Job ID = 14171093 SRX = SRX7320031 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:06 91309452 reads; of these: 91309452 (100.00%) were unpaired; of these: 3094783 (3.39%) aligned 0 times 75209487 (82.37%) aligned exactly 1 time 13005182 (14.24%) aligned >1 times 96.61% overall alignment rate Time searching: 00:25:06 Overall time: 00:25:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 28908275 / 88214669 = 0.3277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:17:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:17:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:17:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:17:16: 1000000 INFO @ Sat, 11 Dec 2021 10:17:25: 2000000 INFO @ Sat, 11 Dec 2021 10:17:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:17:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:17:38: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:17:38: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:17:43: 4000000 INFO @ Sat, 11 Dec 2021 10:17:48: 1000000 INFO @ Sat, 11 Dec 2021 10:17:52: 5000000 INFO @ Sat, 11 Dec 2021 10:17:58: 2000000 INFO @ Sat, 11 Dec 2021 10:18:03: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:18:08: 3000000 INFO @ Sat, 11 Dec 2021 10:18:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:18:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:18:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:18:12: 7000000 INFO @ Sat, 11 Dec 2021 10:18:17: 4000000 INFO @ Sat, 11 Dec 2021 10:18:18: 1000000 INFO @ Sat, 11 Dec 2021 10:18:22: 8000000 INFO @ Sat, 11 Dec 2021 10:18:26: 5000000 INFO @ Sat, 11 Dec 2021 10:18:28: 2000000 INFO @ Sat, 11 Dec 2021 10:18:31: 9000000 INFO @ Sat, 11 Dec 2021 10:18:35: 6000000 INFO @ Sat, 11 Dec 2021 10:18:36: 3000000 INFO @ Sat, 11 Dec 2021 10:18:39: 10000000 INFO @ Sat, 11 Dec 2021 10:18:43: 7000000 INFO @ Sat, 11 Dec 2021 10:18:44: 4000000 INFO @ Sat, 11 Dec 2021 10:18:48: 11000000 INFO @ Sat, 11 Dec 2021 10:18:51: 8000000 INFO @ Sat, 11 Dec 2021 10:18:53: 5000000 INFO @ Sat, 11 Dec 2021 10:18:56: 12000000 INFO @ Sat, 11 Dec 2021 10:19:00: 9000000 INFO @ Sat, 11 Dec 2021 10:19:02: 6000000 INFO @ Sat, 11 Dec 2021 10:19:04: 13000000 INFO @ Sat, 11 Dec 2021 10:19:09: 10000000 INFO @ Sat, 11 Dec 2021 10:19:11: 7000000 INFO @ Sat, 11 Dec 2021 10:19:12: 14000000 INFO @ Sat, 11 Dec 2021 10:19:18: 11000000 INFO @ Sat, 11 Dec 2021 10:19:20: 15000000 INFO @ Sat, 11 Dec 2021 10:19:20: 8000000 INFO @ Sat, 11 Dec 2021 10:19:27: 12000000 INFO @ Sat, 11 Dec 2021 10:19:28: 16000000 INFO @ Sat, 11 Dec 2021 10:19:30: 9000000 INFO @ Sat, 11 Dec 2021 10:19:35: 13000000 INFO @ Sat, 11 Dec 2021 10:19:38: 17000000 INFO @ Sat, 11 Dec 2021 10:19:41: 10000000 INFO @ Sat, 11 Dec 2021 10:19:44: 14000000 INFO @ Sat, 11 Dec 2021 10:19:48: 18000000 INFO @ Sat, 11 Dec 2021 10:19:51: 11000000 INFO @ Sat, 11 Dec 2021 10:19:52: 15000000 INFO @ Sat, 11 Dec 2021 10:19:57: 19000000 INFO @ Sat, 11 Dec 2021 10:20:00: 12000000 INFO @ Sat, 11 Dec 2021 10:20:01: 16000000 INFO @ Sat, 11 Dec 2021 10:20:07: 20000000 INFO @ Sat, 11 Dec 2021 10:20:09: 17000000 INFO @ Sat, 11 Dec 2021 10:20:09: 13000000 INFO @ Sat, 11 Dec 2021 10:20:17: 21000000 INFO @ Sat, 11 Dec 2021 10:20:17: 18000000 INFO @ Sat, 11 Dec 2021 10:20:19: 14000000 INFO @ Sat, 11 Dec 2021 10:20:25: 19000000 INFO @ Sat, 11 Dec 2021 10:20:27: 22000000 INFO @ Sat, 11 Dec 2021 10:20:29: 15000000 INFO @ Sat, 11 Dec 2021 10:20:33: 20000000 INFO @ Sat, 11 Dec 2021 10:20:36: 23000000 INFO @ Sat, 11 Dec 2021 10:20:39: 16000000 INFO @ Sat, 11 Dec 2021 10:20:41: 21000000 INFO @ Sat, 11 Dec 2021 10:20:46: 24000000 INFO @ Sat, 11 Dec 2021 10:20:48: 17000000 INFO @ Sat, 11 Dec 2021 10:20:50: 22000000 INFO @ Sat, 11 Dec 2021 10:20:55: 25000000 INFO @ Sat, 11 Dec 2021 10:20:56: 18000000 INFO @ Sat, 11 Dec 2021 10:20:58: 23000000 INFO @ Sat, 11 Dec 2021 10:21:04: 26000000 INFO @ Sat, 11 Dec 2021 10:21:05: 19000000 INFO @ Sat, 11 Dec 2021 10:21:06: 24000000 INFO @ Sat, 11 Dec 2021 10:21:13: 27000000 INFO @ Sat, 11 Dec 2021 10:21:13: 25000000 INFO @ Sat, 11 Dec 2021 10:21:13: 20000000 INFO @ Sat, 11 Dec 2021 10:21:21: 26000000 INFO @ Sat, 11 Dec 2021 10:21:22: 21000000 INFO @ Sat, 11 Dec 2021 10:21:22: 28000000 INFO @ Sat, 11 Dec 2021 10:21:29: 27000000 INFO @ Sat, 11 Dec 2021 10:21:32: 29000000 INFO @ Sat, 11 Dec 2021 10:21:32: 22000000 INFO @ Sat, 11 Dec 2021 10:21:37: 28000000 INFO @ Sat, 11 Dec 2021 10:21:41: 23000000 INFO @ Sat, 11 Dec 2021 10:21:41: 30000000 INFO @ Sat, 11 Dec 2021 10:21:45: 29000000 INFO @ Sat, 11 Dec 2021 10:21:50: 24000000 INFO @ Sat, 11 Dec 2021 10:21:51: 31000000 INFO @ Sat, 11 Dec 2021 10:21:53: 30000000 INFO @ Sat, 11 Dec 2021 10:21:58: 25000000 INFO @ Sat, 11 Dec 2021 10:22:00: 32000000 INFO @ Sat, 11 Dec 2021 10:22:01: 31000000 INFO @ Sat, 11 Dec 2021 10:22:06: 26000000 INFO @ Sat, 11 Dec 2021 10:22:09: 32000000 INFO @ Sat, 11 Dec 2021 10:22:10: 33000000 INFO @ Sat, 11 Dec 2021 10:22:14: 27000000 INFO @ Sat, 11 Dec 2021 10:22:17: 33000000 INFO @ Sat, 11 Dec 2021 10:22:20: 34000000 INFO @ Sat, 11 Dec 2021 10:22:22: 28000000 INFO @ Sat, 11 Dec 2021 10:22:25: 34000000 INFO @ Sat, 11 Dec 2021 10:22:29: 35000000 INFO @ Sat, 11 Dec 2021 10:22:30: 29000000 INFO @ Sat, 11 Dec 2021 10:22:33: 35000000 INFO @ Sat, 11 Dec 2021 10:22:38: 30000000 INFO @ Sat, 11 Dec 2021 10:22:39: 36000000 INFO @ Sat, 11 Dec 2021 10:22:41: 36000000 INFO @ Sat, 11 Dec 2021 10:22:46: 31000000 INFO @ Sat, 11 Dec 2021 10:22:48: 37000000 INFO @ Sat, 11 Dec 2021 10:22:49: 37000000 INFO @ Sat, 11 Dec 2021 10:22:54: 32000000 INFO @ Sat, 11 Dec 2021 10:22:57: 38000000 INFO @ Sat, 11 Dec 2021 10:22:57: 38000000 INFO @ Sat, 11 Dec 2021 10:23:02: 33000000 INFO @ Sat, 11 Dec 2021 10:23:05: 39000000 INFO @ Sat, 11 Dec 2021 10:23:06: 39000000 INFO @ Sat, 11 Dec 2021 10:23:10: 34000000 INFO @ Sat, 11 Dec 2021 10:23:13: 40000000 INFO @ Sat, 11 Dec 2021 10:23:15: 40000000 INFO @ Sat, 11 Dec 2021 10:23:18: 35000000 INFO @ Sat, 11 Dec 2021 10:23:21: 41000000 INFO @ Sat, 11 Dec 2021 10:23:24: 41000000 INFO @ Sat, 11 Dec 2021 10:23:26: 36000000 INFO @ Sat, 11 Dec 2021 10:23:29: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:23:33: 42000000 INFO @ Sat, 11 Dec 2021 10:23:34: 37000000 INFO @ Sat, 11 Dec 2021 10:23:37: 43000000 INFO @ Sat, 11 Dec 2021 10:23:41: 38000000 INFO @ Sat, 11 Dec 2021 10:23:42: 43000000 INFO @ Sat, 11 Dec 2021 10:23:45: 44000000 INFO @ Sat, 11 Dec 2021 10:23:49: 39000000 INFO @ Sat, 11 Dec 2021 10:23:51: 44000000 INFO @ Sat, 11 Dec 2021 10:23:53: 45000000 INFO @ Sat, 11 Dec 2021 10:23:57: 40000000 INFO @ Sat, 11 Dec 2021 10:24:01: 46000000 INFO @ Sat, 11 Dec 2021 10:24:01: 45000000 INFO @ Sat, 11 Dec 2021 10:24:05: 41000000 INFO @ Sat, 11 Dec 2021 10:24:08: 47000000 INFO @ Sat, 11 Dec 2021 10:24:10: 46000000 INFO @ Sat, 11 Dec 2021 10:24:12: 42000000 INFO @ Sat, 11 Dec 2021 10:24:16: 48000000 INFO @ Sat, 11 Dec 2021 10:24:19: 47000000 INFO @ Sat, 11 Dec 2021 10:24:21: 43000000 INFO @ Sat, 11 Dec 2021 10:24:25: 49000000 INFO @ Sat, 11 Dec 2021 10:24:28: 48000000 INFO @ Sat, 11 Dec 2021 10:24:29: 44000000 INFO @ Sat, 11 Dec 2021 10:24:33: 50000000 INFO @ Sat, 11 Dec 2021 10:24:38: 45000000 INFO @ Sat, 11 Dec 2021 10:24:39: 49000000 INFO @ Sat, 11 Dec 2021 10:24:41: 51000000 INFO @ Sat, 11 Dec 2021 10:24:47: 46000000 INFO @ Sat, 11 Dec 2021 10:24:48: 50000000 INFO @ Sat, 11 Dec 2021 10:24:49: 52000000 INFO @ Sat, 11 Dec 2021 10:24:55: 47000000 INFO @ Sat, 11 Dec 2021 10:24:57: 51000000 INFO @ Sat, 11 Dec 2021 10:24:57: 53000000 INFO @ Sat, 11 Dec 2021 10:25:04: 48000000 INFO @ Sat, 11 Dec 2021 10:25:04: 54000000 INFO @ Sat, 11 Dec 2021 10:25:06: 52000000 INFO @ Sat, 11 Dec 2021 10:25:12: 55000000 INFO @ Sat, 11 Dec 2021 10:25:14: 49000000 INFO @ Sat, 11 Dec 2021 10:25:15: 53000000 INFO @ Sat, 11 Dec 2021 10:25:19: 56000000 INFO @ Sat, 11 Dec 2021 10:25:23: 50000000 INFO @ Sat, 11 Dec 2021 10:25:25: 54000000 INFO @ Sat, 11 Dec 2021 10:25:27: 57000000 INFO @ Sat, 11 Dec 2021 10:25:31: 51000000 INFO @ Sat, 11 Dec 2021 10:25:34: 55000000 INFO @ Sat, 11 Dec 2021 10:25:34: 58000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:25:40: 52000000 INFO @ Sat, 11 Dec 2021 10:25:42: 59000000 INFO @ Sat, 11 Dec 2021 10:25:43: 56000000 INFO @ Sat, 11 Dec 2021 10:25:45: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:25:45: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:25:45: #1 total tags in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:25:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:25:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:25:47: #1 tags after filtering in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:25:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:25:47: #1 finished! INFO @ Sat, 11 Dec 2021 10:25:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:25:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:25:49: 53000000 INFO @ Sat, 11 Dec 2021 10:25:51: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:25:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:25:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:25:52: 57000000 INFO @ Sat, 11 Dec 2021 10:25:58: 54000000 INFO @ Sat, 11 Dec 2021 10:26:01: 58000000 INFO @ Sat, 11 Dec 2021 10:26:06: 55000000 INFO @ Sat, 11 Dec 2021 10:26:10: 59000000 INFO @ Sat, 11 Dec 2021 10:26:14: 56000000 INFO @ Sat, 11 Dec 2021 10:26:14: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:26:14: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:26:14: #1 total tags in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:26:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:26:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:26:16: #1 tags after filtering in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:26:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:26:16: #1 finished! INFO @ Sat, 11 Dec 2021 10:26:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:26:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:26:20: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:26:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:26:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:26:21: 57000000 INFO @ Sat, 11 Dec 2021 10:26:29: 58000000 INFO @ Sat, 11 Dec 2021 10:26:37: 59000000 INFO @ Sat, 11 Dec 2021 10:26:40: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 10:26:40: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 10:26:40: #1 total tags in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:26:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:26:41: #1 tags after filtering in treatment: 59306394 INFO @ Sat, 11 Dec 2021 10:26:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:26:41: #1 finished! INFO @ Sat, 11 Dec 2021 10:26:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:26:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:26:46: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 10:26:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:26:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7320031/SRX7320031.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling