Job ID = 6459719 SRX = SRX751571 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:20:01 prefetch.2.10.7: 1) Downloading 'SRR1640731'... 2020-06-21T13:20:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:27:18 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:27:18 prefetch.2.10.7: 1) 'SRR1640731' was downloaded successfully Read 58628307 spots for SRR1640731/SRR1640731.sra Written 58628307 spots for SRR1640731/SRR1640731.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:18 58628307 reads; of these: 58628307 (100.00%) were unpaired; of these: 4881941 (8.33%) aligned 0 times 40730689 (69.47%) aligned exactly 1 time 13015677 (22.20%) aligned >1 times 91.67% overall alignment rate Time searching: 00:19:18 Overall time: 00:19:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9822975 / 53746366 = 0.1828 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:03:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:03:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:03:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:03:53: 1000000 INFO @ Sun, 21 Jun 2020 23:03:59: 2000000 INFO @ Sun, 21 Jun 2020 23:04:05: 3000000 INFO @ Sun, 21 Jun 2020 23:04:10: 4000000 INFO @ Sun, 21 Jun 2020 23:04:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:04:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:04:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:04:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:04:22: 6000000 INFO @ Sun, 21 Jun 2020 23:04:25: 1000000 INFO @ Sun, 21 Jun 2020 23:04:28: 7000000 INFO @ Sun, 21 Jun 2020 23:04:31: 2000000 INFO @ Sun, 21 Jun 2020 23:04:35: 8000000 INFO @ Sun, 21 Jun 2020 23:04:38: 3000000 INFO @ Sun, 21 Jun 2020 23:04:41: 9000000 INFO @ Sun, 21 Jun 2020 23:04:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:04:48: 10000000 INFO @ Sun, 21 Jun 2020 23:04:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:04:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:04:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:04:52: 5000000 INFO @ Sun, 21 Jun 2020 23:04:55: 11000000 INFO @ Sun, 21 Jun 2020 23:04:55: 1000000 INFO @ Sun, 21 Jun 2020 23:04:59: 6000000 INFO @ Sun, 21 Jun 2020 23:05:02: 12000000 INFO @ Sun, 21 Jun 2020 23:05:03: 2000000 INFO @ Sun, 21 Jun 2020 23:05:06: 7000000 INFO @ Sun, 21 Jun 2020 23:05:09: 13000000 INFO @ Sun, 21 Jun 2020 23:05:10: 3000000 INFO @ Sun, 21 Jun 2020 23:05:13: 8000000 INFO @ Sun, 21 Jun 2020 23:05:16: 14000000 INFO @ Sun, 21 Jun 2020 23:05:17: 4000000 INFO @ Sun, 21 Jun 2020 23:05:20: 9000000 INFO @ Sun, 21 Jun 2020 23:05:23: 15000000 INFO @ Sun, 21 Jun 2020 23:05:25: 5000000 INFO @ Sun, 21 Jun 2020 23:05:28: 10000000 INFO @ Sun, 21 Jun 2020 23:05:30: 16000000 INFO @ Sun, 21 Jun 2020 23:05:32: 6000000 INFO @ Sun, 21 Jun 2020 23:05:35: 11000000 INFO @ Sun, 21 Jun 2020 23:05:37: 17000000 INFO @ Sun, 21 Jun 2020 23:05:39: 7000000 INFO @ Sun, 21 Jun 2020 23:05:42: 12000000 INFO @ Sun, 21 Jun 2020 23:05:45: 18000000 INFO @ Sun, 21 Jun 2020 23:05:47: 8000000 INFO @ Sun, 21 Jun 2020 23:05:50: 13000000 INFO @ Sun, 21 Jun 2020 23:05:52: 19000000 INFO @ Sun, 21 Jun 2020 23:05:54: 9000000 INFO @ Sun, 21 Jun 2020 23:05:57: 14000000 INFO @ Sun, 21 Jun 2020 23:05:59: 20000000 INFO @ Sun, 21 Jun 2020 23:06:02: 10000000 INFO @ Sun, 21 Jun 2020 23:06:04: 15000000 INFO @ Sun, 21 Jun 2020 23:06:06: 21000000 INFO @ Sun, 21 Jun 2020 23:06:09: 11000000 INFO @ Sun, 21 Jun 2020 23:06:11: 16000000 INFO @ Sun, 21 Jun 2020 23:06:13: 22000000 INFO @ Sun, 21 Jun 2020 23:06:16: 12000000 INFO @ Sun, 21 Jun 2020 23:06:18: 17000000 INFO @ Sun, 21 Jun 2020 23:06:20: 23000000 INFO @ Sun, 21 Jun 2020 23:06:23: 13000000 INFO @ Sun, 21 Jun 2020 23:06:25: 18000000 INFO @ Sun, 21 Jun 2020 23:06:27: 24000000 INFO @ Sun, 21 Jun 2020 23:06:29: 14000000 INFO @ Sun, 21 Jun 2020 23:06:32: 19000000 INFO @ Sun, 21 Jun 2020 23:06:34: 25000000 INFO @ Sun, 21 Jun 2020 23:06:36: 15000000 INFO @ Sun, 21 Jun 2020 23:06:38: 20000000 INFO @ Sun, 21 Jun 2020 23:06:40: 26000000 INFO @ Sun, 21 Jun 2020 23:06:43: 16000000 INFO @ Sun, 21 Jun 2020 23:06:45: 21000000 INFO @ Sun, 21 Jun 2020 23:06:47: 27000000 INFO @ Sun, 21 Jun 2020 23:06:50: 17000000 INFO @ Sun, 21 Jun 2020 23:06:52: 22000000 INFO @ Sun, 21 Jun 2020 23:06:54: 28000000 INFO @ Sun, 21 Jun 2020 23:06:57: 18000000 INFO @ Sun, 21 Jun 2020 23:06:59: 23000000 INFO @ Sun, 21 Jun 2020 23:07:01: 29000000 INFO @ Sun, 21 Jun 2020 23:07:04: 19000000 INFO @ Sun, 21 Jun 2020 23:07:06: 24000000 INFO @ Sun, 21 Jun 2020 23:07:08: 30000000 INFO @ Sun, 21 Jun 2020 23:07:11: 20000000 INFO @ Sun, 21 Jun 2020 23:07:13: 25000000 INFO @ Sun, 21 Jun 2020 23:07:15: 31000000 INFO @ Sun, 21 Jun 2020 23:07:18: 21000000 INFO @ Sun, 21 Jun 2020 23:07:20: 26000000 INFO @ Sun, 21 Jun 2020 23:07:21: 32000000 INFO @ Sun, 21 Jun 2020 23:07:25: 22000000 INFO @ Sun, 21 Jun 2020 23:07:27: 27000000 INFO @ Sun, 21 Jun 2020 23:07:28: 33000000 INFO @ Sun, 21 Jun 2020 23:07:32: 23000000 INFO @ Sun, 21 Jun 2020 23:07:34: 28000000 INFO @ Sun, 21 Jun 2020 23:07:35: 34000000 INFO @ Sun, 21 Jun 2020 23:07:39: 24000000 INFO @ Sun, 21 Jun 2020 23:07:40: 29000000 INFO @ Sun, 21 Jun 2020 23:07:42: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:07:46: 25000000 INFO @ Sun, 21 Jun 2020 23:07:47: 30000000 INFO @ Sun, 21 Jun 2020 23:07:49: 36000000 INFO @ Sun, 21 Jun 2020 23:07:52: 26000000 INFO @ Sun, 21 Jun 2020 23:07:54: 31000000 INFO @ Sun, 21 Jun 2020 23:07:56: 37000000 INFO @ Sun, 21 Jun 2020 23:07:59: 27000000 INFO @ Sun, 21 Jun 2020 23:08:01: 32000000 INFO @ Sun, 21 Jun 2020 23:08:02: 38000000 INFO @ Sun, 21 Jun 2020 23:08:06: 28000000 INFO @ Sun, 21 Jun 2020 23:08:07: 33000000 INFO @ Sun, 21 Jun 2020 23:08:09: 39000000 INFO @ Sun, 21 Jun 2020 23:08:12: 29000000 INFO @ Sun, 21 Jun 2020 23:08:14: 34000000 INFO @ Sun, 21 Jun 2020 23:08:16: 40000000 INFO @ Sun, 21 Jun 2020 23:08:19: 30000000 INFO @ Sun, 21 Jun 2020 23:08:21: 35000000 INFO @ Sun, 21 Jun 2020 23:08:23: 41000000 INFO @ Sun, 21 Jun 2020 23:08:25: 31000000 INFO @ Sun, 21 Jun 2020 23:08:28: 36000000 INFO @ Sun, 21 Jun 2020 23:08:29: 42000000 INFO @ Sun, 21 Jun 2020 23:08:32: 32000000 INFO @ Sun, 21 Jun 2020 23:08:34: 37000000 INFO @ Sun, 21 Jun 2020 23:08:36: 43000000 INFO @ Sun, 21 Jun 2020 23:08:39: 33000000 INFO @ Sun, 21 Jun 2020 23:08:41: 38000000 INFO @ Sun, 21 Jun 2020 23:08:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:08:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:08:43: #1 total tags in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:08:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:08:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:08:44: #1 tags after filtering in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:08:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:08:44: #1 finished! INFO @ Sun, 21 Jun 2020 23:08:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:08:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:08:45: 34000000 INFO @ Sun, 21 Jun 2020 23:08:46: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 23:08:46: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:08:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:08:48: 39000000 INFO @ Sun, 21 Jun 2020 23:08:52: 35000000 INFO @ Sun, 21 Jun 2020 23:08:54: 40000000 INFO @ Sun, 21 Jun 2020 23:08:59: 36000000 INFO @ Sun, 21 Jun 2020 23:09:01: 41000000 INFO @ Sun, 21 Jun 2020 23:09:05: 37000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:09:07: 42000000 INFO @ Sun, 21 Jun 2020 23:09:12: 38000000 INFO @ Sun, 21 Jun 2020 23:09:14: 43000000 INFO @ Sun, 21 Jun 2020 23:09:18: 39000000 INFO @ Sun, 21 Jun 2020 23:09:21: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:09:21: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:09:21: #1 total tags in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:09:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:09:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:09:22: #1 tags after filtering in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:09:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:09:22: #1 finished! INFO @ Sun, 21 Jun 2020 23:09:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:09:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:09:24: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 23:09:24: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:09:24: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 23:09:25: 40000000 cut: /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:09:31: 41000000 INFO @ Sun, 21 Jun 2020 23:09:37: 42000000 INFO @ Sun, 21 Jun 2020 23:09:43: 43000000 INFO @ Sun, 21 Jun 2020 23:09:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:09:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:09:49: #1 total tags in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:09:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:09:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:09:50: #1 tags after filtering in treatment: 43923391 INFO @ Sun, 21 Jun 2020 23:09:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:09:50: #1 finished! INFO @ Sun, 21 Jun 2020 23:09:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:09:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:09:53: #2 number of paired peaks: 67 WARNING @ Sun, 21 Jun 2020 23:09:53: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:09:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX751571/SRX751571.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling