Job ID = 12266226 SRX = SRX7282676 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 17251 reads; of these: 17251 (100.00%) were unpaired; of these: 7208 (41.78%) aligned 0 times 8635 (50.06%) aligned exactly 1 time 1408 (8.16%) aligned >1 times 58.22% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 4728 / 10043 = 0.4708 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:52:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:52:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:52:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:52:30: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:52:30: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:52:30: #1 total tags in treatment: 5315 INFO @ Sat, 03 Apr 2021 08:52:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:52:30: #1 tags after filtering in treatment: 5165 INFO @ Sat, 03 Apr 2021 08:52:30: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 03 Apr 2021 08:52:30: #1 finished! INFO @ Sat, 03 Apr 2021 08:52:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:52:30: #2 number of paired peaks: 157 WARNING @ Sat, 03 Apr 2021 08:52:30: Fewer paired peaks (157) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 157 pairs to build model! INFO @ Sat, 03 Apr 2021 08:52:30: start model_add_line... INFO @ Sat, 03 Apr 2021 08:52:30: start X-correlation... INFO @ Sat, 03 Apr 2021 08:52:30: end of X-cor INFO @ Sat, 03 Apr 2021 08:52:30: #2 finished! INFO @ Sat, 03 Apr 2021 08:52:30: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:52:30: #2 alternative fragment length(s) may be 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 bps INFO @ Sat, 03 Apr 2021 08:52:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05_model.r WARNING @ Sat, 03 Apr 2021 08:52:30: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:52:30: #2 You may need to consider one of the other alternative d(s): 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 WARNING @ Sat, 03 Apr 2021 08:52:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:52:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:52:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:52:30: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:52:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:52:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:52:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.05_summits.bed INFO @ Sat, 03 Apr 2021 08:52:30: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:53:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:53:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:53:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:53:00: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:53:00: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:53:00: #1 total tags in treatment: 5315 INFO @ Sat, 03 Apr 2021 08:53:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:53:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:53:00: #1 tags after filtering in treatment: 5165 INFO @ Sat, 03 Apr 2021 08:53:00: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 03 Apr 2021 08:53:00: #1 finished! INFO @ Sat, 03 Apr 2021 08:53:00: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:53:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:53:00: #2 number of paired peaks: 157 WARNING @ Sat, 03 Apr 2021 08:53:00: Fewer paired peaks (157) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 157 pairs to build model! INFO @ Sat, 03 Apr 2021 08:53:00: start model_add_line... INFO @ Sat, 03 Apr 2021 08:53:00: start X-correlation... INFO @ Sat, 03 Apr 2021 08:53:00: end of X-cor INFO @ Sat, 03 Apr 2021 08:53:00: #2 finished! INFO @ Sat, 03 Apr 2021 08:53:00: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:53:00: #2 alternative fragment length(s) may be 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 bps INFO @ Sat, 03 Apr 2021 08:53:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10_model.r WARNING @ Sat, 03 Apr 2021 08:53:00: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:53:00: #2 You may need to consider one of the other alternative d(s): 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 WARNING @ Sat, 03 Apr 2021 08:53:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:53:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:53:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:53:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:53:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:53:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:53:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.10_summits.bed INFO @ Sat, 03 Apr 2021 08:53:00: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:53:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:53:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:53:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:53:30: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:53:30: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:53:30: #1 total tags in treatment: 5315 INFO @ Sat, 03 Apr 2021 08:53:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:53:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:53:30: #1 tags after filtering in treatment: 5165 INFO @ Sat, 03 Apr 2021 08:53:30: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 03 Apr 2021 08:53:30: #1 finished! INFO @ Sat, 03 Apr 2021 08:53:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:53:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:53:30: #2 number of paired peaks: 157 WARNING @ Sat, 03 Apr 2021 08:53:30: Fewer paired peaks (157) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 157 pairs to build model! INFO @ Sat, 03 Apr 2021 08:53:30: start model_add_line... INFO @ Sat, 03 Apr 2021 08:53:30: start X-correlation... INFO @ Sat, 03 Apr 2021 08:53:30: end of X-cor INFO @ Sat, 03 Apr 2021 08:53:30: #2 finished! INFO @ Sat, 03 Apr 2021 08:53:30: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:53:30: #2 alternative fragment length(s) may be 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 bps INFO @ Sat, 03 Apr 2021 08:53:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20_model.r WARNING @ Sat, 03 Apr 2021 08:53:30: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:53:30: #2 You may need to consider one of the other alternative d(s): 9,32,62,83,113,134,189,222,243,266,293,324,368,397,424,441,480,510,530,581 WARNING @ Sat, 03 Apr 2021 08:53:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:53:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:53:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:53:30: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:53:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:53:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:53:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7282676/SRX7282676.20_summits.bed INFO @ Sat, 03 Apr 2021 08:53:30: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling