Job ID = 14172850 SRX = SRX7434123 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:58 22361233 reads; of these: 22361233 (100.00%) were unpaired; of these: 2893317 (12.94%) aligned 0 times 15964949 (71.40%) aligned exactly 1 time 3502967 (15.67%) aligned >1 times 87.06% overall alignment rate Time searching: 00:04:58 Overall time: 00:04:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7510224 / 19467916 = 0.3858 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:16:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:16:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:16:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:16:26: 1000000 INFO @ Sat, 11 Dec 2021 17:16:31: 2000000 INFO @ Sat, 11 Dec 2021 17:16:36: 3000000 INFO @ Sat, 11 Dec 2021 17:16:42: 4000000 INFO @ Sat, 11 Dec 2021 17:16:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:16:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:16:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:16:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:16:52: 6000000 INFO @ Sat, 11 Dec 2021 17:16:55: 1000000 INFO @ Sat, 11 Dec 2021 17:16:58: 7000000 INFO @ Sat, 11 Dec 2021 17:17:00: 2000000 INFO @ Sat, 11 Dec 2021 17:17:03: 8000000 INFO @ Sat, 11 Dec 2021 17:17:05: 3000000 INFO @ Sat, 11 Dec 2021 17:17:09: 9000000 INFO @ Sat, 11 Dec 2021 17:17:09: 4000000 INFO @ Sat, 11 Dec 2021 17:17:14: 5000000 INFO @ Sat, 11 Dec 2021 17:17:14: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:17:19: 6000000 INFO @ Sat, 11 Dec 2021 17:17:20: 11000000 INFO @ Sat, 11 Dec 2021 17:17:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:17:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:17:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:17:24: 7000000 INFO @ Sat, 11 Dec 2021 17:17:25: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:17:25: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:17:25: #1 total tags in treatment: 11957692 INFO @ Sat, 11 Dec 2021 17:17:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:17:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:17:26: #1 tags after filtering in treatment: 11957591 INFO @ Sat, 11 Dec 2021 17:17:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:17:26: #1 finished! INFO @ Sat, 11 Dec 2021 17:17:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:17:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:17:26: 1000000 INFO @ Sat, 11 Dec 2021 17:17:27: #2 number of paired peaks: 4134 INFO @ Sat, 11 Dec 2021 17:17:27: start model_add_line... INFO @ Sat, 11 Dec 2021 17:17:27: start X-correlation... INFO @ Sat, 11 Dec 2021 17:17:27: end of X-cor INFO @ Sat, 11 Dec 2021 17:17:27: #2 finished! INFO @ Sat, 11 Dec 2021 17:17:27: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:17:27: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:17:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05_model.r INFO @ Sat, 11 Dec 2021 17:17:27: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:17:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:17:29: 8000000 INFO @ Sat, 11 Dec 2021 17:17:31: 2000000 INFO @ Sat, 11 Dec 2021 17:17:33: 9000000 INFO @ Sat, 11 Dec 2021 17:17:37: 3000000 INFO @ Sat, 11 Dec 2021 17:17:38: 10000000 INFO @ Sat, 11 Dec 2021 17:17:42: 4000000 INFO @ Sat, 11 Dec 2021 17:17:43: 11000000 INFO @ Sat, 11 Dec 2021 17:17:47: 5000000 INFO @ Sat, 11 Dec 2021 17:17:48: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:17:48: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:17:48: #1 total tags in treatment: 11957692 INFO @ Sat, 11 Dec 2021 17:17:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:17:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:17:48: #1 tags after filtering in treatment: 11957591 INFO @ Sat, 11 Dec 2021 17:17:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:17:48: #1 finished! INFO @ Sat, 11 Dec 2021 17:17:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:17:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:17:49: #2 number of paired peaks: 4134 INFO @ Sat, 11 Dec 2021 17:17:49: start model_add_line... INFO @ Sat, 11 Dec 2021 17:17:50: start X-correlation... INFO @ Sat, 11 Dec 2021 17:17:50: end of X-cor INFO @ Sat, 11 Dec 2021 17:17:50: #2 finished! INFO @ Sat, 11 Dec 2021 17:17:50: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:17:50: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:17:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10_model.r INFO @ Sat, 11 Dec 2021 17:17:50: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:17:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:17:53: 6000000 INFO @ Sat, 11 Dec 2021 17:17:58: 7000000 INFO @ Sat, 11 Dec 2021 17:18:00: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:18:03: 8000000 INFO @ Sat, 11 Dec 2021 17:18:08: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 17:18:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05_peaks.xls INFO @ Sat, 11 Dec 2021 17:18:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:18:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.05_summits.bed INFO @ Sat, 11 Dec 2021 17:18:13: Done! pass1 - making usageList (569 chroms): 2 millis pass2 - checking and writing primary data (6275 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:18:14: 10000000 INFO @ Sat, 11 Dec 2021 17:18:19: 11000000 INFO @ Sat, 11 Dec 2021 17:18:22: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:18:25: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 17:18:25: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 17:18:25: #1 total tags in treatment: 11957692 INFO @ Sat, 11 Dec 2021 17:18:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:18:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:18:25: #1 tags after filtering in treatment: 11957591 INFO @ Sat, 11 Dec 2021 17:18:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:18:25: #1 finished! INFO @ Sat, 11 Dec 2021 17:18:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:18:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:18:26: #2 number of paired peaks: 4134 INFO @ Sat, 11 Dec 2021 17:18:26: start model_add_line... INFO @ Sat, 11 Dec 2021 17:18:27: start X-correlation... INFO @ Sat, 11 Dec 2021 17:18:27: end of X-cor INFO @ Sat, 11 Dec 2021 17:18:27: #2 finished! INFO @ Sat, 11 Dec 2021 17:18:27: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:18:27: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:18:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20_model.r INFO @ Sat, 11 Dec 2021 17:18:27: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:18:27: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 17:18:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10_peaks.xls INFO @ Sat, 11 Dec 2021 17:18:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:18:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.10_summits.bed INFO @ Sat, 11 Dec 2021 17:18:35: Done! pass1 - making usageList (416 chroms): 2 millis pass2 - checking and writing primary data (5138 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:19:00: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:19:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20_peaks.xls INFO @ Sat, 11 Dec 2021 17:19:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:19:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7434123/SRX7434123.20_summits.bed INFO @ Sat, 11 Dec 2021 17:19:14: Done! pass1 - making usageList (223 chroms): 1 millis pass2 - checking and writing primary data (4121 records, 4 fields): 10 millis CompletedMACS2peakCalling