Job ID = 14171084 SRX = SRX7320033 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:45 76115177 reads; of these: 76115177 (100.00%) were unpaired; of these: 2770636 (3.64%) aligned 0 times 61500246 (80.80%) aligned exactly 1 time 11844295 (15.56%) aligned >1 times 96.36% overall alignment rate Time searching: 00:21:45 Overall time: 00:21:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 19900959 / 73344541 = 0.2713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:07:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:07:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:07:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:07:50: 1000000 INFO @ Sat, 11 Dec 2021 10:08:00: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:08:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:08:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:08:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:08:10: 3000000 INFO @ Sat, 11 Dec 2021 10:08:20: 1000000 INFO @ Sat, 11 Dec 2021 10:08:20: 4000000 INFO @ Sat, 11 Dec 2021 10:08:30: 2000000 INFO @ Sat, 11 Dec 2021 10:08:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:08:40: 3000000 INFO @ Sat, 11 Dec 2021 10:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:08:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:08:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:08:40: 6000000 INFO @ Sat, 11 Dec 2021 10:08:49: 1000000 INFO @ Sat, 11 Dec 2021 10:08:50: 4000000 INFO @ Sat, 11 Dec 2021 10:08:50: 7000000 INFO @ Sat, 11 Dec 2021 10:08:58: 2000000 INFO @ Sat, 11 Dec 2021 10:09:00: 5000000 INFO @ Sat, 11 Dec 2021 10:09:01: 8000000 INFO @ Sat, 11 Dec 2021 10:09:07: 3000000 INFO @ Sat, 11 Dec 2021 10:09:10: 6000000 INFO @ Sat, 11 Dec 2021 10:09:11: 9000000 INFO @ Sat, 11 Dec 2021 10:09:15: 4000000 INFO @ Sat, 11 Dec 2021 10:09:20: 7000000 INFO @ Sat, 11 Dec 2021 10:09:21: 10000000 INFO @ Sat, 11 Dec 2021 10:09:24: 5000000 INFO @ Sat, 11 Dec 2021 10:09:30: 8000000 INFO @ Sat, 11 Dec 2021 10:09:31: 11000000 INFO @ Sat, 11 Dec 2021 10:09:33: 6000000 INFO @ Sat, 11 Dec 2021 10:09:40: 9000000 INFO @ Sat, 11 Dec 2021 10:09:41: 12000000 INFO @ Sat, 11 Dec 2021 10:09:42: 7000000 INFO @ Sat, 11 Dec 2021 10:09:50: 10000000 INFO @ Sat, 11 Dec 2021 10:09:50: 8000000 INFO @ Sat, 11 Dec 2021 10:09:51: 13000000 INFO @ Sat, 11 Dec 2021 10:09:59: 9000000 INFO @ Sat, 11 Dec 2021 10:09:59: 11000000 INFO @ Sat, 11 Dec 2021 10:10:01: 14000000 INFO @ Sat, 11 Dec 2021 10:10:07: 10000000 INFO @ Sat, 11 Dec 2021 10:10:09: 12000000 INFO @ Sat, 11 Dec 2021 10:10:11: 15000000 INFO @ Sat, 11 Dec 2021 10:10:16: 11000000 INFO @ Sat, 11 Dec 2021 10:10:19: 13000000 INFO @ Sat, 11 Dec 2021 10:10:21: 16000000 INFO @ Sat, 11 Dec 2021 10:10:24: 12000000 INFO @ Sat, 11 Dec 2021 10:10:29: 14000000 INFO @ Sat, 11 Dec 2021 10:10:31: 17000000 INFO @ Sat, 11 Dec 2021 10:10:33: 13000000 INFO @ Sat, 11 Dec 2021 10:10:38: 15000000 INFO @ Sat, 11 Dec 2021 10:10:41: 18000000 INFO @ Sat, 11 Dec 2021 10:10:42: 14000000 INFO @ Sat, 11 Dec 2021 10:10:48: 16000000 INFO @ Sat, 11 Dec 2021 10:10:50: 15000000 INFO @ Sat, 11 Dec 2021 10:10:51: 19000000 INFO @ Sat, 11 Dec 2021 10:10:58: 17000000 INFO @ Sat, 11 Dec 2021 10:10:59: 16000000 INFO @ Sat, 11 Dec 2021 10:11:01: 20000000 INFO @ Sat, 11 Dec 2021 10:11:08: 18000000 INFO @ Sat, 11 Dec 2021 10:11:08: 17000000 INFO @ Sat, 11 Dec 2021 10:11:11: 21000000 INFO @ Sat, 11 Dec 2021 10:11:16: 18000000 INFO @ Sat, 11 Dec 2021 10:11:17: 19000000 INFO @ Sat, 11 Dec 2021 10:11:21: 22000000 INFO @ Sat, 11 Dec 2021 10:11:25: 19000000 INFO @ Sat, 11 Dec 2021 10:11:27: 20000000 INFO @ Sat, 11 Dec 2021 10:11:31: 23000000 INFO @ Sat, 11 Dec 2021 10:11:33: 20000000 INFO @ Sat, 11 Dec 2021 10:11:37: 21000000 INFO @ Sat, 11 Dec 2021 10:11:41: 24000000 INFO @ Sat, 11 Dec 2021 10:11:42: 21000000 INFO @ Sat, 11 Dec 2021 10:11:47: 22000000 INFO @ Sat, 11 Dec 2021 10:11:51: 22000000 INFO @ Sat, 11 Dec 2021 10:11:51: 25000000 INFO @ Sat, 11 Dec 2021 10:11:56: 23000000 INFO @ Sat, 11 Dec 2021 10:11:59: 23000000 INFO @ Sat, 11 Dec 2021 10:12:01: 26000000 INFO @ Sat, 11 Dec 2021 10:12:06: 24000000 INFO @ Sat, 11 Dec 2021 10:12:08: 24000000 INFO @ Sat, 11 Dec 2021 10:12:11: 27000000 INFO @ Sat, 11 Dec 2021 10:12:15: 25000000 INFO @ Sat, 11 Dec 2021 10:12:16: 25000000 INFO @ Sat, 11 Dec 2021 10:12:20: 28000000 INFO @ Sat, 11 Dec 2021 10:12:25: 26000000 INFO @ Sat, 11 Dec 2021 10:12:25: 26000000 INFO @ Sat, 11 Dec 2021 10:12:31: 29000000 INFO @ Sat, 11 Dec 2021 10:12:33: 27000000 INFO @ Sat, 11 Dec 2021 10:12:35: 27000000 INFO @ Sat, 11 Dec 2021 10:12:41: 30000000 INFO @ Sat, 11 Dec 2021 10:12:42: 28000000 INFO @ Sat, 11 Dec 2021 10:12:45: 28000000 INFO @ Sat, 11 Dec 2021 10:12:50: 29000000 INFO @ Sat, 11 Dec 2021 10:12:51: 31000000 INFO @ Sat, 11 Dec 2021 10:12:55: 29000000 INFO @ Sat, 11 Dec 2021 10:12:59: 30000000 INFO @ Sat, 11 Dec 2021 10:13:00: 32000000 INFO @ Sat, 11 Dec 2021 10:13:04: 30000000 INFO @ Sat, 11 Dec 2021 10:13:07: 31000000 INFO @ Sat, 11 Dec 2021 10:13:10: 33000000 INFO @ Sat, 11 Dec 2021 10:13:14: 31000000 INFO @ Sat, 11 Dec 2021 10:13:16: 32000000 INFO @ Sat, 11 Dec 2021 10:13:20: 34000000 INFO @ Sat, 11 Dec 2021 10:13:23: 32000000 INFO @ Sat, 11 Dec 2021 10:13:24: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:13:30: 35000000 INFO @ Sat, 11 Dec 2021 10:13:33: 34000000 INFO @ Sat, 11 Dec 2021 10:13:33: 33000000 INFO @ Sat, 11 Dec 2021 10:13:40: 36000000 INFO @ Sat, 11 Dec 2021 10:13:41: 35000000 INFO @ Sat, 11 Dec 2021 10:13:43: 34000000 INFO @ Sat, 11 Dec 2021 10:13:50: 36000000 INFO @ Sat, 11 Dec 2021 10:13:50: 37000000 INFO @ Sat, 11 Dec 2021 10:13:52: 35000000 INFO @ Sat, 11 Dec 2021 10:13:58: 37000000 INFO @ Sat, 11 Dec 2021 10:14:00: 38000000 INFO @ Sat, 11 Dec 2021 10:14:02: 36000000 INFO @ Sat, 11 Dec 2021 10:14:07: 38000000 INFO @ Sat, 11 Dec 2021 10:14:09: 39000000 INFO @ Sat, 11 Dec 2021 10:14:11: 37000000 INFO @ Sat, 11 Dec 2021 10:14:15: 39000000 INFO @ Sat, 11 Dec 2021 10:14:20: 40000000 INFO @ Sat, 11 Dec 2021 10:14:21: 38000000 INFO @ Sat, 11 Dec 2021 10:14:24: 40000000 INFO @ Sat, 11 Dec 2021 10:14:30: 41000000 INFO @ Sat, 11 Dec 2021 10:14:30: 39000000 INFO @ Sat, 11 Dec 2021 10:14:33: 41000000 INFO @ Sat, 11 Dec 2021 10:14:40: 40000000 INFO @ Sat, 11 Dec 2021 10:14:41: 42000000 INFO @ Sat, 11 Dec 2021 10:14:42: 42000000 INFO @ Sat, 11 Dec 2021 10:14:50: 41000000 INFO @ Sat, 11 Dec 2021 10:14:50: 43000000 INFO @ Sat, 11 Dec 2021 10:14:51: 43000000 INFO @ Sat, 11 Dec 2021 10:15:00: 44000000 INFO @ Sat, 11 Dec 2021 10:15:01: 42000000 INFO @ Sat, 11 Dec 2021 10:15:02: 44000000 INFO @ Sat, 11 Dec 2021 10:15:08: 45000000 INFO @ Sat, 11 Dec 2021 10:15:10: 43000000 INFO @ Sat, 11 Dec 2021 10:15:11: 45000000 INFO @ Sat, 11 Dec 2021 10:15:17: 46000000 INFO @ Sat, 11 Dec 2021 10:15:20: 44000000 INFO @ Sat, 11 Dec 2021 10:15:21: 46000000 INFO @ Sat, 11 Dec 2021 10:15:26: 47000000 INFO @ Sat, 11 Dec 2021 10:15:30: 45000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:15:31: 47000000 INFO @ Sat, 11 Dec 2021 10:15:34: 48000000 INFO @ Sat, 11 Dec 2021 10:15:39: 46000000 INFO @ Sat, 11 Dec 2021 10:15:41: 48000000 INFO @ Sat, 11 Dec 2021 10:15:43: 49000000 INFO @ Sat, 11 Dec 2021 10:15:49: 47000000 INFO @ Sat, 11 Dec 2021 10:15:51: 49000000 INFO @ Sat, 11 Dec 2021 10:15:52: 50000000 INFO @ Sat, 11 Dec 2021 10:15:59: 48000000 INFO @ Sat, 11 Dec 2021 10:16:01: 50000000 INFO @ Sat, 11 Dec 2021 10:16:01: 51000000 INFO @ Sat, 11 Dec 2021 10:16:09: 49000000 INFO @ Sat, 11 Dec 2021 10:16:10: 52000000 INFO @ Sat, 11 Dec 2021 10:16:11: 51000000 INFO @ Sat, 11 Dec 2021 10:16:19: 50000000 INFO @ Sat, 11 Dec 2021 10:16:19: 53000000 INFO @ Sat, 11 Dec 2021 10:16:21: 52000000 INFO @ Sat, 11 Dec 2021 10:16:23: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:16:23: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:16:23: #1 total tags in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:16:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:16:25: #1 tags after filtering in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:16:25: #1 finished! INFO @ Sat, 11 Dec 2021 10:16:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:16:29: 51000000 INFO @ Sat, 11 Dec 2021 10:16:29: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:16:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:16:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:16:31: 53000000 INFO @ Sat, 11 Dec 2021 10:16:36: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:16:36: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:16:36: #1 total tags in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:36: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:16:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:16:37: #1 tags after filtering in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:16:37: #1 finished! INFO @ Sat, 11 Dec 2021 10:16:37: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:16:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:16:39: 52000000 INFO @ Sat, 11 Dec 2021 10:16:42: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:16:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:16:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:16:48: 53000000 INFO @ Sat, 11 Dec 2021 10:16:53: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:16:53: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:16:53: #1 total tags in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:16:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:16:54: #1 tags after filtering in treatment: 53443582 INFO @ Sat, 11 Dec 2021 10:16:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:16:54: #1 finished! INFO @ Sat, 11 Dec 2021 10:16:54: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:16:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:16:59: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:16:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:16:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320033/SRX7320033.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling