Job ID = 14172896 SRX = SRX7434132 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:16 8208933 reads; of these: 8208933 (100.00%) were unpaired; of these: 545868 (6.65%) aligned 0 times 6150010 (74.92%) aligned exactly 1 time 1513055 (18.43%) aligned >1 times 93.35% overall alignment rate Time searching: 00:02:16 Overall time: 00:02:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 3515143 / 7663065 = 0.4587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:22:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:22:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:22:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:22:26: 1000000 INFO @ Sat, 11 Dec 2021 17:22:32: 2000000 INFO @ Sat, 11 Dec 2021 17:22:37: 3000000 INFO @ Sat, 11 Dec 2021 17:22:43: 4000000 INFO @ Sat, 11 Dec 2021 17:22:43: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:22:43: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:22:43: #1 total tags in treatment: 4147922 INFO @ Sat, 11 Dec 2021 17:22:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:22:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:22:44: #1 tags after filtering in treatment: 4147758 INFO @ Sat, 11 Dec 2021 17:22:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:22:44: #1 finished! INFO @ Sat, 11 Dec 2021 17:22:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:22:44: #2 number of paired peaks: 4086 INFO @ Sat, 11 Dec 2021 17:22:44: start model_add_line... INFO @ Sat, 11 Dec 2021 17:22:44: start X-correlation... INFO @ Sat, 11 Dec 2021 17:22:44: end of X-cor INFO @ Sat, 11 Dec 2021 17:22:44: #2 finished! INFO @ Sat, 11 Dec 2021 17:22:44: #2 predicted fragment length is 241 bps INFO @ Sat, 11 Dec 2021 17:22:44: #2 alternative fragment length(s) may be 241 bps INFO @ Sat, 11 Dec 2021 17:22:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05_model.r INFO @ Sat, 11 Dec 2021 17:22:44: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:22:44: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:22:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:22:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:22:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:22:55: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:22:57: 1000000 INFO @ Sat, 11 Dec 2021 17:23:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05_peaks.xls INFO @ Sat, 11 Dec 2021 17:23:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:23:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.05_summits.bed INFO @ Sat, 11 Dec 2021 17:23:00: Done! pass1 - making usageList (493 chroms): 2 millis pass2 - checking and writing primary data (4102 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:23:04: 2000000 INFO @ Sat, 11 Dec 2021 17:23:10: 3000000 INFO @ Sat, 11 Dec 2021 17:23:17: 4000000 INFO @ Sat, 11 Dec 2021 17:23:18: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:23:18: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:23:18: #1 total tags in treatment: 4147922 INFO @ Sat, 11 Dec 2021 17:23:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:23:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:23:19: #1 tags after filtering in treatment: 4147758 INFO @ Sat, 11 Dec 2021 17:23:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:23:19: #1 finished! INFO @ Sat, 11 Dec 2021 17:23:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:23:19: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:23:19: #2 number of paired peaks: 4086 INFO @ Sat, 11 Dec 2021 17:23:19: start model_add_line... INFO @ Sat, 11 Dec 2021 17:23:19: start X-correlation... INFO @ Sat, 11 Dec 2021 17:23:19: end of X-cor INFO @ Sat, 11 Dec 2021 17:23:19: #2 finished! INFO @ Sat, 11 Dec 2021 17:23:19: #2 predicted fragment length is 241 bps INFO @ Sat, 11 Dec 2021 17:23:19: #2 alternative fragment length(s) may be 241 bps INFO @ Sat, 11 Dec 2021 17:23:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10_model.r INFO @ Sat, 11 Dec 2021 17:23:19: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:23:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:23:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:23:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:23:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:23:28: 1000000 INFO @ Sat, 11 Dec 2021 17:23:30: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:23:34: 2000000 INFO @ Sat, 11 Dec 2021 17:23:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10_peaks.xls INFO @ Sat, 11 Dec 2021 17:23:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:23:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.10_summits.bed INFO @ Sat, 11 Dec 2021 17:23:35: Done! pass1 - making usageList (322 chroms): 2 millis pass2 - checking and writing primary data (3097 records, 4 fields): 11 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 17:23:41: 3000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 17:23:48: 4000000 INFO @ Sat, 11 Dec 2021 17:23:49: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:23:49: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:23:49: #1 total tags in treatment: 4147922 INFO @ Sat, 11 Dec 2021 17:23:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:23:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:23:49: #1 tags after filtering in treatment: 4147758 INFO @ Sat, 11 Dec 2021 17:23:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:23:49: #1 finished! INFO @ Sat, 11 Dec 2021 17:23:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:23:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:23:50: #2 number of paired peaks: 4086 INFO @ Sat, 11 Dec 2021 17:23:50: start model_add_line... INFO @ Sat, 11 Dec 2021 17:23:50: start X-correlation... INFO @ Sat, 11 Dec 2021 17:23:50: end of X-cor INFO @ Sat, 11 Dec 2021 17:23:50: #2 finished! INFO @ Sat, 11 Dec 2021 17:23:50: #2 predicted fragment length is 241 bps INFO @ Sat, 11 Dec 2021 17:23:50: #2 alternative fragment length(s) may be 241 bps INFO @ Sat, 11 Dec 2021 17:23:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20_model.r INFO @ Sat, 11 Dec 2021 17:23:50: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:23:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:24:01: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:24:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20_peaks.xls INFO @ Sat, 11 Dec 2021 17:24:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:24:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434132/SRX7434132.20_summits.bed INFO @ Sat, 11 Dec 2021 17:24:06: Done! pass1 - making usageList (140 chroms): 1 millis pass2 - checking and writing primary data (2379 records, 4 fields): 8 millis CompletedMACS2peakCalling