Job ID = 6626780 SRX = SRX7351005 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:42 18872202 reads; of these: 18872202 (100.00%) were unpaired; of these: 638758 (3.38%) aligned 0 times 15003651 (79.50%) aligned exactly 1 time 3229793 (17.11%) aligned >1 times 96.62% overall alignment rate Time searching: 00:06:43 Overall time: 00:06:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4588485 / 18233444 = 0.2517 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:55:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:55:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:55:39: 1000000 INFO @ Tue, 14 Jul 2020 08:55:47: 2000000 INFO @ Tue, 14 Jul 2020 08:55:55: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:56:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:56:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:56:02: 4000000 INFO @ Tue, 14 Jul 2020 08:56:10: 5000000 INFO @ Tue, 14 Jul 2020 08:56:11: 1000000 INFO @ Tue, 14 Jul 2020 08:56:19: 6000000 INFO @ Tue, 14 Jul 2020 08:56:20: 2000000 INFO @ Tue, 14 Jul 2020 08:56:27: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:56:30: 3000000 INFO @ Tue, 14 Jul 2020 08:56:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:56:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:56:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:56:36: 8000000 INFO @ Tue, 14 Jul 2020 08:56:40: 4000000 INFO @ Tue, 14 Jul 2020 08:56:40: 1000000 INFO @ Tue, 14 Jul 2020 08:56:45: 9000000 INFO @ Tue, 14 Jul 2020 08:56:49: 2000000 INFO @ Tue, 14 Jul 2020 08:56:50: 5000000 INFO @ Tue, 14 Jul 2020 08:56:54: 10000000 INFO @ Tue, 14 Jul 2020 08:56:58: 3000000 INFO @ Tue, 14 Jul 2020 08:56:59: 6000000 INFO @ Tue, 14 Jul 2020 08:57:02: 11000000 INFO @ Tue, 14 Jul 2020 08:57:07: 4000000 INFO @ Tue, 14 Jul 2020 08:57:08: 7000000 INFO @ Tue, 14 Jul 2020 08:57:11: 12000000 INFO @ Tue, 14 Jul 2020 08:57:16: 5000000 INFO @ Tue, 14 Jul 2020 08:57:18: 8000000 INFO @ Tue, 14 Jul 2020 08:57:20: 13000000 INFO @ Tue, 14 Jul 2020 08:57:25: 6000000 INFO @ Tue, 14 Jul 2020 08:57:26: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:57:26: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:57:26: #1 total tags in treatment: 13644959 INFO @ Tue, 14 Jul 2020 08:57:26: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:26: #1 tags after filtering in treatment: 13644726 INFO @ Tue, 14 Jul 2020 08:57:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:57:26: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:26: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:57:27: 9000000 INFO @ Tue, 14 Jul 2020 08:57:28: #2 number of paired peaks: 8686 INFO @ Tue, 14 Jul 2020 08:57:28: start model_add_line... INFO @ Tue, 14 Jul 2020 08:57:28: start X-correlation... INFO @ Tue, 14 Jul 2020 08:57:28: end of X-cor INFO @ Tue, 14 Jul 2020 08:57:28: #2 finished! INFO @ Tue, 14 Jul 2020 08:57:28: #2 predicted fragment length is 151 bps INFO @ Tue, 14 Jul 2020 08:57:28: #2 alternative fragment length(s) may be 151 bps INFO @ Tue, 14 Jul 2020 08:57:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05_model.r INFO @ Tue, 14 Jul 2020 08:57:28: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:57:28: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:57:33: 7000000 INFO @ Tue, 14 Jul 2020 08:57:37: 10000000 INFO @ Tue, 14 Jul 2020 08:57:42: 8000000 INFO @ Tue, 14 Jul 2020 08:57:46: 11000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:57:51: 9000000 INFO @ Tue, 14 Jul 2020 08:57:56: 12000000 INFO @ Tue, 14 Jul 2020 08:57:59: 10000000 INFO @ Tue, 14 Jul 2020 08:58:05: 13000000 INFO @ Tue, 14 Jul 2020 08:58:06: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:58:08: 11000000 INFO @ Tue, 14 Jul 2020 08:58:11: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:58:11: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:58:11: #1 total tags in treatment: 13644959 INFO @ Tue, 14 Jul 2020 08:58:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:58:11: #1 tags after filtering in treatment: 13644726 INFO @ Tue, 14 Jul 2020 08:58:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:58:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:58:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:58:13: #2 number of paired peaks: 8686 INFO @ Tue, 14 Jul 2020 08:58:13: start model_add_line... INFO @ Tue, 14 Jul 2020 08:58:13: start X-correlation... INFO @ Tue, 14 Jul 2020 08:58:13: end of X-cor INFO @ Tue, 14 Jul 2020 08:58:13: #2 finished! INFO @ Tue, 14 Jul 2020 08:58:13: #2 predicted fragment length is 151 bps INFO @ Tue, 14 Jul 2020 08:58:13: #2 alternative fragment length(s) may be 151 bps INFO @ Tue, 14 Jul 2020 08:58:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10_model.r INFO @ Tue, 14 Jul 2020 08:58:13: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:58:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:58:16: 12000000 INFO @ Tue, 14 Jul 2020 08:58:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05_peaks.xls INFO @ Tue, 14 Jul 2020 08:58:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:58:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.05_summits.bed INFO @ Tue, 14 Jul 2020 08:58:22: Done! pass1 - making usageList (198 chroms): 3 millis pass2 - checking and writing primary data (13228 records, 4 fields): 18 millis INFO @ Tue, 14 Jul 2020 08:58:23: 13000000 CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:58:27: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:58:27: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:58:27: #1 total tags in treatment: 13644959 INFO @ Tue, 14 Jul 2020 08:58:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:58:28: #1 tags after filtering in treatment: 13644726 INFO @ Tue, 14 Jul 2020 08:58:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:58:28: #1 finished! INFO @ Tue, 14 Jul 2020 08:58:28: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:58:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:58:29: #2 number of paired peaks: 8686 INFO @ Tue, 14 Jul 2020 08:58:29: start model_add_line... INFO @ Tue, 14 Jul 2020 08:58:29: start X-correlation... INFO @ Tue, 14 Jul 2020 08:58:29: end of X-cor INFO @ Tue, 14 Jul 2020 08:58:29: #2 finished! INFO @ Tue, 14 Jul 2020 08:58:29: #2 predicted fragment length is 151 bps INFO @ Tue, 14 Jul 2020 08:58:29: #2 alternative fragment length(s) may be 151 bps INFO @ Tue, 14 Jul 2020 08:58:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20_model.r INFO @ Tue, 14 Jul 2020 08:58:29: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:58:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:58:53: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:59:07: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:59:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10_peaks.xls INFO @ Tue, 14 Jul 2020 08:59:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:59:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.10_summits.bed INFO @ Tue, 14 Jul 2020 08:59:11: Done! pass1 - making usageList (174 chroms): 2 millis pass2 - checking and writing primary data (10830 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:59:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20_peaks.xls INFO @ Tue, 14 Jul 2020 08:59:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:59:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7351005/SRX7351005.20_summits.bed INFO @ Tue, 14 Jul 2020 08:59:23: Done! pass1 - making usageList (138 chroms): 1 millis pass2 - checking and writing primary data (7326 records, 4 fields): 12 millis CompletedMACS2peakCalling