Job ID = 12266834 SRX = SRX7282932 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:25 21324348 reads; of these: 21324348 (100.00%) were unpaired; of these: 2960521 (13.88%) aligned 0 times 16811995 (78.84%) aligned exactly 1 time 1551832 (7.28%) aligned >1 times 86.12% overall alignment rate Time searching: 00:04:25 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10542109 / 18363827 = 0.5741 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:33:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:33:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:33:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:33:12: 1000000 INFO @ Sat, 03 Apr 2021 09:33:18: 2000000 INFO @ Sat, 03 Apr 2021 09:33:24: 3000000 INFO @ Sat, 03 Apr 2021 09:33:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:33:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:33:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:33:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:33:36: 5000000 INFO @ Sat, 03 Apr 2021 09:33:42: 6000000 INFO @ Sat, 03 Apr 2021 09:33:42: 1000000 INFO @ Sat, 03 Apr 2021 09:33:49: 2000000 INFO @ Sat, 03 Apr 2021 09:33:49: 7000000 INFO @ Sat, 03 Apr 2021 09:33:54: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:33:54: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:33:54: #1 total tags in treatment: 7821718 INFO @ Sat, 03 Apr 2021 09:33:54: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:33:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:33:55: #1 tags after filtering in treatment: 7821605 INFO @ Sat, 03 Apr 2021 09:33:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:33:55: #1 finished! INFO @ Sat, 03 Apr 2021 09:33:55: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:33:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:33:55: 3000000 INFO @ Sat, 03 Apr 2021 09:33:56: #2 number of paired peaks: 5636 INFO @ Sat, 03 Apr 2021 09:33:56: start model_add_line... INFO @ Sat, 03 Apr 2021 09:33:56: start X-correlation... INFO @ Sat, 03 Apr 2021 09:33:56: end of X-cor INFO @ Sat, 03 Apr 2021 09:33:56: #2 finished! INFO @ Sat, 03 Apr 2021 09:33:56: #2 predicted fragment length is 104 bps INFO @ Sat, 03 Apr 2021 09:33:56: #2 alternative fragment length(s) may be 104 bps INFO @ Sat, 03 Apr 2021 09:33:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05_model.r INFO @ Sat, 03 Apr 2021 09:33:56: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:33:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:34:01: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:34:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:34:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:34:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:34:08: 5000000 INFO @ Sat, 03 Apr 2021 09:34:12: 1000000 INFO @ Sat, 03 Apr 2021 09:34:14: 6000000 INFO @ Sat, 03 Apr 2021 09:34:16: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:34:19: 2000000 INFO @ Sat, 03 Apr 2021 09:34:21: 7000000 INFO @ Sat, 03 Apr 2021 09:34:25: 3000000 INFO @ Sat, 03 Apr 2021 09:34:26: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:26: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:26: #1 total tags in treatment: 7821718 INFO @ Sat, 03 Apr 2021 09:34:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:27: #1 tags after filtering in treatment: 7821605 INFO @ Sat, 03 Apr 2021 09:34:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:27: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:28: #2 number of paired peaks: 5636 INFO @ Sat, 03 Apr 2021 09:34:28: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:28: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:28: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:28: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:28: #2 predicted fragment length is 104 bps INFO @ Sat, 03 Apr 2021 09:34:28: #2 alternative fragment length(s) may be 104 bps INFO @ Sat, 03 Apr 2021 09:34:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10_model.r INFO @ Sat, 03 Apr 2021 09:34:28: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:28: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:34:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:34:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:34:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.05_summits.bed INFO @ Sat, 03 Apr 2021 09:34:28: Done! pass1 - making usageList (247 chroms): 4 millis pass2 - checking and writing primary data (19047 records, 4 fields): 25 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:34:32: 4000000 INFO @ Sat, 03 Apr 2021 09:34:38: 5000000 INFO @ Sat, 03 Apr 2021 09:34:44: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:34:46: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:34:50: 7000000 INFO @ Sat, 03 Apr 2021 09:34:55: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:55: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:55: #1 total tags in treatment: 7821718 INFO @ Sat, 03 Apr 2021 09:34:55: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:56: #1 tags after filtering in treatment: 7821605 INFO @ Sat, 03 Apr 2021 09:34:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:56: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:56: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:34:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:34:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.10_summits.bed INFO @ Sat, 03 Apr 2021 09:34:56: Done! INFO @ Sat, 03 Apr 2021 09:34:57: #2 number of paired peaks: 5636 INFO @ Sat, 03 Apr 2021 09:34:57: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:57: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:57: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:57: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:57: #2 predicted fragment length is 104 bps INFO @ Sat, 03 Apr 2021 09:34:57: #2 alternative fragment length(s) may be 104 bps INFO @ Sat, 03 Apr 2021 09:34:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20_model.r INFO @ Sat, 03 Apr 2021 09:34:57: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:57: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (154 chroms): 2 millis pass2 - checking and writing primary data (13351 records, 4 fields): 18 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:35:17: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:35:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:35:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:35:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282932/SRX7282932.20_summits.bed INFO @ Sat, 03 Apr 2021 09:35:28: Done! pass1 - making usageList (73 chroms): 2 millis pass2 - checking and writing primary data (7151 records, 4 fields): 10 millis CompletedMACS2peakCalling