Job ID = 14172926 SRX = SRX7434130 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:49 33730634 reads; of these: 33730634 (100.00%) were unpaired; of these: 12070862 (35.79%) aligned 0 times 15291931 (45.34%) aligned exactly 1 time 6367841 (18.88%) aligned >1 times 64.21% overall alignment rate Time searching: 00:06:49 Overall time: 00:06:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6422849 / 21659772 = 0.2965 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:36:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:36:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:36:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:36:28: 1000000 INFO @ Sat, 11 Dec 2021 17:36:33: 2000000 INFO @ Sat, 11 Dec 2021 17:36:38: 3000000 INFO @ Sat, 11 Dec 2021 17:36:44: 4000000 INFO @ Sat, 11 Dec 2021 17:36:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:36:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:36:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:36:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:36:54: 6000000 INFO @ Sat, 11 Dec 2021 17:36:58: 1000000 INFO @ Sat, 11 Dec 2021 17:37:00: 7000000 INFO @ Sat, 11 Dec 2021 17:37:03: 2000000 INFO @ Sat, 11 Dec 2021 17:37:05: 8000000 INFO @ Sat, 11 Dec 2021 17:37:09: 3000000 INFO @ Sat, 11 Dec 2021 17:37:10: 9000000 INFO @ Sat, 11 Dec 2021 17:37:14: 4000000 INFO @ Sat, 11 Dec 2021 17:37:16: 10000000 INFO @ Sat, 11 Dec 2021 17:37:20: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:37:21: 11000000 INFO @ Sat, 11 Dec 2021 17:37:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:37:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:37:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:37:25: 6000000 INFO @ Sat, 11 Dec 2021 17:37:27: 12000000 INFO @ Sat, 11 Dec 2021 17:37:28: 1000000 INFO @ Sat, 11 Dec 2021 17:37:30: 7000000 INFO @ Sat, 11 Dec 2021 17:37:33: 13000000 INFO @ Sat, 11 Dec 2021 17:37:33: 2000000 INFO @ Sat, 11 Dec 2021 17:37:36: 8000000 INFO @ Sat, 11 Dec 2021 17:37:38: 14000000 INFO @ Sat, 11 Dec 2021 17:37:39: 3000000 INFO @ Sat, 11 Dec 2021 17:37:41: 9000000 INFO @ Sat, 11 Dec 2021 17:37:44: 15000000 INFO @ Sat, 11 Dec 2021 17:37:44: 4000000 INFO @ Sat, 11 Dec 2021 17:37:45: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:37:45: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:37:45: #1 total tags in treatment: 15236923 INFO @ Sat, 11 Dec 2021 17:37:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:37:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:37:46: #1 tags after filtering in treatment: 15236851 INFO @ Sat, 11 Dec 2021 17:37:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:37:46: #1 finished! INFO @ Sat, 11 Dec 2021 17:37:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:37:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:37:47: #2 number of paired peaks: 1252 INFO @ Sat, 11 Dec 2021 17:37:47: start model_add_line... INFO @ Sat, 11 Dec 2021 17:37:47: 10000000 INFO @ Sat, 11 Dec 2021 17:37:47: start X-correlation... INFO @ Sat, 11 Dec 2021 17:37:47: end of X-cor INFO @ Sat, 11 Dec 2021 17:37:47: #2 finished! INFO @ Sat, 11 Dec 2021 17:37:47: #2 predicted fragment length is 232 bps INFO @ Sat, 11 Dec 2021 17:37:47: #2 alternative fragment length(s) may be 232 bps INFO @ Sat, 11 Dec 2021 17:37:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05_model.r INFO @ Sat, 11 Dec 2021 17:37:47: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:37:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:37:50: 5000000 INFO @ Sat, 11 Dec 2021 17:37:52: 11000000 INFO @ Sat, 11 Dec 2021 17:37:55: 6000000 INFO @ Sat, 11 Dec 2021 17:37:58: 12000000 INFO @ Sat, 11 Dec 2021 17:38:01: 7000000 INFO @ Sat, 11 Dec 2021 17:38:04: 13000000 INFO @ Sat, 11 Dec 2021 17:38:06: 8000000 INFO @ Sat, 11 Dec 2021 17:38:09: 14000000 INFO @ Sat, 11 Dec 2021 17:38:12: 9000000 INFO @ Sat, 11 Dec 2021 17:38:15: 15000000 INFO @ Sat, 11 Dec 2021 17:38:17: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:38:17: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:38:17: #1 total tags in treatment: 15236923 INFO @ Sat, 11 Dec 2021 17:38:17: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:38:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:38:17: #1 tags after filtering in treatment: 15236851 INFO @ Sat, 11 Dec 2021 17:38:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:38:17: #1 finished! INFO @ Sat, 11 Dec 2021 17:38:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:38:17: 10000000 INFO @ Sat, 11 Dec 2021 17:38:18: #2 number of paired peaks: 1252 INFO @ Sat, 11 Dec 2021 17:38:18: start model_add_line... INFO @ Sat, 11 Dec 2021 17:38:18: start X-correlation... INFO @ Sat, 11 Dec 2021 17:38:18: end of X-cor INFO @ Sat, 11 Dec 2021 17:38:18: #2 finished! INFO @ Sat, 11 Dec 2021 17:38:18: #2 predicted fragment length is 232 bps INFO @ Sat, 11 Dec 2021 17:38:18: #2 alternative fragment length(s) may be 232 bps INFO @ Sat, 11 Dec 2021 17:38:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10_model.r INFO @ Sat, 11 Dec 2021 17:38:18: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:38:18: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:38:23: 11000000 INFO @ Sat, 11 Dec 2021 17:38:24: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:38:28: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 17:38:34: 13000000 INFO @ Sat, 11 Dec 2021 17:38:39: 14000000 INFO @ Sat, 11 Dec 2021 17:38:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05_peaks.xls INFO @ Sat, 11 Dec 2021 17:38:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:38:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.05_summits.bed INFO @ Sat, 11 Dec 2021 17:38:41: Done! pass1 - making usageList (741 chroms): 2 millis pass2 - checking and writing primary data (4569 records, 4 fields): 24 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:38:44: 15000000 INFO @ Sat, 11 Dec 2021 17:38:46: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:38:46: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:38:46: #1 total tags in treatment: 15236923 INFO @ Sat, 11 Dec 2021 17:38:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:38:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:38:46: #1 tags after filtering in treatment: 15236851 INFO @ Sat, 11 Dec 2021 17:38:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:38:46: #1 finished! INFO @ Sat, 11 Dec 2021 17:38:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:38:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:38:47: #2 number of paired peaks: 1252 INFO @ Sat, 11 Dec 2021 17:38:47: start model_add_line... INFO @ Sat, 11 Dec 2021 17:38:48: start X-correlation... INFO @ Sat, 11 Dec 2021 17:38:48: end of X-cor INFO @ Sat, 11 Dec 2021 17:38:48: #2 finished! INFO @ Sat, 11 Dec 2021 17:38:48: #2 predicted fragment length is 232 bps INFO @ Sat, 11 Dec 2021 17:38:48: #2 alternative fragment length(s) may be 232 bps INFO @ Sat, 11 Dec 2021 17:38:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20_model.r INFO @ Sat, 11 Dec 2021 17:38:48: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:38:48: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:38:55: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 17:39:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10_peaks.xls INFO @ Sat, 11 Dec 2021 17:39:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:39:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.10_summits.bed INFO @ Sat, 11 Dec 2021 17:39:11: Done! pass1 - making usageList (684 chroms): 1 millis pass2 - checking and writing primary data (3395 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:39:23: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:39:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20_peaks.xls INFO @ Sat, 11 Dec 2021 17:39:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:39:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434130/SRX7434130.20_summits.bed INFO @ Sat, 11 Dec 2021 17:39:39: Done! pass1 - making usageList (542 chroms): 2 millis pass2 - checking and writing primary data (2461 records, 4 fields): 17 millis CompletedMACS2peakCalling