Job ID = 6459722 SRX = SRX751572 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:36:58 prefetch.2.10.7: 1) Downloading 'SRR1640732'... 2020-06-21T13:36:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:41:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:41:58 prefetch.2.10.7: 1) 'SRR1640732' was downloaded successfully Read 64033293 spots for SRR1640732/SRR1640732.sra Written 64033293 spots for SRR1640732/SRR1640732.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:33 64033293 reads; of these: 64033293 (100.00%) were unpaired; of these: 13985594 (21.84%) aligned 0 times 37686255 (58.85%) aligned exactly 1 time 12361444 (19.30%) aligned >1 times 78.16% overall alignment rate Time searching: 00:15:34 Overall time: 00:15:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 8009494 / 50047699 = 0.1600 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:12:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:12:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:12:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:12:22: 1000000 INFO @ Sun, 21 Jun 2020 23:12:27: 2000000 INFO @ Sun, 21 Jun 2020 23:12:31: 3000000 INFO @ Sun, 21 Jun 2020 23:12:36: 4000000 INFO @ Sun, 21 Jun 2020 23:12:40: 5000000 INFO @ Sun, 21 Jun 2020 23:12:45: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:12:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:12:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:12:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:12:49: 7000000 INFO @ Sun, 21 Jun 2020 23:12:52: 1000000 INFO @ Sun, 21 Jun 2020 23:12:54: 8000000 INFO @ Sun, 21 Jun 2020 23:12:57: 2000000 INFO @ Sun, 21 Jun 2020 23:12:58: 9000000 INFO @ Sun, 21 Jun 2020 23:13:01: 3000000 INFO @ Sun, 21 Jun 2020 23:13:03: 10000000 INFO @ Sun, 21 Jun 2020 23:13:06: 4000000 INFO @ Sun, 21 Jun 2020 23:13:07: 11000000 INFO @ Sun, 21 Jun 2020 23:13:11: 5000000 INFO @ Sun, 21 Jun 2020 23:13:12: 12000000 INFO @ Sun, 21 Jun 2020 23:13:15: 6000000 INFO @ Sun, 21 Jun 2020 23:13:16: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:13:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:13:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:13:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:13:20: 7000000 INFO @ Sun, 21 Jun 2020 23:13:21: 14000000 INFO @ Sun, 21 Jun 2020 23:13:23: 1000000 INFO @ Sun, 21 Jun 2020 23:13:24: 8000000 INFO @ Sun, 21 Jun 2020 23:13:25: 15000000 INFO @ Sun, 21 Jun 2020 23:13:28: 2000000 INFO @ Sun, 21 Jun 2020 23:13:29: 9000000 INFO @ Sun, 21 Jun 2020 23:13:30: 16000000 INFO @ Sun, 21 Jun 2020 23:13:33: 10000000 INFO @ Sun, 21 Jun 2020 23:13:33: 3000000 INFO @ Sun, 21 Jun 2020 23:13:34: 17000000 INFO @ Sun, 21 Jun 2020 23:13:38: 11000000 INFO @ Sun, 21 Jun 2020 23:13:39: 4000000 INFO @ Sun, 21 Jun 2020 23:13:39: 18000000 INFO @ Sun, 21 Jun 2020 23:13:42: 12000000 INFO @ Sun, 21 Jun 2020 23:13:43: 19000000 INFO @ Sun, 21 Jun 2020 23:13:44: 5000000 INFO @ Sun, 21 Jun 2020 23:13:47: 13000000 INFO @ Sun, 21 Jun 2020 23:13:48: 20000000 INFO @ Sun, 21 Jun 2020 23:13:49: 6000000 INFO @ Sun, 21 Jun 2020 23:13:51: 14000000 INFO @ Sun, 21 Jun 2020 23:13:52: 21000000 INFO @ Sun, 21 Jun 2020 23:13:54: 7000000 INFO @ Sun, 21 Jun 2020 23:13:56: 15000000 INFO @ Sun, 21 Jun 2020 23:13:57: 22000000 INFO @ Sun, 21 Jun 2020 23:13:59: 8000000 INFO @ Sun, 21 Jun 2020 23:14:00: 16000000 INFO @ Sun, 21 Jun 2020 23:14:01: 23000000 INFO @ Sun, 21 Jun 2020 23:14:04: 9000000 INFO @ Sun, 21 Jun 2020 23:14:05: 17000000 INFO @ Sun, 21 Jun 2020 23:14:06: 24000000 INFO @ Sun, 21 Jun 2020 23:14:09: 10000000 INFO @ Sun, 21 Jun 2020 23:14:09: 18000000 INFO @ Sun, 21 Jun 2020 23:14:10: 25000000 INFO @ Sun, 21 Jun 2020 23:14:14: 19000000 INFO @ Sun, 21 Jun 2020 23:14:15: 11000000 INFO @ Sun, 21 Jun 2020 23:14:15: 26000000 INFO @ Sun, 21 Jun 2020 23:14:18: 20000000 INFO @ Sun, 21 Jun 2020 23:14:19: 27000000 INFO @ Sun, 21 Jun 2020 23:14:20: 12000000 INFO @ Sun, 21 Jun 2020 23:14:23: 21000000 INFO @ Sun, 21 Jun 2020 23:14:24: 28000000 INFO @ Sun, 21 Jun 2020 23:14:25: 13000000 INFO @ Sun, 21 Jun 2020 23:14:28: 22000000 INFO @ Sun, 21 Jun 2020 23:14:28: 29000000 INFO @ Sun, 21 Jun 2020 23:14:30: 14000000 INFO @ Sun, 21 Jun 2020 23:14:32: 23000000 INFO @ Sun, 21 Jun 2020 23:14:33: 30000000 INFO @ Sun, 21 Jun 2020 23:14:35: 15000000 INFO @ Sun, 21 Jun 2020 23:14:37: 24000000 INFO @ Sun, 21 Jun 2020 23:14:37: 31000000 INFO @ Sun, 21 Jun 2020 23:14:40: 16000000 INFO @ Sun, 21 Jun 2020 23:14:41: 25000000 INFO @ Sun, 21 Jun 2020 23:14:42: 32000000 INFO @ Sun, 21 Jun 2020 23:14:45: 17000000 INFO @ Sun, 21 Jun 2020 23:14:46: 26000000 INFO @ Sun, 21 Jun 2020 23:14:46: 33000000 INFO @ Sun, 21 Jun 2020 23:14:50: 27000000 INFO @ Sun, 21 Jun 2020 23:14:50: 18000000 INFO @ Sun, 21 Jun 2020 23:14:51: 34000000 INFO @ Sun, 21 Jun 2020 23:14:55: 28000000 INFO @ Sun, 21 Jun 2020 23:14:55: 35000000 INFO @ Sun, 21 Jun 2020 23:14:56: 19000000 INFO @ Sun, 21 Jun 2020 23:14:59: 29000000 INFO @ Sun, 21 Jun 2020 23:15:00: 36000000 INFO @ Sun, 21 Jun 2020 23:15:01: 20000000 INFO @ Sun, 21 Jun 2020 23:15:04: 30000000 INFO @ Sun, 21 Jun 2020 23:15:05: 37000000 INFO @ Sun, 21 Jun 2020 23:15:06: 21000000 INFO @ Sun, 21 Jun 2020 23:15:08: 31000000 INFO @ Sun, 21 Jun 2020 23:15:09: 38000000 INFO @ Sun, 21 Jun 2020 23:15:11: 22000000 INFO @ Sun, 21 Jun 2020 23:15:13: 32000000 INFO @ Sun, 21 Jun 2020 23:15:14: 39000000 INFO @ Sun, 21 Jun 2020 23:15:16: 23000000 INFO @ Sun, 21 Jun 2020 23:15:17: 33000000 INFO @ Sun, 21 Jun 2020 23:15:18: 40000000 INFO @ Sun, 21 Jun 2020 23:15:21: 24000000 INFO @ Sun, 21 Jun 2020 23:15:22: 34000000 INFO @ Sun, 21 Jun 2020 23:15:23: 41000000 INFO @ Sun, 21 Jun 2020 23:15:26: 25000000 INFO @ Sun, 21 Jun 2020 23:15:27: 35000000 INFO @ Sun, 21 Jun 2020 23:15:27: 42000000 INFO @ Sun, 21 Jun 2020 23:15:28: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:15:28: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:15:28: #1 total tags in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:15:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:15:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:15:29: #1 tags after filtering in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:15:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:15:29: #1 finished! INFO @ Sun, 21 Jun 2020 23:15:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:15:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:15:31: 36000000 INFO @ Sun, 21 Jun 2020 23:15:31: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 23:15:31: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:15:31: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 23:15:32: 26000000 cut: /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:15:36: 37000000 INFO @ Sun, 21 Jun 2020 23:15:37: 27000000 INFO @ Sun, 21 Jun 2020 23:15:40: 38000000 INFO @ Sun, 21 Jun 2020 23:15:42: 28000000 INFO @ Sun, 21 Jun 2020 23:15:45: 39000000 INFO @ Sun, 21 Jun 2020 23:15:47: 29000000 INFO @ Sun, 21 Jun 2020 23:15:49: 40000000 INFO @ Sun, 21 Jun 2020 23:15:52: 30000000 INFO @ Sun, 21 Jun 2020 23:15:54: 41000000 INFO @ Sun, 21 Jun 2020 23:15:57: 31000000 INFO @ Sun, 21 Jun 2020 23:15:59: 42000000 INFO @ Sun, 21 Jun 2020 23:15:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:15:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:15:59: #1 total tags in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:15:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:15:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:16:00: #1 tags after filtering in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:16:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:16:00: #1 finished! INFO @ Sun, 21 Jun 2020 23:16:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:16:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:16:02: 32000000 INFO @ Sun, 21 Jun 2020 23:16:03: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 23:16:03: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:16:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:16:07: 33000000 INFO @ Sun, 21 Jun 2020 23:16:13: 34000000 INFO @ Sun, 21 Jun 2020 23:16:18: 35000000 INFO @ Sun, 21 Jun 2020 23:16:23: 36000000 INFO @ Sun, 21 Jun 2020 23:16:29: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:16:34: 38000000 INFO @ Sun, 21 Jun 2020 23:16:39: 39000000 INFO @ Sun, 21 Jun 2020 23:16:44: 40000000 INFO @ Sun, 21 Jun 2020 23:16:50: 41000000 INFO @ Sun, 21 Jun 2020 23:16:55: 42000000 INFO @ Sun, 21 Jun 2020 23:16:56: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:16:56: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:16:56: #1 total tags in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:16:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:16:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:16:57: #1 tags after filtering in treatment: 42038205 INFO @ Sun, 21 Jun 2020 23:16:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:16:57: #1 finished! INFO @ Sun, 21 Jun 2020 23:16:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:16:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:16:59: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 23:16:59: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:16:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751572/SRX751572.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。