Job ID = 10166288 SRX = SRX7723695 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:54 44213898 reads; of these: 44213898 (100.00%) were unpaired; of these: 4453626 (10.07%) aligned 0 times 33571474 (75.93%) aligned exactly 1 time 6188798 (14.00%) aligned >1 times 89.93% overall alignment rate Time searching: 00:14:55 Overall time: 00:14:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4871087 / 39760272 = 0.1225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:10:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:10:50: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:10:50: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:10:55: 1000000 INFO @ Thu, 08 Oct 2020 21:11:01: 2000000 INFO @ Thu, 08 Oct 2020 21:11:07: 3000000 INFO @ Thu, 08 Oct 2020 21:11:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:11:18: 5000000 INFO @ Thu, 08 Oct 2020 21:11:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:11:20: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:11:20: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:11:25: 6000000 INFO @ Thu, 08 Oct 2020 21:11:26: 1000000 INFO @ Thu, 08 Oct 2020 21:11:31: 7000000 INFO @ Thu, 08 Oct 2020 21:11:33: 2000000 INFO @ Thu, 08 Oct 2020 21:11:37: 8000000 INFO @ Thu, 08 Oct 2020 21:11:39: 3000000 INFO @ Thu, 08 Oct 2020 21:11:44: 9000000 INFO @ Thu, 08 Oct 2020 21:11:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:11:50: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:11:50: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:11:50: 10000000 INFO @ Thu, 08 Oct 2020 21:11:53: 5000000 INFO @ Thu, 08 Oct 2020 21:11:56: 1000000 INFO @ Thu, 08 Oct 2020 21:11:56: 11000000 INFO @ Thu, 08 Oct 2020 21:11:59: 6000000 INFO @ Thu, 08 Oct 2020 21:12:03: 2000000 INFO @ Thu, 08 Oct 2020 21:12:03: 12000000 INFO @ Thu, 08 Oct 2020 21:12:06: 7000000 INFO @ Thu, 08 Oct 2020 21:12:09: 3000000 INFO @ Thu, 08 Oct 2020 21:12:09: 13000000 INFO @ Thu, 08 Oct 2020 21:12:13: 8000000 INFO @ Thu, 08 Oct 2020 21:12:15: 4000000 INFO @ Thu, 08 Oct 2020 21:12:16: 14000000 INFO @ Thu, 08 Oct 2020 21:12:20: 9000000 INFO @ Thu, 08 Oct 2020 21:12:22: 5000000 INFO @ Thu, 08 Oct 2020 21:12:22: 15000000 INFO @ Thu, 08 Oct 2020 21:12:27: 10000000 INFO @ Thu, 08 Oct 2020 21:12:28: 6000000 INFO @ Thu, 08 Oct 2020 21:12:29: 16000000 INFO @ Thu, 08 Oct 2020 21:12:33: 11000000 INFO @ Thu, 08 Oct 2020 21:12:35: 7000000 INFO @ Thu, 08 Oct 2020 21:12:35: 17000000 INFO @ Thu, 08 Oct 2020 21:12:40: 12000000 INFO @ Thu, 08 Oct 2020 21:12:41: 8000000 INFO @ Thu, 08 Oct 2020 21:12:41: 18000000 INFO @ Thu, 08 Oct 2020 21:12:46: 13000000 INFO @ Thu, 08 Oct 2020 21:12:47: 9000000 INFO @ Thu, 08 Oct 2020 21:12:48: 19000000 INFO @ Thu, 08 Oct 2020 21:12:52: 14000000 INFO @ Thu, 08 Oct 2020 21:12:54: 10000000 INFO @ Thu, 08 Oct 2020 21:12:54: 20000000 INFO @ Thu, 08 Oct 2020 21:12:59: 15000000 INFO @ Thu, 08 Oct 2020 21:13:00: 11000000 INFO @ Thu, 08 Oct 2020 21:13:00: 21000000 INFO @ Thu, 08 Oct 2020 21:13:05: 16000000 INFO @ Thu, 08 Oct 2020 21:13:06: 12000000 INFO @ Thu, 08 Oct 2020 21:13:07: 22000000 INFO @ Thu, 08 Oct 2020 21:13:11: 17000000 INFO @ Thu, 08 Oct 2020 21:13:13: 13000000 INFO @ Thu, 08 Oct 2020 21:13:13: 23000000 INFO @ Thu, 08 Oct 2020 21:13:18: 18000000 INFO @ Thu, 08 Oct 2020 21:13:19: 14000000 INFO @ Thu, 08 Oct 2020 21:13:19: 24000000 INFO @ Thu, 08 Oct 2020 21:13:24: 19000000 INFO @ Thu, 08 Oct 2020 21:13:25: 15000000 INFO @ Thu, 08 Oct 2020 21:13:26: 25000000 INFO @ Thu, 08 Oct 2020 21:13:30: 20000000 INFO @ Thu, 08 Oct 2020 21:13:32: 16000000 INFO @ Thu, 08 Oct 2020 21:13:32: 26000000 INFO @ Thu, 08 Oct 2020 21:13:37: 21000000 INFO @ Thu, 08 Oct 2020 21:13:38: 17000000 INFO @ Thu, 08 Oct 2020 21:13:39: 27000000 INFO @ Thu, 08 Oct 2020 21:13:43: 22000000 INFO @ Thu, 08 Oct 2020 21:13:44: 18000000 INFO @ Thu, 08 Oct 2020 21:13:45: 28000000 INFO @ Thu, 08 Oct 2020 21:13:49: 23000000 INFO @ Thu, 08 Oct 2020 21:13:50: 19000000 INFO @ Thu, 08 Oct 2020 21:13:52: 29000000 INFO @ Thu, 08 Oct 2020 21:13:56: 24000000 INFO @ Thu, 08 Oct 2020 21:13:57: 20000000 INFO @ Thu, 08 Oct 2020 21:13:58: 30000000 INFO @ Thu, 08 Oct 2020 21:14:02: 25000000 INFO @ Thu, 08 Oct 2020 21:14:03: 21000000 INFO @ Thu, 08 Oct 2020 21:14:04: 31000000 INFO @ Thu, 08 Oct 2020 21:14:08: 26000000 INFO @ Thu, 08 Oct 2020 21:14:09: 22000000 INFO @ Thu, 08 Oct 2020 21:14:11: 32000000 INFO @ Thu, 08 Oct 2020 21:14:15: 27000000 INFO @ Thu, 08 Oct 2020 21:14:16: 23000000 INFO @ Thu, 08 Oct 2020 21:14:17: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:14:21: 28000000 INFO @ Thu, 08 Oct 2020 21:14:22: 24000000 INFO @ Thu, 08 Oct 2020 21:14:23: 34000000 INFO @ Thu, 08 Oct 2020 21:14:28: 29000000 INFO @ Thu, 08 Oct 2020 21:14:28: 25000000 INFO @ Thu, 08 Oct 2020 21:14:29: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:14:29: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:14:29: #1 total tags in treatment: 34889185 INFO @ Thu, 08 Oct 2020 21:14:29: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:14:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:14:30: #1 tags after filtering in treatment: 34889078 INFO @ Thu, 08 Oct 2020 21:14:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:14:30: #1 finished! INFO @ Thu, 08 Oct 2020 21:14:30: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:14:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:14:32: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 21:14:32: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:14:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:14:34: 30000000 INFO @ Thu, 08 Oct 2020 21:14:35: 26000000 INFO @ Thu, 08 Oct 2020 21:14:40: 31000000 INFO @ Thu, 08 Oct 2020 21:14:41: 27000000 INFO @ Thu, 08 Oct 2020 21:14:47: 32000000 INFO @ Thu, 08 Oct 2020 21:14:47: 28000000 INFO @ Thu, 08 Oct 2020 21:14:53: 33000000 INFO @ Thu, 08 Oct 2020 21:14:54: 29000000 INFO @ Thu, 08 Oct 2020 21:14:59: 34000000 INFO @ Thu, 08 Oct 2020 21:15:00: 30000000 INFO @ Thu, 08 Oct 2020 21:15:05: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:15:05: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:15:05: #1 total tags in treatment: 34889185 INFO @ Thu, 08 Oct 2020 21:15:05: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:15:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:15:06: #1 tags after filtering in treatment: 34889078 INFO @ Thu, 08 Oct 2020 21:15:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:15:06: #1 finished! INFO @ Thu, 08 Oct 2020 21:15:06: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:15:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:15:06: 31000000 INFO @ Thu, 08 Oct 2020 21:15:08: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 21:15:08: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:15:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:15:12: 32000000 INFO @ Thu, 08 Oct 2020 21:15:18: 33000000 INFO @ Thu, 08 Oct 2020 21:15:24: 34000000 INFO @ Thu, 08 Oct 2020 21:15:29: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:15:29: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:15:29: #1 total tags in treatment: 34889185 INFO @ Thu, 08 Oct 2020 21:15:29: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:15:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:15:30: #1 tags after filtering in treatment: 34889078 INFO @ Thu, 08 Oct 2020 21:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:15:30: #1 finished! INFO @ Thu, 08 Oct 2020 21:15:30: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:15:30: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 21:15:32: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 21:15:32: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:15:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7723695/SRX7723695.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling