Job ID = 6530076 SRX = SRX751570 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:44 44147907 reads; of these: 44147907 (100.00%) were unpaired; of these: 1741719 (3.95%) aligned 0 times 15475655 (35.05%) aligned exactly 1 time 26930533 (61.00%) aligned >1 times 96.05% overall alignment rate Time searching: 00:18:44 Overall time: 00:18:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13767933 / 42406188 = 0.3247 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:33:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:33:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:33:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:33:29: 1000000 INFO @ Tue, 30 Jun 2020 03:33:35: 2000000 INFO @ Tue, 30 Jun 2020 03:33:41: 3000000 INFO @ Tue, 30 Jun 2020 03:33:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:33:53: 5000000 INFO @ Tue, 30 Jun 2020 03:33:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:33:53: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:33:53: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:33:59: 6000000 INFO @ Tue, 30 Jun 2020 03:34:00: 1000000 INFO @ Tue, 30 Jun 2020 03:34:05: 7000000 INFO @ Tue, 30 Jun 2020 03:34:06: 2000000 INFO @ Tue, 30 Jun 2020 03:34:11: 8000000 INFO @ Tue, 30 Jun 2020 03:34:12: 3000000 INFO @ Tue, 30 Jun 2020 03:34:17: 9000000 INFO @ Tue, 30 Jun 2020 03:34:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:34:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:34:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:34:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:34:24: 10000000 INFO @ Tue, 30 Jun 2020 03:34:24: 5000000 INFO @ Tue, 30 Jun 2020 03:34:30: 6000000 INFO @ Tue, 30 Jun 2020 03:34:31: 11000000 INFO @ Tue, 30 Jun 2020 03:34:31: 1000000 INFO @ Tue, 30 Jun 2020 03:34:36: 7000000 INFO @ Tue, 30 Jun 2020 03:34:38: 12000000 INFO @ Tue, 30 Jun 2020 03:34:38: 2000000 INFO @ Tue, 30 Jun 2020 03:34:43: 8000000 INFO @ Tue, 30 Jun 2020 03:34:44: 3000000 INFO @ Tue, 30 Jun 2020 03:34:45: 13000000 INFO @ Tue, 30 Jun 2020 03:34:49: 9000000 INFO @ Tue, 30 Jun 2020 03:34:50: 4000000 INFO @ Tue, 30 Jun 2020 03:34:52: 14000000 INFO @ Tue, 30 Jun 2020 03:34:56: 10000000 INFO @ Tue, 30 Jun 2020 03:34:57: 5000000 INFO @ Tue, 30 Jun 2020 03:34:59: 15000000 INFO @ Tue, 30 Jun 2020 03:35:02: 11000000 INFO @ Tue, 30 Jun 2020 03:35:04: 6000000 INFO @ Tue, 30 Jun 2020 03:35:05: 16000000 INFO @ Tue, 30 Jun 2020 03:35:08: 12000000 INFO @ Tue, 30 Jun 2020 03:35:11: 7000000 INFO @ Tue, 30 Jun 2020 03:35:12: 17000000 INFO @ Tue, 30 Jun 2020 03:35:14: 13000000 INFO @ Tue, 30 Jun 2020 03:35:17: 8000000 INFO @ Tue, 30 Jun 2020 03:35:18: 18000000 INFO @ Tue, 30 Jun 2020 03:35:20: 14000000 INFO @ Tue, 30 Jun 2020 03:35:24: 9000000 INFO @ Tue, 30 Jun 2020 03:35:25: 19000000 INFO @ Tue, 30 Jun 2020 03:35:25: 15000000 INFO @ Tue, 30 Jun 2020 03:35:31: 10000000 INFO @ Tue, 30 Jun 2020 03:35:31: 16000000 INFO @ Tue, 30 Jun 2020 03:35:32: 20000000 INFO @ Tue, 30 Jun 2020 03:35:37: 17000000 INFO @ Tue, 30 Jun 2020 03:35:38: 11000000 INFO @ Tue, 30 Jun 2020 03:35:39: 21000000 INFO @ Tue, 30 Jun 2020 03:35:43: 18000000 INFO @ Tue, 30 Jun 2020 03:35:45: 12000000 INFO @ Tue, 30 Jun 2020 03:35:47: 22000000 INFO @ Tue, 30 Jun 2020 03:35:49: 19000000 INFO @ Tue, 30 Jun 2020 03:35:52: 13000000 INFO @ Tue, 30 Jun 2020 03:35:54: 23000000 INFO @ Tue, 30 Jun 2020 03:35:56: 20000000 INFO @ Tue, 30 Jun 2020 03:35:59: 14000000 INFO @ Tue, 30 Jun 2020 03:36:02: 24000000 INFO @ Tue, 30 Jun 2020 03:36:03: 21000000 INFO @ Tue, 30 Jun 2020 03:36:06: 15000000 INFO @ Tue, 30 Jun 2020 03:36:09: 22000000 INFO @ Tue, 30 Jun 2020 03:36:09: 25000000 INFO @ Tue, 30 Jun 2020 03:36:14: 16000000 INFO @ Tue, 30 Jun 2020 03:36:16: 23000000 INFO @ Tue, 30 Jun 2020 03:36:17: 26000000 INFO @ Tue, 30 Jun 2020 03:36:21: 17000000 INFO @ Tue, 30 Jun 2020 03:36:22: 24000000 INFO @ Tue, 30 Jun 2020 03:36:25: 27000000 INFO @ Tue, 30 Jun 2020 03:36:29: 25000000 INFO @ Tue, 30 Jun 2020 03:36:29: 18000000 INFO @ Tue, 30 Jun 2020 03:36:33: 28000000 INFO @ Tue, 30 Jun 2020 03:36:36: 26000000 INFO @ Tue, 30 Jun 2020 03:36:37: 19000000 INFO @ Tue, 30 Jun 2020 03:36:38: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:36:38: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:36:38: #1 total tags in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:36:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:36:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:36:39: #1 tags after filtering in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:36:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:36:39: #1 finished! INFO @ Tue, 30 Jun 2020 03:36:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:36:39: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 03:36:41: #2 number of paired peaks: 1550 INFO @ Tue, 30 Jun 2020 03:36:41: start model_add_line... INFO @ Tue, 30 Jun 2020 03:36:41: start X-correlation... INFO @ Tue, 30 Jun 2020 03:36:41: end of X-cor INFO @ Tue, 30 Jun 2020 03:36:41: #2 finished! INFO @ Tue, 30 Jun 2020 03:36:41: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:36:41: #2 alternative fragment length(s) may be 1,33 bps INFO @ Tue, 30 Jun 2020 03:36:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05_model.r WARNING @ Tue, 30 Jun 2020 03:36:41: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:36:41: #2 You may need to consider one of the other alternative d(s): 1,33 WARNING @ Tue, 30 Jun 2020 03:36:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:36:41: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:36:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:36:44: 27000000 INFO @ Tue, 30 Jun 2020 03:36:45: 20000000 INFO @ Tue, 30 Jun 2020 03:36:51: 28000000 INFO @ Tue, 30 Jun 2020 03:36:53: 21000000 INFO @ Tue, 30 Jun 2020 03:36:56: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:36:56: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:36:56: #1 total tags in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:36:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:36:57: #1 tags after filtering in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:36:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:36:57: #1 finished! INFO @ Tue, 30 Jun 2020 03:36:57: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:36:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:36:59: #2 number of paired peaks: 1550 INFO @ Tue, 30 Jun 2020 03:36:59: start model_add_line... INFO @ Tue, 30 Jun 2020 03:36:59: start X-correlation... INFO @ Tue, 30 Jun 2020 03:36:59: end of X-cor INFO @ Tue, 30 Jun 2020 03:36:59: #2 finished! INFO @ Tue, 30 Jun 2020 03:36:59: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:36:59: #2 alternative fragment length(s) may be 1,33 bps INFO @ Tue, 30 Jun 2020 03:36:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10_model.r WARNING @ Tue, 30 Jun 2020 03:36:59: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:36:59: #2 You may need to consider one of the other alternative d(s): 1,33 WARNING @ Tue, 30 Jun 2020 03:36:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:36:59: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:36:59: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:37:01: 22000000 INFO @ Tue, 30 Jun 2020 03:37:09: 23000000 INFO @ Tue, 30 Jun 2020 03:37:16: 24000000 INFO @ Tue, 30 Jun 2020 03:37:17: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:37:25: 25000000 INFO @ Tue, 30 Jun 2020 03:37:32: 26000000 INFO @ Tue, 30 Jun 2020 03:37:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05_peaks.xls INFO @ Tue, 30 Jun 2020 03:37:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:37:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.05_summits.bed INFO @ Tue, 30 Jun 2020 03:37:34: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:37:35: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:37:40: 27000000 INFO @ Tue, 30 Jun 2020 03:37:49: 28000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 03:37:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10_peaks.xls INFO @ Tue, 30 Jun 2020 03:37:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:37:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.10_summits.bed INFO @ Tue, 30 Jun 2020 03:37:52: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:37:54: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:37:54: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:37:54: #1 total tags in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:37:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:37:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:37:55: #1 tags after filtering in treatment: 28638255 INFO @ Tue, 30 Jun 2020 03:37:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:37:55: #1 finished! INFO @ Tue, 30 Jun 2020 03:37:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:37:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:37:57: #2 number of paired peaks: 1550 INFO @ Tue, 30 Jun 2020 03:37:57: start model_add_line... INFO @ Tue, 30 Jun 2020 03:37:57: start X-correlation... INFO @ Tue, 30 Jun 2020 03:37:57: end of X-cor INFO @ Tue, 30 Jun 2020 03:37:57: #2 finished! INFO @ Tue, 30 Jun 2020 03:37:57: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:37:57: #2 alternative fragment length(s) may be 1,33 bps INFO @ Tue, 30 Jun 2020 03:37:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20_model.r WARNING @ Tue, 30 Jun 2020 03:37:57: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:37:57: #2 You may need to consider one of the other alternative d(s): 1,33 WARNING @ Tue, 30 Jun 2020 03:37:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:37:57: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:37:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:38:33: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:38:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20_peaks.xls INFO @ Tue, 30 Jun 2020 03:38:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:38:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751570/SRX751570.20_summits.bed INFO @ Tue, 30 Jun 2020 03:38:50: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling