 2021 13:04:26: 19000000 INFO @ Fri, 10 Dec 2021 13:04:27: 27000000 INFO @ Fri, 10 Dec 2021 13:04:32: 26000000 INFO @ Fri, 10 Dec 2021 13:04:34: 20000000 INFO @ Fri, 10 Dec 2021 13:04:35: 28000000 INFO @ Fri, 10 Dec 2021 13:04:40: 27000000 INFO @ Fri, 10 Dec 2021 13:04:42: 21000000 INFO @ Fri, 10 Dec 2021 13:04:43: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:04:48: 28000000 INFO @ Fri, 10 Dec 2021 13:04:49: 22000000 INFO @ Fri, 10 Dec 2021 13:04:51: 30000000 INFO @ Fri, 10 Dec 2021 13:04:55: 29000000 INFO @ Fri, 10 Dec 2021 13:04:57: 23000000 INFO @ Fri, 10 Dec 2021 13:04:59: 31000000 INFO @ Fri, 10 Dec 2021 13:05:02: 30000000 INFO @ Fri, 10 Dec 2021 13:05:05: 24000000 INFO @ Fri, 10 Dec 2021 13:05:08: 32000000 INFO @ Fri, 10 Dec 2021 13:05:09: 31000000 INFO @ Fri, 10 Dec 2021 13:05:13: 25000000 INFO @ Fri, 10 Dec 2021 13:05:16: 33000000 INFO @ Fri, 10 Dec 2021 13:05:17: 32000000 INFO @ Fri, 10 Dec 2021 13:05:21: 26000000 INFO @ Fri, 10 Dec 2021 13:05:24: 34000000 INFO @ Fri, 10 Dec 2021 13:05:25: 33000000 INFO @ Fri, 10 Dec 2021 13:05:29: 27000000 INFO @ Fri, 10 Dec 2021 13:05:32: 34000000 INFO @ Fri, 10 Dec 2021 13:05:32: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:05:32: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:05:32: #1 total tags in treatment: 34889185 INFO @ Fri, 10 Dec 2021 13:05:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:05:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:05:33: #1 tags after filtering in treatment: 34889078 INFO @ Fri, 10 Dec 2021 13:05:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:05:33: #1 finished! INFO @ Fri, 10 Dec 2021 13:05:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:05:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:05:35: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 13:05:35: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:05:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:05:37: 28000000 INFO @ Fri, 10 Dec 2021 13:05:39: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:05:39: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:05:39: #1 total tags in treatment: 34889185 INFO @ Fri, 10 Dec 2021 13:05:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:05:40: #1 tags after filtering in treatment: 34889078 INFO @ Fri, 10 Dec 2021 13:05:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:05:40: #1 finished! INFO @ Fri, 10 Dec 2021 13:05:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:05:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:05:42: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 13:05:42: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:05:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:05:44: 29000000 INFO @ Fri, 10 Dec 2021 13:05:51: 30000000 INFO @ Fri, 10 Dec 2021 13:05:59: 31000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:06:06: 32000000 INFO @ Fri, 10 Dec 2021 13:06:13: 33000000 INFO @ Fri, 10 Dec 2021 13:06:20: 34000000 INFO @ Fri, 10 Dec 2021 13:06:27: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:06:27: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:06:27: #1 total tags in treatment: 34889185 INFO @ Fri, 10 Dec 2021 13:06:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:06:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:06:28: #1 tags after filtering in treatment: 34889078 INFO @ Fri, 10 Dec 2021 13:06:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:06:28: #1 finished! INFO @ Fri, 10 Dec 2021 13:06:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:06:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:06:30: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 13:06:30: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:06:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling