Job ID = 14167275 SRX = SRX7541121 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:35 21367074 reads; of these: 21367074 (100.00%) were unpaired; of these: 1400004 (6.55%) aligned 0 times 17429037 (81.57%) aligned exactly 1 time 2538033 (11.88%) aligned >1 times 93.45% overall alignment rate Time searching: 00:04:36 Overall time: 00:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7994463 / 19967070 = 0.4004 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:40:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:40:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:40:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:40:25: 1000000 INFO @ Fri, 10 Dec 2021 11:40:31: 2000000 INFO @ Fri, 10 Dec 2021 11:40:36: 3000000 INFO @ Fri, 10 Dec 2021 11:40:41: 4000000 INFO @ Fri, 10 Dec 2021 11:40:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:40:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:40:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:40:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:40:52: 6000000 INFO @ Fri, 10 Dec 2021 11:40:56: 1000000 INFO @ Fri, 10 Dec 2021 11:40:58: 7000000 INFO @ Fri, 10 Dec 2021 11:41:01: 2000000 INFO @ Fri, 10 Dec 2021 11:41:04: 8000000 INFO @ Fri, 10 Dec 2021 11:41:07: 3000000 INFO @ Fri, 10 Dec 2021 11:41:09: 9000000 INFO @ Fri, 10 Dec 2021 11:41:13: 4000000 INFO @ Fri, 10 Dec 2021 11:41:16: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:41:19: 5000000 INFO @ Fri, 10 Dec 2021 11:41:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:41:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:41:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:41:22: 11000000 INFO @ Fri, 10 Dec 2021 11:41:25: 6000000 INFO @ Fri, 10 Dec 2021 11:41:27: 1000000 INFO @ Fri, 10 Dec 2021 11:41:28: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:41:28: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:41:28: #1 total tags in treatment: 11972607 INFO @ Fri, 10 Dec 2021 11:41:28: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:41:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:41:28: #1 tags after filtering in treatment: 11972530 INFO @ Fri, 10 Dec 2021 11:41:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:41:28: #1 finished! INFO @ Fri, 10 Dec 2021 11:41:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:41:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:41:30: #2 number of paired peaks: 5975 INFO @ Fri, 10 Dec 2021 11:41:30: start model_add_line... INFO @ Fri, 10 Dec 2021 11:41:30: start X-correlation... INFO @ Fri, 10 Dec 2021 11:41:30: end of X-cor INFO @ Fri, 10 Dec 2021 11:41:30: #2 finished! INFO @ Fri, 10 Dec 2021 11:41:30: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 11:41:30: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 11:41:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05_model.r INFO @ Fri, 10 Dec 2021 11:41:30: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:41:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:41:31: 7000000 INFO @ Fri, 10 Dec 2021 11:41:34: 2000000 INFO @ Fri, 10 Dec 2021 11:41:37: 8000000 INFO @ Fri, 10 Dec 2021 11:41:40: 3000000 INFO @ Fri, 10 Dec 2021 11:41:43: 9000000 INFO @ Fri, 10 Dec 2021 11:41:47: 4000000 INFO @ Fri, 10 Dec 2021 11:41:50: 10000000 INFO @ Fri, 10 Dec 2021 11:41:54: 5000000 INFO @ Fri, 10 Dec 2021 11:41:56: 11000000 INFO @ Fri, 10 Dec 2021 11:42:01: 6000000 INFO @ Fri, 10 Dec 2021 11:42:02: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:42:02: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:42:02: #1 total tags in treatment: 11972607 INFO @ Fri, 10 Dec 2021 11:42:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:42:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:42:02: #1 tags after filtering in treatment: 11972530 INFO @ Fri, 10 Dec 2021 11:42:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:42:02: #1 finished! INFO @ Fri, 10 Dec 2021 11:42:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:42:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:42:03: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:42:04: #2 number of paired peaks: 5975 INFO @ Fri, 10 Dec 2021 11:42:04: start model_add_line... INFO @ Fri, 10 Dec 2021 11:42:04: start X-correlation... INFO @ Fri, 10 Dec 2021 11:42:04: end of X-cor INFO @ Fri, 10 Dec 2021 11:42:04: #2 finished! INFO @ Fri, 10 Dec 2021 11:42:04: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 11:42:04: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 11:42:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10_model.r INFO @ Fri, 10 Dec 2021 11:42:04: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:42:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:42:07: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:42:14: 8000000 INFO @ Fri, 10 Dec 2021 11:42:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:42:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:42:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.05_summits.bed INFO @ Fri, 10 Dec 2021 11:42:18: Done! pass1 - making usageList (363 chroms): 2 millis pass2 - checking and writing primary data (8122 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:42:20: 9000000 INFO @ Fri, 10 Dec 2021 11:42:27: 10000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:42:33: 11000000 INFO @ Fri, 10 Dec 2021 11:42:37: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:42:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:42:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:42:40: #1 total tags in treatment: 11972607 INFO @ Fri, 10 Dec 2021 11:42:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:42:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:42:40: #1 tags after filtering in treatment: 11972530 INFO @ Fri, 10 Dec 2021 11:42:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:42:40: #1 finished! INFO @ Fri, 10 Dec 2021 11:42:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:42:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:42:41: #2 number of paired peaks: 5975 INFO @ Fri, 10 Dec 2021 11:42:41: start model_add_line... INFO @ Fri, 10 Dec 2021 11:42:41: start X-correlation... INFO @ Fri, 10 Dec 2021 11:42:41: end of X-cor INFO @ Fri, 10 Dec 2021 11:42:41: #2 finished! INFO @ Fri, 10 Dec 2021 11:42:41: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 11:42:41: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 11:42:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20_model.r INFO @ Fri, 10 Dec 2021 11:42:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:42:41: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:42:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:42:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:42:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.10_summits.bed INFO @ Fri, 10 Dec 2021 11:42:52: Done! pass1 - making usageList (243 chroms): 2 millis pass2 - checking and writing primary data (6579 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:43:14: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:43:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:43:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:43:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541121/SRX7541121.20_summits.bed INFO @ Fri, 10 Dec 2021 11:43:29: Done! pass1 - making usageList (166 chroms): 1 millis pass2 - checking and writing primary data (5309 records, 4 fields): 11 millis CompletedMACS2peakCalling