Job ID = 12266783 SRX = SRX7282923 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:02 9821907 reads; of these: 9821907 (100.00%) were unpaired; of these: 1512666 (15.40%) aligned 0 times 7242339 (73.74%) aligned exactly 1 time 1066902 (10.86%) aligned >1 times 84.60% overall alignment rate Time searching: 00:02:02 Overall time: 00:02:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 2387581 / 8309241 = 0.2873 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:26:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:26:01: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:26:01: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:26:07: 1000000 INFO @ Sat, 03 Apr 2021 09:26:13: 2000000 INFO @ Sat, 03 Apr 2021 09:26:20: 3000000 INFO @ Sat, 03 Apr 2021 09:26:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:26:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:26:31: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:26:31: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:26:36: 5000000 INFO @ Sat, 03 Apr 2021 09:26:37: 1000000 INFO @ Sat, 03 Apr 2021 09:26:44: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:26:44: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:26:44: #1 total tags in treatment: 5921660 INFO @ Sat, 03 Apr 2021 09:26:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:26:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:26:44: 2000000 INFO @ Sat, 03 Apr 2021 09:26:45: #1 tags after filtering in treatment: 5921581 INFO @ Sat, 03 Apr 2021 09:26:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:26:45: #1 finished! INFO @ Sat, 03 Apr 2021 09:26:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:26:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:26:45: #2 number of paired peaks: 2843 INFO @ Sat, 03 Apr 2021 09:26:45: start model_add_line... INFO @ Sat, 03 Apr 2021 09:26:45: start X-correlation... INFO @ Sat, 03 Apr 2021 09:26:45: end of X-cor INFO @ Sat, 03 Apr 2021 09:26:45: #2 finished! INFO @ Sat, 03 Apr 2021 09:26:45: #2 predicted fragment length is 101 bps INFO @ Sat, 03 Apr 2021 09:26:45: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 03 Apr 2021 09:26:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05_model.r INFO @ Sat, 03 Apr 2021 09:26:45: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:26:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:26:50: 3000000 INFO @ Sat, 03 Apr 2021 09:26:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:26:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:27:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:27:01: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:27:01: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:27:05: 5000000 INFO @ Sat, 03 Apr 2021 09:27:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:27:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:27:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.05_summits.bed INFO @ Sat, 03 Apr 2021 09:27:07: Done! pass1 - making usageList (230 chroms): 3 millis pass2 - checking and writing primary data (11226 records, 4 fields): 26 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:27:09: 1000000 INFO @ Sat, 03 Apr 2021 09:27:12: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:27:12: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:27:12: #1 total tags in treatment: 5921660 INFO @ Sat, 03 Apr 2021 09:27:12: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:27:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:27:13: #1 tags after filtering in treatment: 5921581 INFO @ Sat, 03 Apr 2021 09:27:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:27:13: #1 finished! INFO @ Sat, 03 Apr 2021 09:27:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:27:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:27:13: #2 number of paired peaks: 2843 INFO @ Sat, 03 Apr 2021 09:27:13: start model_add_line... INFO @ Sat, 03 Apr 2021 09:27:13: start X-correlation... INFO @ Sat, 03 Apr 2021 09:27:13: end of X-cor INFO @ Sat, 03 Apr 2021 09:27:13: #2 finished! INFO @ Sat, 03 Apr 2021 09:27:13: #2 predicted fragment length is 101 bps INFO @ Sat, 03 Apr 2021 09:27:13: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 03 Apr 2021 09:27:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10_model.r INFO @ Sat, 03 Apr 2021 09:27:13: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:27:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:27:16: 2000000 INFO @ Sat, 03 Apr 2021 09:27:23: 3000000 INFO @ Sat, 03 Apr 2021 09:27:27: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:27:30: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:27:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:27:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:27:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.10_summits.bed INFO @ Sat, 03 Apr 2021 09:27:34: Done! pass1 - making usageList (134 chroms): 2 millis pass2 - checking and writing primary data (6626 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:27:37: 5000000 INFO @ Sat, 03 Apr 2021 09:27:43: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:27:43: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:27:43: #1 total tags in treatment: 5921660 INFO @ Sat, 03 Apr 2021 09:27:43: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:27:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:27:43: #1 tags after filtering in treatment: 5921581 INFO @ Sat, 03 Apr 2021 09:27:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:27:43: #1 finished! INFO @ Sat, 03 Apr 2021 09:27:43: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:27:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:27:44: #2 number of paired peaks: 2843 INFO @ Sat, 03 Apr 2021 09:27:44: start model_add_line... INFO @ Sat, 03 Apr 2021 09:27:44: start X-correlation... INFO @ Sat, 03 Apr 2021 09:27:44: end of X-cor INFO @ Sat, 03 Apr 2021 09:27:44: #2 finished! INFO @ Sat, 03 Apr 2021 09:27:44: #2 predicted fragment length is 101 bps INFO @ Sat, 03 Apr 2021 09:27:44: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 03 Apr 2021 09:27:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20_model.r INFO @ Sat, 03 Apr 2021 09:27:44: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:27:44: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:27:57: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:28:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:28:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:28:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282923/SRX7282923.20_summits.bed INFO @ Sat, 03 Apr 2021 09:28:04: Done! pass1 - making usageList (73 chroms): 2 millis pass2 - checking and writing primary data (2639 records, 4 fields): 8 millis CompletedMACS2peakCalling