Job ID = 14167274 SRX = SRX7541118 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:19 26303999 reads; of these: 26303999 (100.00%) were unpaired; of these: 10778343 (40.98%) aligned 0 times 13868185 (52.72%) aligned exactly 1 time 1657471 (6.30%) aligned >1 times 59.02% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4266687 / 15525656 = 0.2748 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:39:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:39:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:39:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:39:37: 1000000 INFO @ Fri, 10 Dec 2021 11:39:45: 2000000 INFO @ Fri, 10 Dec 2021 11:39:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:39:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:39:58: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:39:58: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:39:58: 4000000 INFO @ Fri, 10 Dec 2021 11:40:06: 1000000 INFO @ Fri, 10 Dec 2021 11:40:06: 5000000 INFO @ Fri, 10 Dec 2021 11:40:13: 2000000 INFO @ Fri, 10 Dec 2021 11:40:13: 6000000 INFO @ Fri, 10 Dec 2021 11:40:20: 3000000 INFO @ Fri, 10 Dec 2021 11:40:20: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:40:27: 4000000 INFO @ Fri, 10 Dec 2021 11:40:27: 8000000 INFO @ Fri, 10 Dec 2021 11:40:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:40:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:40:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:40:34: 5000000 INFO @ Fri, 10 Dec 2021 11:40:34: 9000000 INFO @ Fri, 10 Dec 2021 11:40:36: 1000000 INFO @ Fri, 10 Dec 2021 11:40:42: 6000000 INFO @ Fri, 10 Dec 2021 11:40:43: 2000000 INFO @ Fri, 10 Dec 2021 11:40:43: 10000000 INFO @ Fri, 10 Dec 2021 11:40:49: 3000000 INFO @ Fri, 10 Dec 2021 11:40:49: 7000000 INFO @ Fri, 10 Dec 2021 11:40:50: 11000000 INFO @ Fri, 10 Dec 2021 11:40:52: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:40:52: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:40:52: #1 total tags in treatment: 11258969 INFO @ Fri, 10 Dec 2021 11:40:52: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:40:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:40:53: #1 tags after filtering in treatment: 11258807 INFO @ Fri, 10 Dec 2021 11:40:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:40:53: #1 finished! INFO @ Fri, 10 Dec 2021 11:40:53: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:40:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:40:55: #2 number of paired peaks: 6790 INFO @ Fri, 10 Dec 2021 11:40:55: start model_add_line... INFO @ Fri, 10 Dec 2021 11:40:55: start X-correlation... INFO @ Fri, 10 Dec 2021 11:40:55: end of X-cor INFO @ Fri, 10 Dec 2021 11:40:55: #2 finished! INFO @ Fri, 10 Dec 2021 11:40:55: #2 predicted fragment length is 241 bps INFO @ Fri, 10 Dec 2021 11:40:55: #2 alternative fragment length(s) may be 241 bps INFO @ Fri, 10 Dec 2021 11:40:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05_model.r INFO @ Fri, 10 Dec 2021 11:40:55: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:40:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:40:55: 4000000 INFO @ Fri, 10 Dec 2021 11:40:56: 8000000 INFO @ Fri, 10 Dec 2021 11:41:02: 5000000 INFO @ Fri, 10 Dec 2021 11:41:03: 9000000 INFO @ Fri, 10 Dec 2021 11:41:09: 6000000 INFO @ Fri, 10 Dec 2021 11:41:11: 10000000 INFO @ Fri, 10 Dec 2021 11:41:16: 7000000 INFO @ Fri, 10 Dec 2021 11:41:18: 11000000 INFO @ Fri, 10 Dec 2021 11:41:21: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:41:21: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:41:21: #1 total tags in treatment: 11258969 INFO @ Fri, 10 Dec 2021 11:41:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:41:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:41:22: #1 tags after filtering in treatment: 11258807 INFO @ Fri, 10 Dec 2021 11:41:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:41:22: #1 finished! INFO @ Fri, 10 Dec 2021 11:41:22: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:41:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:41:23: #2 number of paired peaks: 6790 INFO @ Fri, 10 Dec 2021 11:41:23: start model_add_line... INFO @ Fri, 10 Dec 2021 11:41:23: 8000000 INFO @ Fri, 10 Dec 2021 11:41:23: start X-correlation... INFO @ Fri, 10 Dec 2021 11:41:23: end of X-cor INFO @ Fri, 10 Dec 2021 11:41:23: #2 finished! INFO @ Fri, 10 Dec 2021 11:41:23: #2 predicted fragment length is 241 bps INFO @ Fri, 10 Dec 2021 11:41:23: #2 alternative fragment length(s) may be 241 bps INFO @ Fri, 10 Dec 2021 11:41:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10_model.r INFO @ Fri, 10 Dec 2021 11:41:23: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:41:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:41:28: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:41:30: 9000000 INFO @ Fri, 10 Dec 2021 11:41:39: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:41:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:41:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:41:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.05_summits.bed INFO @ Fri, 10 Dec 2021 11:41:45: Done! pass1 - making usageList (359 chroms): 2 millis INFO @ Fri, 10 Dec 2021 11:41:46: 11000000 pass2 - checking and writing primary data (7398 records, 4 fields): 35 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:41:49: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:41:49: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:41:49: #1 total tags in treatment: 11258969 INFO @ Fri, 10 Dec 2021 11:41:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:41:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:41:49: #1 tags after filtering in treatment: 11258807 INFO @ Fri, 10 Dec 2021 11:41:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:41:49: #1 finished! INFO @ Fri, 10 Dec 2021 11:41:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:41:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:41:51: #2 number of paired peaks: 6790 INFO @ Fri, 10 Dec 2021 11:41:51: start model_add_line... INFO @ Fri, 10 Dec 2021 11:41:51: start X-correlation... INFO @ Fri, 10 Dec 2021 11:41:51: end of X-cor INFO @ Fri, 10 Dec 2021 11:41:51: #2 finished! INFO @ Fri, 10 Dec 2021 11:41:51: #2 predicted fragment length is 241 bps INFO @ Fri, 10 Dec 2021 11:41:51: #2 alternative fragment length(s) may be 241 bps INFO @ Fri, 10 Dec 2021 11:41:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20_model.r INFO @ Fri, 10 Dec 2021 11:41:51: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:41:51: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:41:59: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:42:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:42:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:42:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.10_summits.bed INFO @ Fri, 10 Dec 2021 11:42:17: Done! pass1 - making usageList (262 chroms): 3 millis pass2 - checking and writing primary data (6579 records, 4 fields): 31 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:42:25: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:42:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:42:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:42:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7541118/SRX7541118.20_summits.bed INFO @ Fri, 10 Dec 2021 11:42:43: Done! pass1 - making usageList (105 chroms): 2 millis pass2 - checking and writing primary data (5253 records, 4 fields): 19 millis CompletedMACS2peakCalling