Job ID = 6509254 SRX = SRX764277 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:12:13 prefetch.2.10.7: 1) Downloading 'SRR1657649'... 2020-06-26T15:12:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:13:53 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:13:53 prefetch.2.10.7: 1) 'SRR1657649' was downloaded successfully 2020-06-26T15:14:45 prefetch.2.10.7: 'SRR1657649' has 10 unresolved dependencies 2020-06-26T15:14:45 prefetch.2.10.7: 2) Downloading 'ncbi-acc:AE013599.4?vdb-ctx=refseq'... 2020-06-26T15:14:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:15:01 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:15:01 prefetch.2.10.7: 2) 'ncbi-acc:AE013599.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:15:01 prefetch.2.10.7: 3) Downloading 'ncbi-acc:AE014134.5?vdb-ctx=refseq'... 2020-06-26T15:15:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:15:18 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:15:18 prefetch.2.10.7: 3) 'ncbi-acc:AE014134.5?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:15:18 prefetch.2.10.7: 4) Downloading 'ncbi-acc:AE014135.3?vdb-ctx=refseq'... 2020-06-26T15:15:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:15:30 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:15:30 prefetch.2.10.7: 4) 'ncbi-acc:AE014135.3?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:15:30 prefetch.2.10.7: 5) Downloading 'ncbi-acc:AE014296.4?vdb-ctx=refseq'... 2020-06-26T15:15:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:15:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:15:45 prefetch.2.10.7: 5) 'ncbi-acc:AE014296.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:15:45 prefetch.2.10.7: 6) Downloading 'ncbi-acc:AE014297.2?vdb-ctx=refseq'... 2020-06-26T15:15:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:16:02 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:16:02 prefetch.2.10.7: 6) 'ncbi-acc:AE014297.2?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:16:02 prefetch.2.10.7: 7) Downloading 'ncbi-acc:AE014298.4?vdb-ctx=refseq'... 2020-06-26T15:16:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:16:18 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:16:18 prefetch.2.10.7: 7) 'ncbi-acc:AE014298.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:16:18 prefetch.2.10.7: 8) Downloading 'ncbi-acc:CM000457.1?vdb-ctx=refseq'... 2020-06-26T15:16:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:16:31 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:16:31 prefetch.2.10.7: 8) 'ncbi-acc:CM000457.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:16:31 prefetch.2.10.7: 9) Downloading 'ncbi-acc:CM000458.1?vdb-ctx=refseq'... 2020-06-26T15:16:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:16:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:16:45 prefetch.2.10.7: 9) 'ncbi-acc:CM000458.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:16:45 prefetch.2.10.7: 10) Downloading 'ncbi-acc:CM000459.1?vdb-ctx=refseq'... 2020-06-26T15:16:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:16:57 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:16:57 prefetch.2.10.7: 10) 'ncbi-acc:CM000459.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T15:16:57 prefetch.2.10.7: 11) Downloading 'ncbi-acc:FA000001.1?vdb-ctx=refseq'... 2020-06-26T15:16:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:17:13 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:17:13 prefetch.2.10.7: 11) 'ncbi-acc:FA000001.1?vdb-ctx=refseq' was downloaded successfully Read 18604196 spots for SRR1657649/SRR1657649.sra Written 18604196 spots for SRR1657649/SRR1657649.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 18604196 reads; of these: 18604196 (100.00%) were unpaired; of these: 6702 (0.04%) aligned 0 times 18355345 (98.66%) aligned exactly 1 time 242149 (1.30%) aligned >1 times 99.96% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4673 / 18597494 = 0.0003 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:31:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:31:58: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:31:58: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:32:06: 1000000 INFO @ Sat, 27 Jun 2020 00:32:14: 2000000 INFO @ Sat, 27 Jun 2020 00:32:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:32:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:32:27: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:32:27: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:32:30: 4000000 INFO @ Sat, 27 Jun 2020 00:32:36: 1000000 INFO @ Sat, 27 Jun 2020 00:32:38: 5000000 INFO @ Sat, 27 Jun 2020 00:32:44: 2000000 INFO @ Sat, 27 Jun 2020 00:32:46: 6000000 INFO @ Sat, 27 Jun 2020 00:32:52: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:32:55: 7000000 INFO @ Sat, 27 Jun 2020 00:32:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:32:57: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:32:57: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:33:00: 4000000 INFO @ Sat, 27 Jun 2020 00:33:03: 8000000 INFO @ Sat, 27 Jun 2020 00:33:06: 1000000 INFO @ Sat, 27 Jun 2020 00:33:08: 5000000 INFO @ Sat, 27 Jun 2020 00:33:13: 9000000 INFO @ Sat, 27 Jun 2020 00:33:16: 2000000 INFO @ Sat, 27 Jun 2020 00:33:17: 6000000 INFO @ Sat, 27 Jun 2020 00:33:21: 10000000 INFO @ Sat, 27 Jun 2020 00:33:25: 3000000 INFO @ Sat, 27 Jun 2020 00:33:27: 7000000 INFO @ Sat, 27 Jun 2020 00:33:30: 11000000 INFO @ Sat, 27 Jun 2020 00:33:34: 4000000 INFO @ Sat, 27 Jun 2020 00:33:36: 8000000 INFO @ Sat, 27 Jun 2020 00:33:39: 12000000 INFO @ Sat, 27 Jun 2020 00:33:44: 5000000 INFO @ Sat, 27 Jun 2020 00:33:45: 9000000 INFO @ Sat, 27 Jun 2020 00:33:49: 13000000 INFO @ Sat, 27 Jun 2020 00:33:54: 6000000 INFO @ Sat, 27 Jun 2020 00:33:55: 10000000 INFO @ Sat, 27 Jun 2020 00:33:58: 14000000 INFO @ Sat, 27 Jun 2020 00:34:03: 11000000 INFO @ Sat, 27 Jun 2020 00:34:04: 7000000 INFO @ Sat, 27 Jun 2020 00:34:08: 15000000 INFO @ Sat, 27 Jun 2020 00:34:11: 12000000 INFO @ Sat, 27 Jun 2020 00:34:13: 8000000 INFO @ Sat, 27 Jun 2020 00:34:18: 16000000 INFO @ Sat, 27 Jun 2020 00:34:20: 13000000 INFO @ Sat, 27 Jun 2020 00:34:23: 9000000 INFO @ Sat, 27 Jun 2020 00:34:28: 14000000 INFO @ Sat, 27 Jun 2020 00:34:29: 17000000 INFO @ Sat, 27 Jun 2020 00:34:33: 10000000 INFO @ Sat, 27 Jun 2020 00:34:36: 15000000 INFO @ Sat, 27 Jun 2020 00:34:39: 18000000 INFO @ Sat, 27 Jun 2020 00:34:43: 11000000 INFO @ Sat, 27 Jun 2020 00:34:45: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:34:45: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:34:45: #1 total tags in treatment: 18592821 INFO @ Sat, 27 Jun 2020 00:34:45: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:34:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:34:45: 16000000 INFO @ Sat, 27 Jun 2020 00:34:45: #1 tags after filtering in treatment: 18592691 INFO @ Sat, 27 Jun 2020 00:34:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:34:45: #1 finished! INFO @ Sat, 27 Jun 2020 00:34:45: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:34:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:34:46: #2 number of paired peaks: 3 WARNING @ Sat, 27 Jun 2020 00:34:46: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:34:51: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:34:54: 17000000 INFO @ Sat, 27 Jun 2020 00:34:59: 13000000 INFO @ Sat, 27 Jun 2020 00:35:02: 18000000 INFO @ Sat, 27 Jun 2020 00:35:07: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:35:07: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:35:07: #1 total tags in treatment: 18592821 INFO @ Sat, 27 Jun 2020 00:35:07: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:35:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:35:07: #1 tags after filtering in treatment: 18592691 INFO @ Sat, 27 Jun 2020 00:35:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:35:07: #1 finished! INFO @ Sat, 27 Jun 2020 00:35:07: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:35:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:35:08: 14000000 INFO @ Sat, 27 Jun 2020 00:35:08: #2 number of paired peaks: 3 WARNING @ Sat, 27 Jun 2020 00:35:08: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:35:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:35:15: 15000000 INFO @ Sat, 27 Jun 2020 00:35:23: 16000000 INFO @ Sat, 27 Jun 2020 00:35:30: 17000000 INFO @ Sat, 27 Jun 2020 00:35:38: 18000000 INFO @ Sat, 27 Jun 2020 00:35:42: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:35:42: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:35:42: #1 total tags in treatment: 18592821 INFO @ Sat, 27 Jun 2020 00:35:42: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:35:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:35:43: #1 tags after filtering in treatment: 18592691 INFO @ Sat, 27 Jun 2020 00:35:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:35:43: #1 finished! INFO @ Sat, 27 Jun 2020 00:35:43: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:35:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:35:44: #2 number of paired peaks: 3 WARNING @ Sat, 27 Jun 2020 00:35:44: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:35:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX764277/SRX764277.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。