Job ID = 12266827 SRX = SRX7282929 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:48 19888016 reads; of these: 19888016 (100.00%) were unpaired; of these: 2633522 (13.24%) aligned 0 times 15856426 (79.73%) aligned exactly 1 time 1398068 (7.03%) aligned >1 times 86.76% overall alignment rate Time searching: 00:03:48 Overall time: 00:03:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8362905 / 17254494 = 0.4847 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:32:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:32:58: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:32:58: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:33:04: 1000000 INFO @ Sat, 03 Apr 2021 09:33:11: 2000000 INFO @ Sat, 03 Apr 2021 09:33:18: 3000000 INFO @ Sat, 03 Apr 2021 09:33:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:33:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:33:28: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:33:28: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:33:32: 5000000 INFO @ Sat, 03 Apr 2021 09:33:35: 1000000 INFO @ Sat, 03 Apr 2021 09:33:38: 6000000 INFO @ Sat, 03 Apr 2021 09:33:41: 2000000 INFO @ Sat, 03 Apr 2021 09:33:46: 7000000 INFO @ Sat, 03 Apr 2021 09:33:48: 3000000 INFO @ Sat, 03 Apr 2021 09:33:54: 8000000 INFO @ Sat, 03 Apr 2021 09:33:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:33:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:33:58: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:33:58: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:34:00: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:00: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:00: #1 total tags in treatment: 8891589 INFO @ Sat, 03 Apr 2021 09:34:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:01: #1 tags after filtering in treatment: 8891501 INFO @ Sat, 03 Apr 2021 09:34:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:01: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:01: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:01: 5000000 INFO @ Sat, 03 Apr 2021 09:34:02: #2 number of paired peaks: 3367 INFO @ Sat, 03 Apr 2021 09:34:02: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:02: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:02: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:02: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:02: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:34:02: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:34:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05_model.r INFO @ Sat, 03 Apr 2021 09:34:02: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:34:04: 1000000 INFO @ Sat, 03 Apr 2021 09:34:08: 6000000 INFO @ Sat, 03 Apr 2021 09:34:11: 2000000 INFO @ Sat, 03 Apr 2021 09:34:14: 7000000 INFO @ Sat, 03 Apr 2021 09:34:17: 3000000 INFO @ Sat, 03 Apr 2021 09:34:21: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:34:22: 8000000 INFO @ Sat, 03 Apr 2021 09:34:24: 4000000 INFO @ Sat, 03 Apr 2021 09:34:28: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:28: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:28: #1 total tags in treatment: 8891589 INFO @ Sat, 03 Apr 2021 09:34:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:29: #1 tags after filtering in treatment: 8891501 INFO @ Sat, 03 Apr 2021 09:34:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:29: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:30: #2 number of paired peaks: 3367 INFO @ Sat, 03 Apr 2021 09:34:30: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:30: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:30: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:30: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:30: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:34:30: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:34:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10_model.r INFO @ Sat, 03 Apr 2021 09:34:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:34:30: 5000000 INFO @ Sat, 03 Apr 2021 09:34:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:34:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:34:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.05_summits.bed INFO @ Sat, 03 Apr 2021 09:34:32: Done! pass1 - making usageList (159 chroms): 4 millis pass2 - checking and writing primary data (21405 records, 4 fields): 33 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:34:36: 6000000 INFO @ Sat, 03 Apr 2021 09:34:42: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:34:49: 8000000 INFO @ Sat, 03 Apr 2021 09:34:49: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:34:55: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:55: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:55: #1 total tags in treatment: 8891589 INFO @ Sat, 03 Apr 2021 09:34:55: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:55: #1 tags after filtering in treatment: 8891501 INFO @ Sat, 03 Apr 2021 09:34:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:55: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:55: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:56: #2 number of paired peaks: 3367 INFO @ Sat, 03 Apr 2021 09:34:56: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:56: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:56: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:56: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:56: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:34:56: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:34:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20_model.r INFO @ Sat, 03 Apr 2021 09:34:56: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:35:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:35:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:35:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.10_summits.bed INFO @ Sat, 03 Apr 2021 09:35:01: Done! pass1 - making usageList (107 chroms): 3 millis pass2 - checking and writing primary data (13584 records, 4 fields): 22 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:35:16: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:35:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:35:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:35:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282929/SRX7282929.20_summits.bed INFO @ Sat, 03 Apr 2021 09:35:27: Done! pass1 - making usageList (62 chroms): 2 millis pass2 - checking and writing primary data (6103 records, 4 fields): 12 millis CompletedMACS2peakCalling