Job ID = 6530074 SRX = SRX751567 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:28 42375548 reads; of these: 42375548 (100.00%) were unpaired; of these: 1489652 (3.52%) aligned 0 times 13256668 (31.28%) aligned exactly 1 time 27629228 (65.20%) aligned >1 times 96.48% overall alignment rate Time searching: 00:17:28 Overall time: 00:17:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14698425 / 40885896 = 0.3595 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:39:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:39:17: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:39:17: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:39:23: 1000000 INFO @ Tue, 30 Jun 2020 03:39:29: 2000000 INFO @ Tue, 30 Jun 2020 03:39:34: 3000000 INFO @ Tue, 30 Jun 2020 03:39:40: 4000000 INFO @ Tue, 30 Jun 2020 03:39:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:39:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:39:47: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:39:47: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:39:51: 6000000 INFO @ Tue, 30 Jun 2020 03:39:54: 1000000 INFO @ Tue, 30 Jun 2020 03:39:58: 7000000 INFO @ Tue, 30 Jun 2020 03:40:01: 2000000 INFO @ Tue, 30 Jun 2020 03:40:05: 8000000 INFO @ Tue, 30 Jun 2020 03:40:07: 3000000 INFO @ Tue, 30 Jun 2020 03:40:11: 9000000 INFO @ Tue, 30 Jun 2020 03:40:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:40:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:40:17: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:40:17: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:40:18: 10000000 INFO @ Tue, 30 Jun 2020 03:40:21: 5000000 INFO @ Tue, 30 Jun 2020 03:40:24: 1000000 INFO @ Tue, 30 Jun 2020 03:40:24: 11000000 INFO @ Tue, 30 Jun 2020 03:40:27: 6000000 INFO @ Tue, 30 Jun 2020 03:40:31: 2000000 INFO @ Tue, 30 Jun 2020 03:40:31: 12000000 INFO @ Tue, 30 Jun 2020 03:40:34: 7000000 INFO @ Tue, 30 Jun 2020 03:40:37: 3000000 INFO @ Tue, 30 Jun 2020 03:40:38: 13000000 INFO @ Tue, 30 Jun 2020 03:40:41: 8000000 INFO @ Tue, 30 Jun 2020 03:40:44: 4000000 INFO @ Tue, 30 Jun 2020 03:40:44: 14000000 INFO @ Tue, 30 Jun 2020 03:40:47: 9000000 INFO @ Tue, 30 Jun 2020 03:40:50: 5000000 INFO @ Tue, 30 Jun 2020 03:40:51: 15000000 INFO @ Tue, 30 Jun 2020 03:40:54: 10000000 INFO @ Tue, 30 Jun 2020 03:40:57: 6000000 INFO @ Tue, 30 Jun 2020 03:40:57: 16000000 INFO @ Tue, 30 Jun 2020 03:41:01: 11000000 INFO @ Tue, 30 Jun 2020 03:41:04: 7000000 INFO @ Tue, 30 Jun 2020 03:41:04: 17000000 INFO @ Tue, 30 Jun 2020 03:41:08: 12000000 INFO @ Tue, 30 Jun 2020 03:41:10: 8000000 INFO @ Tue, 30 Jun 2020 03:41:11: 18000000 INFO @ Tue, 30 Jun 2020 03:41:14: 13000000 INFO @ Tue, 30 Jun 2020 03:41:17: 9000000 INFO @ Tue, 30 Jun 2020 03:41:17: 19000000 INFO @ Tue, 30 Jun 2020 03:41:21: 14000000 INFO @ Tue, 30 Jun 2020 03:41:23: 10000000 INFO @ Tue, 30 Jun 2020 03:41:24: 20000000 INFO @ Tue, 30 Jun 2020 03:41:27: 15000000 INFO @ Tue, 30 Jun 2020 03:41:30: 11000000 INFO @ Tue, 30 Jun 2020 03:41:31: 21000000 INFO @ Tue, 30 Jun 2020 03:41:34: 16000000 INFO @ Tue, 30 Jun 2020 03:41:36: 12000000 INFO @ Tue, 30 Jun 2020 03:41:37: 22000000 INFO @ Tue, 30 Jun 2020 03:41:41: 17000000 INFO @ Tue, 30 Jun 2020 03:41:43: 13000000 INFO @ Tue, 30 Jun 2020 03:41:44: 23000000 INFO @ Tue, 30 Jun 2020 03:41:47: 18000000 INFO @ Tue, 30 Jun 2020 03:41:49: 14000000 INFO @ Tue, 30 Jun 2020 03:41:51: 24000000 INFO @ Tue, 30 Jun 2020 03:41:54: 19000000 INFO @ Tue, 30 Jun 2020 03:41:56: 15000000 INFO @ Tue, 30 Jun 2020 03:41:57: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 03:42:01: 20000000 INFO @ Tue, 30 Jun 2020 03:42:02: 16000000 INFO @ Tue, 30 Jun 2020 03:42:04: 26000000 INFO @ Tue, 30 Jun 2020 03:42:05: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:42:05: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:42:05: #1 total tags in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:42:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:42:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:42:06: #1 tags after filtering in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:42:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:42:06: #1 finished! INFO @ Tue, 30 Jun 2020 03:42:06: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:42:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:42:08: 21000000 INFO @ Tue, 30 Jun 2020 03:42:08: #2 number of paired peaks: 2377 INFO @ Tue, 30 Jun 2020 03:42:08: start model_add_line... INFO @ Tue, 30 Jun 2020 03:42:08: start X-correlation... INFO @ Tue, 30 Jun 2020 03:42:08: end of X-cor INFO @ Tue, 30 Jun 2020 03:42:08: #2 finished! INFO @ Tue, 30 Jun 2020 03:42:08: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:42:08: #2 alternative fragment length(s) may be 1,39,598 bps INFO @ Tue, 30 Jun 2020 03:42:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05_model.r WARNING @ Tue, 30 Jun 2020 03:42:08: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:42:08: #2 You may need to consider one of the other alternative d(s): 1,39,598 WARNING @ Tue, 30 Jun 2020 03:42:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:42:08: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:42:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:42:09: 17000000 INFO @ Tue, 30 Jun 2020 03:42:14: 22000000 INFO @ Tue, 30 Jun 2020 03:42:15: 18000000 INFO @ Tue, 30 Jun 2020 03:42:21: 23000000 INFO @ Tue, 30 Jun 2020 03:42:22: 19000000 INFO @ Tue, 30 Jun 2020 03:42:28: 24000000 INFO @ Tue, 30 Jun 2020 03:42:29: 20000000 INFO @ Tue, 30 Jun 2020 03:42:34: 25000000 INFO @ Tue, 30 Jun 2020 03:42:35: 21000000 INFO @ Tue, 30 Jun 2020 03:42:41: 26000000 INFO @ Tue, 30 Jun 2020 03:42:42: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:42:42: 22000000 INFO @ Tue, 30 Jun 2020 03:42:42: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:42:42: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:42:42: #1 total tags in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:42:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:42:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:42:43: #1 tags after filtering in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:42:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:42:43: #1 finished! INFO @ Tue, 30 Jun 2020 03:42:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:42:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:42:45: #2 number of paired peaks: 2377 INFO @ Tue, 30 Jun 2020 03:42:45: start model_add_line... INFO @ Tue, 30 Jun 2020 03:42:45: start X-correlation... INFO @ Tue, 30 Jun 2020 03:42:45: end of X-cor INFO @ Tue, 30 Jun 2020 03:42:45: #2 finished! INFO @ Tue, 30 Jun 2020 03:42:45: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:42:45: #2 alternative fragment length(s) may be 1,39,598 bps INFO @ Tue, 30 Jun 2020 03:42:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10_model.r WARNING @ Tue, 30 Jun 2020 03:42:45: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:42:45: #2 You may need to consider one of the other alternative d(s): 1,39,598 WARNING @ Tue, 30 Jun 2020 03:42:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:42:45: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:42:45: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 03:42:48: 23000000 INFO @ Tue, 30 Jun 2020 03:42:54: 24000000 INFO @ Tue, 30 Jun 2020 03:42:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05_peaks.xls INFO @ Tue, 30 Jun 2020 03:42:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:42:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.05_summits.bed INFO @ Tue, 30 Jun 2020 03:42:58: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:43:00: 25000000 INFO @ Tue, 30 Jun 2020 03:43:07: 26000000 INFO @ Tue, 30 Jun 2020 03:43:08: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:43:08: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:43:08: #1 total tags in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:43:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:43:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:43:08: #1 tags after filtering in treatment: 26187471 INFO @ Tue, 30 Jun 2020 03:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:43:08: #1 finished! INFO @ Tue, 30 Jun 2020 03:43:08: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:43:10: #2 number of paired peaks: 2377 INFO @ Tue, 30 Jun 2020 03:43:10: start model_add_line... INFO @ Tue, 30 Jun 2020 03:43:11: start X-correlation... INFO @ Tue, 30 Jun 2020 03:43:11: end of X-cor INFO @ Tue, 30 Jun 2020 03:43:11: #2 finished! INFO @ Tue, 30 Jun 2020 03:43:11: #2 predicted fragment length is 1 bps INFO @ Tue, 30 Jun 2020 03:43:11: #2 alternative fragment length(s) may be 1,39,598 bps INFO @ Tue, 30 Jun 2020 03:43:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20_model.r WARNING @ Tue, 30 Jun 2020 03:43:11: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:43:11: #2 You may need to consider one of the other alternative d(s): 1,39,598 WARNING @ Tue, 30 Jun 2020 03:43:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:43:11: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:43:11: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:43:20: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:43:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10_peaks.xls INFO @ Tue, 30 Jun 2020 03:43:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:43:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.10_summits.bed INFO @ Tue, 30 Jun 2020 03:43:36: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:43:44: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 03:44:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20_peaks.xls INFO @ Tue, 30 Jun 2020 03:44:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 03:44:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX751567/SRX751567.20_summits.bed INFO @ Tue, 30 Jun 2020 03:44:01: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling