Job ID = 14167277 SRX = SRX7541120 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 25954152 reads; of these: 25954152 (100.00%) were unpaired; of these: 5444935 (20.98%) aligned 0 times 17586946 (67.76%) aligned exactly 1 time 2922271 (11.26%) aligned >1 times 79.02% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4614155 / 20509217 = 0.2250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:41:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:41:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:41:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:41:56: 1000000 INFO @ Fri, 10 Dec 2021 11:42:02: 2000000 INFO @ Fri, 10 Dec 2021 11:42:08: 3000000 INFO @ Fri, 10 Dec 2021 11:42:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:42:20: 5000000 INFO @ Fri, 10 Dec 2021 11:42:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:42:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:42:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:42:26: 6000000 INFO @ Fri, 10 Dec 2021 11:42:27: 1000000 INFO @ Fri, 10 Dec 2021 11:42:32: 7000000 INFO @ Fri, 10 Dec 2021 11:42:34: 2000000 INFO @ Fri, 10 Dec 2021 11:42:39: 8000000 INFO @ Fri, 10 Dec 2021 11:42:40: 3000000 INFO @ Fri, 10 Dec 2021 11:42:45: 9000000 INFO @ Fri, 10 Dec 2021 11:42:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:42:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:42:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:42:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:42:52: 10000000 INFO @ Fri, 10 Dec 2021 11:42:53: 5000000 INFO @ Fri, 10 Dec 2021 11:42:57: 1000000 INFO @ Fri, 10 Dec 2021 11:42:58: 11000000 INFO @ Fri, 10 Dec 2021 11:43:00: 6000000 INFO @ Fri, 10 Dec 2021 11:43:04: 2000000 INFO @ Fri, 10 Dec 2021 11:43:05: 12000000 INFO @ Fri, 10 Dec 2021 11:43:07: 7000000 INFO @ Fri, 10 Dec 2021 11:43:11: 3000000 INFO @ Fri, 10 Dec 2021 11:43:12: 13000000 INFO @ Fri, 10 Dec 2021 11:43:13: 8000000 INFO @ Fri, 10 Dec 2021 11:43:18: 4000000 INFO @ Fri, 10 Dec 2021 11:43:19: 14000000 INFO @ Fri, 10 Dec 2021 11:43:20: 9000000 INFO @ Fri, 10 Dec 2021 11:43:24: 5000000 INFO @ Fri, 10 Dec 2021 11:43:25: 15000000 INFO @ Fri, 10 Dec 2021 11:43:27: 10000000 INFO @ Fri, 10 Dec 2021 11:43:31: 6000000 INFO @ Fri, 10 Dec 2021 11:43:31: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:43:31: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:43:31: #1 total tags in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:43:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:43:32: #1 tags after filtering in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:43:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:43:32: #1 finished! INFO @ Fri, 10 Dec 2021 11:43:32: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:43:33: 11000000 INFO @ Fri, 10 Dec 2021 11:43:33: #2 number of paired peaks: 6820 INFO @ Fri, 10 Dec 2021 11:43:33: start model_add_line... INFO @ Fri, 10 Dec 2021 11:43:34: start X-correlation... INFO @ Fri, 10 Dec 2021 11:43:34: end of X-cor INFO @ Fri, 10 Dec 2021 11:43:34: #2 finished! INFO @ Fri, 10 Dec 2021 11:43:34: #2 predicted fragment length is 279 bps INFO @ Fri, 10 Dec 2021 11:43:34: #2 alternative fragment length(s) may be 279 bps INFO @ Fri, 10 Dec 2021 11:43:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05_model.r INFO @ Fri, 10 Dec 2021 11:43:34: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:43:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:43:38: 7000000 INFO @ Fri, 10 Dec 2021 11:43:40: 12000000 INFO @ Fri, 10 Dec 2021 11:43:44: 8000000 INFO @ Fri, 10 Dec 2021 11:43:47: 13000000 INFO @ Fri, 10 Dec 2021 11:43:51: 9000000 INFO @ Fri, 10 Dec 2021 11:43:54: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:43:57: 10000000 INFO @ Fri, 10 Dec 2021 11:44:00: 15000000 INFO @ Fri, 10 Dec 2021 11:44:04: 11000000 INFO @ Fri, 10 Dec 2021 11:44:06: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:44:06: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:44:06: #1 total tags in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:44:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:44:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:44:07: #1 tags after filtering in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:44:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:44:07: #1 finished! INFO @ Fri, 10 Dec 2021 11:44:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:44:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:44:09: #2 number of paired peaks: 6820 INFO @ Fri, 10 Dec 2021 11:44:09: start model_add_line... INFO @ Fri, 10 Dec 2021 11:44:09: start X-correlation... INFO @ Fri, 10 Dec 2021 11:44:09: end of X-cor INFO @ Fri, 10 Dec 2021 11:44:09: #2 finished! INFO @ Fri, 10 Dec 2021 11:44:09: #2 predicted fragment length is 279 bps INFO @ Fri, 10 Dec 2021 11:44:09: #2 alternative fragment length(s) may be 279 bps INFO @ Fri, 10 Dec 2021 11:44:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10_model.r INFO @ Fri, 10 Dec 2021 11:44:09: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:44:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:44:10: 12000000 INFO @ Fri, 10 Dec 2021 11:44:16: 13000000 INFO @ Fri, 10 Dec 2021 11:44:17: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:44:22: 14000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:44:28: 15000000 INFO @ Fri, 10 Dec 2021 11:44:33: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:44:33: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:44:33: #1 total tags in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:44:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:44:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:44:34: #1 tags after filtering in treatment: 15895062 INFO @ Fri, 10 Dec 2021 11:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:44:34: #1 finished! INFO @ Fri, 10 Dec 2021 11:44:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:44:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:44:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:44:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.05_summits.bed INFO @ Fri, 10 Dec 2021 11:44:35: Done! INFO @ Fri, 10 Dec 2021 11:44:36: #2 number of paired peaks: 6820 INFO @ Fri, 10 Dec 2021 11:44:36: start model_add_line... INFO @ Fri, 10 Dec 2021 11:44:36: start X-correlation... INFO @ Fri, 10 Dec 2021 11:44:36: end of X-cor INFO @ Fri, 10 Dec 2021 11:44:36: #2 finished! INFO @ Fri, 10 Dec 2021 11:44:36: #2 predicted fragment length is 279 bps INFO @ Fri, 10 Dec 2021 11:44:36: #2 alternative fragment length(s) may be 279 bps INFO @ Fri, 10 Dec 2021 11:44:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20_model.r INFO @ Fri, 10 Dec 2021 11:44:36: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:44:36: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (455 chroms): 1 millis pass2 - checking and writing primary data (7112 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:44:52: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:45:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:45:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:45:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.10_summits.bed INFO @ Fri, 10 Dec 2021 11:45:10: Done! pass1 - making usageList (415 chroms): 2 millis pass2 - checking and writing primary data (6390 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:45:18: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:45:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:45:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:45:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541120/SRX7541120.20_summits.bed INFO @ Fri, 10 Dec 2021 11:45:36: Done! pass1 - making usageList (354 chroms): 1 millis pass2 - checking and writing primary data (5547 records, 4 fields): 16 millis CompletedMACS2peakCalling