Job ID = 6627018 SRX = SRX7569436 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:04 17765478 reads; of these: 17765478 (100.00%) were unpaired; of these: 578289 (3.26%) aligned 0 times 14803338 (83.33%) aligned exactly 1 time 2383851 (13.42%) aligned >1 times 96.74% overall alignment rate Time searching: 00:04:04 Overall time: 00:04:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2886716 / 17187189 = 0.1680 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:34:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:34:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:34:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:34:07: 1000000 INFO @ Tue, 14 Jul 2020 09:34:13: 2000000 INFO @ Tue, 14 Jul 2020 09:34:20: 3000000 INFO @ Tue, 14 Jul 2020 09:34:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:34:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:34:30: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:34:30: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:34:34: 5000000 INFO @ Tue, 14 Jul 2020 09:34:37: 1000000 INFO @ Tue, 14 Jul 2020 09:34:41: 6000000 INFO @ Tue, 14 Jul 2020 09:34:45: 2000000 INFO @ Tue, 14 Jul 2020 09:34:49: 7000000 INFO @ Tue, 14 Jul 2020 09:34:52: 3000000 INFO @ Tue, 14 Jul 2020 09:34:56: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:34:59: 4000000 INFO @ Tue, 14 Jul 2020 09:35:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:35:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:35:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:35:03: 9000000 INFO @ Tue, 14 Jul 2020 09:35:07: 5000000 INFO @ Tue, 14 Jul 2020 09:35:07: 1000000 INFO @ Tue, 14 Jul 2020 09:35:11: 10000000 INFO @ Tue, 14 Jul 2020 09:35:14: 6000000 INFO @ Tue, 14 Jul 2020 09:35:15: 2000000 INFO @ Tue, 14 Jul 2020 09:35:18: 11000000 INFO @ Tue, 14 Jul 2020 09:35:22: 7000000 INFO @ Tue, 14 Jul 2020 09:35:22: 3000000 INFO @ Tue, 14 Jul 2020 09:35:26: 12000000 INFO @ Tue, 14 Jul 2020 09:35:29: 8000000 INFO @ Tue, 14 Jul 2020 09:35:30: 4000000 INFO @ Tue, 14 Jul 2020 09:35:34: 13000000 INFO @ Tue, 14 Jul 2020 09:35:37: 9000000 INFO @ Tue, 14 Jul 2020 09:35:37: 5000000 INFO @ Tue, 14 Jul 2020 09:35:41: 14000000 INFO @ Tue, 14 Jul 2020 09:35:44: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:35:44: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:35:44: #1 total tags in treatment: 14300473 INFO @ Tue, 14 Jul 2020 09:35:44: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:35:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:35:44: 10000000 INFO @ Tue, 14 Jul 2020 09:35:44: #1 tags after filtering in treatment: 14300446 INFO @ Tue, 14 Jul 2020 09:35:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:35:44: #1 finished! INFO @ Tue, 14 Jul 2020 09:35:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:35:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:35:44: 6000000 INFO @ Tue, 14 Jul 2020 09:35:45: #2 number of paired peaks: 52 WARNING @ Tue, 14 Jul 2020 09:35:45: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:35:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:35:51: 11000000 INFO @ Tue, 14 Jul 2020 09:35:52: 7000000 INFO @ Tue, 14 Jul 2020 09:35:59: 8000000 INFO @ Tue, 14 Jul 2020 09:35:59: 12000000 INFO @ Tue, 14 Jul 2020 09:36:06: 9000000 INFO @ Tue, 14 Jul 2020 09:36:07: 13000000 INFO @ Tue, 14 Jul 2020 09:36:14: 10000000 INFO @ Tue, 14 Jul 2020 09:36:14: 14000000 INFO @ Tue, 14 Jul 2020 09:36:17: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:36:17: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:36:17: #1 total tags in treatment: 14300473 INFO @ Tue, 14 Jul 2020 09:36:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:36:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:36:17: #1 tags after filtering in treatment: 14300446 INFO @ Tue, 14 Jul 2020 09:36:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:36:17: #1 finished! INFO @ Tue, 14 Jul 2020 09:36:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:36:17: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:36:18: #2 number of paired peaks: 52 WARNING @ Tue, 14 Jul 2020 09:36:18: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:36:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:36:21: 11000000 INFO @ Tue, 14 Jul 2020 09:36:28: 12000000 INFO @ Tue, 14 Jul 2020 09:36:35: 13000000 INFO @ Tue, 14 Jul 2020 09:36:42: 14000000 INFO @ Tue, 14 Jul 2020 09:36:44: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:36:44: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:36:44: #1 total tags in treatment: 14300473 INFO @ Tue, 14 Jul 2020 09:36:44: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:36:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:36:45: #1 tags after filtering in treatment: 14300446 INFO @ Tue, 14 Jul 2020 09:36:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:36:45: #1 finished! INFO @ Tue, 14 Jul 2020 09:36:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:36:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:36:46: #2 number of paired peaks: 52 WARNING @ Tue, 14 Jul 2020 09:36:46: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:36:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7569436/SRX7569436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。