Job ID = 12266056 SRX = SRX7282591 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 17208 reads; of these: 17208 (100.00%) were unpaired; of these: 9094 (52.85%) aligned 0 times 7171 (41.67%) aligned exactly 1 time 943 (5.48%) aligned >1 times 47.15% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 4078 / 8114 = 0.5026 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:45:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:45:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:45:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:45:49: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:45:49: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:45:49: #1 total tags in treatment: 4036 INFO @ Sat, 03 Apr 2021 08:45:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:45:49: #1 tags after filtering in treatment: 3958 INFO @ Sat, 03 Apr 2021 08:45:49: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 08:45:49: #1 finished! INFO @ Sat, 03 Apr 2021 08:45:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:45:49: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:45:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:45:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:46:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:19: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:19: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:19: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:46:19: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:46:19: #1 total tags in treatment: 4036 INFO @ Sat, 03 Apr 2021 08:46:19: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:46:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:46:19: #1 tags after filtering in treatment: 3958 INFO @ Sat, 03 Apr 2021 08:46:19: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 08:46:19: #1 finished! INFO @ Sat, 03 Apr 2021 08:46:19: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:46:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:46:19: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:46:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:46:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:46:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:49: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:46:49: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:46:49: #1 total tags in treatment: 4036 INFO @ Sat, 03 Apr 2021 08:46:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:46:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:46:49: #1 tags after filtering in treatment: 3958 INFO @ Sat, 03 Apr 2021 08:46:49: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 08:46:49: #1 finished! INFO @ Sat, 03 Apr 2021 08:46:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:46:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:46:49: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:46:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:46:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7282591/SRX7282591.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling