Job ID = 14167305 SRX = SRX7541132 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 30996481 reads; of these: 30996481 (100.00%) were unpaired; of these: 12018460 (38.77%) aligned 0 times 15773137 (50.89%) aligned exactly 1 time 3204884 (10.34%) aligned >1 times 61.23% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5396654 / 18978021 = 0.2844 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:53:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:53:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:53:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:54:05: 1000000 INFO @ Fri, 10 Dec 2021 11:54:10: 2000000 INFO @ Fri, 10 Dec 2021 11:54:16: 3000000 INFO @ Fri, 10 Dec 2021 11:54:22: 4000000 INFO @ Fri, 10 Dec 2021 11:54:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:54:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:54:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:54:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:54:33: 6000000 INFO @ Fri, 10 Dec 2021 11:54:36: 1000000 INFO @ Fri, 10 Dec 2021 11:54:40: 7000000 INFO @ Fri, 10 Dec 2021 11:54:42: 2000000 INFO @ Fri, 10 Dec 2021 11:54:46: 8000000 INFO @ Fri, 10 Dec 2021 11:54:49: 3000000 INFO @ Fri, 10 Dec 2021 11:54:53: 9000000 INFO @ Fri, 10 Dec 2021 11:54:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:54:59: 10000000 INFO @ Fri, 10 Dec 2021 11:54:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:54:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:54:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:55:02: 5000000 INFO @ Fri, 10 Dec 2021 11:55:06: 11000000 INFO @ Fri, 10 Dec 2021 11:55:07: 1000000 INFO @ Fri, 10 Dec 2021 11:55:09: 6000000 INFO @ Fri, 10 Dec 2021 11:55:14: 12000000 INFO @ Fri, 10 Dec 2021 11:55:14: 2000000 INFO @ Fri, 10 Dec 2021 11:55:16: 7000000 INFO @ Fri, 10 Dec 2021 11:55:21: 13000000 INFO @ Fri, 10 Dec 2021 11:55:22: 3000000 INFO @ Fri, 10 Dec 2021 11:55:23: 8000000 INFO @ Fri, 10 Dec 2021 11:55:26: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:55:26: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:55:26: #1 total tags in treatment: 13581367 INFO @ Fri, 10 Dec 2021 11:55:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:55:26: #1 tags after filtering in treatment: 13581213 INFO @ Fri, 10 Dec 2021 11:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:55:26: #1 finished! INFO @ Fri, 10 Dec 2021 11:55:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:55:27: #2 number of paired peaks: 1289 INFO @ Fri, 10 Dec 2021 11:55:27: start model_add_line... INFO @ Fri, 10 Dec 2021 11:55:27: start X-correlation... INFO @ Fri, 10 Dec 2021 11:55:27: end of X-cor INFO @ Fri, 10 Dec 2021 11:55:27: #2 finished! INFO @ Fri, 10 Dec 2021 11:55:27: #2 predicted fragment length is 264 bps INFO @ Fri, 10 Dec 2021 11:55:27: #2 alternative fragment length(s) may be 264 bps INFO @ Fri, 10 Dec 2021 11:55:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05_model.r INFO @ Fri, 10 Dec 2021 11:55:27: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:55:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:55:30: 4000000 INFO @ Fri, 10 Dec 2021 11:55:30: 9000000 INFO @ Fri, 10 Dec 2021 11:55:37: 10000000 INFO @ Fri, 10 Dec 2021 11:55:38: 5000000 INFO @ Fri, 10 Dec 2021 11:55:44: 11000000 INFO @ Fri, 10 Dec 2021 11:55:46: 6000000 INFO @ Fri, 10 Dec 2021 11:55:52: 12000000 INFO @ Fri, 10 Dec 2021 11:55:53: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:55:57: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:55:59: 13000000 INFO @ Fri, 10 Dec 2021 11:56:01: 8000000 INFO @ Fri, 10 Dec 2021 11:56:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:56:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:56:04: #1 total tags in treatment: 13581367 INFO @ Fri, 10 Dec 2021 11:56:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:56:04: #1 tags after filtering in treatment: 13581213 INFO @ Fri, 10 Dec 2021 11:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:56:04: #1 finished! INFO @ Fri, 10 Dec 2021 11:56:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:56:05: #2 number of paired peaks: 1289 INFO @ Fri, 10 Dec 2021 11:56:05: start model_add_line... INFO @ Fri, 10 Dec 2021 11:56:05: start X-correlation... INFO @ Fri, 10 Dec 2021 11:56:05: end of X-cor INFO @ Fri, 10 Dec 2021 11:56:05: #2 finished! INFO @ Fri, 10 Dec 2021 11:56:05: #2 predicted fragment length is 264 bps INFO @ Fri, 10 Dec 2021 11:56:05: #2 alternative fragment length(s) may be 264 bps INFO @ Fri, 10 Dec 2021 11:56:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10_model.r INFO @ Fri, 10 Dec 2021 11:56:05: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:56:05: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:56:09: 9000000 INFO @ Fri, 10 Dec 2021 11:56:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:56:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:56:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.05_summits.bed INFO @ Fri, 10 Dec 2021 11:56:12: Done! pass1 - making usageList (429 chroms): 2 millis pass2 - checking and writing primary data (6721 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:56:16: 10000000 INFO @ Fri, 10 Dec 2021 11:56:23: 11000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:56:31: 12000000 INFO @ Fri, 10 Dec 2021 11:56:36: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:56:38: 13000000 INFO @ Fri, 10 Dec 2021 11:56:42: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:56:42: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:56:42: #1 total tags in treatment: 13581367 INFO @ Fri, 10 Dec 2021 11:56:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:56:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:56:43: #1 tags after filtering in treatment: 13581213 INFO @ Fri, 10 Dec 2021 11:56:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:56:43: #1 finished! INFO @ Fri, 10 Dec 2021 11:56:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:56:44: #2 number of paired peaks: 1289 INFO @ Fri, 10 Dec 2021 11:56:44: start model_add_line... INFO @ Fri, 10 Dec 2021 11:56:44: start X-correlation... INFO @ Fri, 10 Dec 2021 11:56:44: end of X-cor INFO @ Fri, 10 Dec 2021 11:56:44: #2 finished! INFO @ Fri, 10 Dec 2021 11:56:44: #2 predicted fragment length is 264 bps INFO @ Fri, 10 Dec 2021 11:56:44: #2 alternative fragment length(s) may be 264 bps INFO @ Fri, 10 Dec 2021 11:56:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20_model.r INFO @ Fri, 10 Dec 2021 11:56:44: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:56:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:56:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:56:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:56:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.10_summits.bed INFO @ Fri, 10 Dec 2021 11:56:51: Done! pass1 - making usageList (274 chroms): 2 millis pass2 - checking and writing primary data (4908 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:57:15: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:57:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:57:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:57:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541132/SRX7541132.20_summits.bed INFO @ Fri, 10 Dec 2021 11:57:31: Done! pass1 - making usageList (161 chroms): 1 millis pass2 - checking and writing primary data (3089 records, 4 fields): 9 millis CompletedMACS2peakCalling