Job ID = 12266828 SRX = SRX7282924 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 23162677 reads; of these: 23162677 (100.00%) were unpaired; of these: 3565677 (15.39%) aligned 0 times 18048047 (77.92%) aligned exactly 1 time 1548953 (6.69%) aligned >1 times 84.61% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12057551 / 19597000 = 0.6153 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:33:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:33:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:33:49: 1000000 INFO @ Sat, 03 Apr 2021 09:33:55: 2000000 INFO @ Sat, 03 Apr 2021 09:34:01: 3000000 INFO @ Sat, 03 Apr 2021 09:34:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:34:12: 5000000 INFO @ Sat, 03 Apr 2021 09:34:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:34:13: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:34:13: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:34:18: 6000000 INFO @ Sat, 03 Apr 2021 09:34:19: 1000000 INFO @ Sat, 03 Apr 2021 09:34:24: 7000000 INFO @ Sat, 03 Apr 2021 09:34:25: 2000000 INFO @ Sat, 03 Apr 2021 09:34:28: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:28: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:28: #1 total tags in treatment: 7539449 INFO @ Sat, 03 Apr 2021 09:34:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:34:28: #1 tags after filtering in treatment: 7539288 INFO @ Sat, 03 Apr 2021 09:34:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:28: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:34:29: #2 number of paired peaks: 6617 INFO @ Sat, 03 Apr 2021 09:34:29: start model_add_line... INFO @ Sat, 03 Apr 2021 09:34:29: start X-correlation... INFO @ Sat, 03 Apr 2021 09:34:29: end of X-cor INFO @ Sat, 03 Apr 2021 09:34:29: #2 finished! INFO @ Sat, 03 Apr 2021 09:34:29: #2 predicted fragment length is 105 bps INFO @ Sat, 03 Apr 2021 09:34:29: #2 alternative fragment length(s) may be 105 bps INFO @ Sat, 03 Apr 2021 09:34:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05_model.r INFO @ Sat, 03 Apr 2021 09:34:29: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:34:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:34:31: 3000000 INFO @ Sat, 03 Apr 2021 09:34:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:34:42: 5000000 INFO @ Sat, 03 Apr 2021 09:34:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:34:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:34:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:34:47: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:34:48: 6000000 INFO @ Sat, 03 Apr 2021 09:34:50: 1000000 INFO @ Sat, 03 Apr 2021 09:34:55: 7000000 INFO @ Sat, 03 Apr 2021 09:34:56: 2000000 INFO @ Sat, 03 Apr 2021 09:34:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:34:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:34:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.05_summits.bed INFO @ Sat, 03 Apr 2021 09:34:58: Done! INFO @ Sat, 03 Apr 2021 09:34:59: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:34:59: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:34:59: #1 total tags in treatment: 7539449 INFO @ Sat, 03 Apr 2021 09:34:59: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:34:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (202 chroms): 4 millis pass2 - checking and writing primary data (21121 records, 4 fields): 32 millis INFO @ Sat, 03 Apr 2021 09:34:59: #1 tags after filtering in treatment: 7539288 INFO @ Sat, 03 Apr 2021 09:34:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:34:59: #1 finished! INFO @ Sat, 03 Apr 2021 09:34:59: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:34:59: #2 looking for paired plus/minus strand peaks... CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:35:00: #2 number of paired peaks: 6617 INFO @ Sat, 03 Apr 2021 09:35:00: start model_add_line... INFO @ Sat, 03 Apr 2021 09:35:00: start X-correlation... INFO @ Sat, 03 Apr 2021 09:35:00: end of X-cor INFO @ Sat, 03 Apr 2021 09:35:00: #2 finished! INFO @ Sat, 03 Apr 2021 09:35:00: #2 predicted fragment length is 105 bps INFO @ Sat, 03 Apr 2021 09:35:00: #2 alternative fragment length(s) may be 105 bps INFO @ Sat, 03 Apr 2021 09:35:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10_model.r INFO @ Sat, 03 Apr 2021 09:35:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:35:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:35:02: 3000000 INFO @ Sat, 03 Apr 2021 09:35:08: 4000000 INFO @ Sat, 03 Apr 2021 09:35:14: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:35:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:35:20: 6000000 INFO @ Sat, 03 Apr 2021 09:35:26: 7000000 INFO @ Sat, 03 Apr 2021 09:35:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:35:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:35:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.10_summits.bed INFO @ Sat, 03 Apr 2021 09:35:29: Done! pass1 - making usageList (133 chroms): 3 millis pass2 - checking and writing primary data (14963 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:35:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:35:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:35:29: #1 total tags in treatment: 7539449 INFO @ Sat, 03 Apr 2021 09:35:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:35:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:35:30: #1 tags after filtering in treatment: 7539288 INFO @ Sat, 03 Apr 2021 09:35:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:35:30: #1 finished! INFO @ Sat, 03 Apr 2021 09:35:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:35:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:35:31: #2 number of paired peaks: 6617 INFO @ Sat, 03 Apr 2021 09:35:31: start model_add_line... INFO @ Sat, 03 Apr 2021 09:35:31: start X-correlation... INFO @ Sat, 03 Apr 2021 09:35:31: end of X-cor INFO @ Sat, 03 Apr 2021 09:35:31: #2 finished! INFO @ Sat, 03 Apr 2021 09:35:31: #2 predicted fragment length is 105 bps INFO @ Sat, 03 Apr 2021 09:35:31: #2 alternative fragment length(s) may be 105 bps INFO @ Sat, 03 Apr 2021 09:35:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20_model.r INFO @ Sat, 03 Apr 2021 09:35:31: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:35:31: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:35:50: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:36:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:36:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:36:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282924/SRX7282924.20_summits.bed INFO @ Sat, 03 Apr 2021 09:36:00: Done! pass1 - making usageList (91 chroms): 2 millis pass2 - checking and writing primary data (8274 records, 4 fields): 13 millis CompletedMACS2peakCalling