Job ID = 12266377 SRX = SRX7282765 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:11 386799 reads; of these: 386799 (100.00%) were unpaired; of these: 47181 (12.20%) aligned 0 times 193198 (49.95%) aligned exactly 1 time 146420 (37.85%) aligned >1 times 87.80% overall alignment rate Time searching: 00:00:11 Overall time: 00:00:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 180730 / 339618 = 0.5322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:57:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:57:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:57:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:57:21: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:57:21: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:57:21: #1 total tags in treatment: 158888 INFO @ Sat, 03 Apr 2021 08:57:21: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:57:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:57:22: #1 tags after filtering in treatment: 158274 INFO @ Sat, 03 Apr 2021 08:57:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:57:22: #1 finished! INFO @ Sat, 03 Apr 2021 08:57:22: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:57:22: #2 number of paired peaks: 3235 INFO @ Sat, 03 Apr 2021 08:57:22: start model_add_line... INFO @ Sat, 03 Apr 2021 08:57:22: start X-correlation... INFO @ Sat, 03 Apr 2021 08:57:22: end of X-cor INFO @ Sat, 03 Apr 2021 08:57:22: #2 finished! INFO @ Sat, 03 Apr 2021 08:57:22: #2 predicted fragment length is 291 bps INFO @ Sat, 03 Apr 2021 08:57:22: #2 alternative fragment length(s) may be 10,38,58,115,148,221,253,265,291,508 bps INFO @ Sat, 03 Apr 2021 08:57:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05_model.r INFO @ Sat, 03 Apr 2021 08:57:22: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:57:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:57:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:57:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:57:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:57:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.05_summits.bed INFO @ Sat, 03 Apr 2021 08:57:23: Done! pass1 - making usageList (100 chroms): 1 millis pass2 - checking and writing primary data (126 records, 4 fields): 7 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:57:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:57:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:57:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:57:51: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:57:51: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:57:51: #1 total tags in treatment: 158888 INFO @ Sat, 03 Apr 2021 08:57:51: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:57:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:57:52: #1 tags after filtering in treatment: 158274 INFO @ Sat, 03 Apr 2021 08:57:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:57:52: #1 finished! INFO @ Sat, 03 Apr 2021 08:57:52: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:57:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:57:52: #2 number of paired peaks: 3235 INFO @ Sat, 03 Apr 2021 08:57:52: start model_add_line... INFO @ Sat, 03 Apr 2021 08:57:52: start X-correlation... INFO @ Sat, 03 Apr 2021 08:57:52: end of X-cor INFO @ Sat, 03 Apr 2021 08:57:52: #2 finished! INFO @ Sat, 03 Apr 2021 08:57:52: #2 predicted fragment length is 291 bps INFO @ Sat, 03 Apr 2021 08:57:52: #2 alternative fragment length(s) may be 10,38,58,115,148,221,253,265,291,508 bps INFO @ Sat, 03 Apr 2021 08:57:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10_model.r INFO @ Sat, 03 Apr 2021 08:57:52: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:57:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:57:53: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:57:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:57:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:57:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.10_summits.bed INFO @ Sat, 03 Apr 2021 08:57:53: Done! pass1 - making usageList (64 chroms): 1 millis pass2 - checking and writing primary data (82 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:58:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:58:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:58:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:58:21: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:58:21: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:58:21: #1 total tags in treatment: 158888 INFO @ Sat, 03 Apr 2021 08:58:21: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:58:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:58:22: #1 tags after filtering in treatment: 158274 INFO @ Sat, 03 Apr 2021 08:58:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:58:22: #1 finished! INFO @ Sat, 03 Apr 2021 08:58:22: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:58:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:58:22: #2 number of paired peaks: 3235 INFO @ Sat, 03 Apr 2021 08:58:22: start model_add_line... INFO @ Sat, 03 Apr 2021 08:58:22: start X-correlation... INFO @ Sat, 03 Apr 2021 08:58:22: end of X-cor INFO @ Sat, 03 Apr 2021 08:58:22: #2 finished! INFO @ Sat, 03 Apr 2021 08:58:22: #2 predicted fragment length is 291 bps INFO @ Sat, 03 Apr 2021 08:58:22: #2 alternative fragment length(s) may be 10,38,58,115,148,221,253,265,291,508 bps INFO @ Sat, 03 Apr 2021 08:58:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20_model.r INFO @ Sat, 03 Apr 2021 08:58:22: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:58:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:58:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:58:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:58:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:58:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282765/SRX7282765.20_summits.bed INFO @ Sat, 03 Apr 2021 08:58:23: Done! pass1 - making usageList (37 chroms): 1 millis pass2 - checking and writing primary data (50 records, 4 fields): 3 millis CompletedMACS2peakCalling