Job ID = 6626803 SRX = SRX7262571 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:25 55282961 reads; of these: 55282961 (100.00%) were unpaired; of these: 49520091 (89.58%) aligned 0 times 4454220 (8.06%) aligned exactly 1 time 1308650 (2.37%) aligned >1 times 10.42% overall alignment rate Time searching: 00:09:25 Overall time: 00:09:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 973659 / 5762870 = 0.1690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:01:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:01:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:01:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:01:42: 1000000 INFO @ Tue, 14 Jul 2020 09:01:48: 2000000 INFO @ Tue, 14 Jul 2020 09:01:54: 3000000 INFO @ Tue, 14 Jul 2020 09:02:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:06: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:06: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:06: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 09:02:06: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 09:02:06: #1 total tags in treatment: 4789211 INFO @ Tue, 14 Jul 2020 09:02:06: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:02:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:02:07: #1 tags after filtering in treatment: 4788910 INFO @ Tue, 14 Jul 2020 09:02:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:02:07: #1 finished! INFO @ Tue, 14 Jul 2020 09:02:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:02:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:02:08: #2 number of paired peaks: 7668 INFO @ Tue, 14 Jul 2020 09:02:08: start model_add_line... INFO @ Tue, 14 Jul 2020 09:02:08: start X-correlation... INFO @ Tue, 14 Jul 2020 09:02:08: end of X-cor INFO @ Tue, 14 Jul 2020 09:02:08: #2 finished! INFO @ Tue, 14 Jul 2020 09:02:08: #2 predicted fragment length is 141 bps INFO @ Tue, 14 Jul 2020 09:02:08: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 14 Jul 2020 09:02:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05_model.r WARNING @ Tue, 14 Jul 2020 09:02:08: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 09:02:08: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Tue, 14 Jul 2020 09:02:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 09:02:08: #3 Call peaks... INFO @ Tue, 14 Jul 2020 09:02:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 09:02:12: 1000000 INFO @ Tue, 14 Jul 2020 09:02:19: 2000000 INFO @ Tue, 14 Jul 2020 09:02:24: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 09:02:25: 3000000 INFO @ Tue, 14 Jul 2020 09:02:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05_peaks.xls INFO @ Tue, 14 Jul 2020 09:02:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 09:02:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.05_summits.bed INFO @ Tue, 14 Jul 2020 09:02:31: Done! pass1 - making usageList (183 chroms): 1 millis pass2 - checking and writing primary data (5833 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:02:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:02:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:02:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:02:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:02:38: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 09:02:38: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 09:02:38: #1 total tags in treatment: 4789211 INFO @ Tue, 14 Jul 2020 09:02:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:02:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:02:38: #1 tags after filtering in treatment: 4788910 INFO @ Tue, 14 Jul 2020 09:02:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:02:38: #1 finished! INFO @ Tue, 14 Jul 2020 09:02:38: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:02:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:02:39: #2 number of paired peaks: 7668 INFO @ Tue, 14 Jul 2020 09:02:39: start model_add_line... INFO @ Tue, 14 Jul 2020 09:02:39: start X-correlation... INFO @ Tue, 14 Jul 2020 09:02:39: end of X-cor INFO @ Tue, 14 Jul 2020 09:02:39: #2 finished! INFO @ Tue, 14 Jul 2020 09:02:39: #2 predicted fragment length is 141 bps INFO @ Tue, 14 Jul 2020 09:02:39: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 14 Jul 2020 09:02:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10_model.r WARNING @ Tue, 14 Jul 2020 09:02:39: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 09:02:39: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Tue, 14 Jul 2020 09:02:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 09:02:39: #3 Call peaks... INFO @ Tue, 14 Jul 2020 09:02:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 09:02:42: 1000000 INFO @ Tue, 14 Jul 2020 09:02:49: 2000000 INFO @ Tue, 14 Jul 2020 09:02:54: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 09:02:55: 3000000 INFO @ Tue, 14 Jul 2020 09:03:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10_peaks.xls INFO @ Tue, 14 Jul 2020 09:03:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 09:03:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.10_summits.bed INFO @ Tue, 14 Jul 2020 09:03:01: Done! pass1 - making usageList (142 chroms): 2 millis pass2 - checking and writing primary data (3853 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:03:02: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:03:07: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 09:03:07: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 09:03:07: #1 total tags in treatment: 4789211 INFO @ Tue, 14 Jul 2020 09:03:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:03:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:03:07: #1 tags after filtering in treatment: 4788910 INFO @ Tue, 14 Jul 2020 09:03:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:03:07: #1 finished! INFO @ Tue, 14 Jul 2020 09:03:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:03:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:03:08: #2 number of paired peaks: 7668 INFO @ Tue, 14 Jul 2020 09:03:08: start model_add_line... INFO @ Tue, 14 Jul 2020 09:03:08: start X-correlation... INFO @ Tue, 14 Jul 2020 09:03:08: end of X-cor INFO @ Tue, 14 Jul 2020 09:03:08: #2 finished! INFO @ Tue, 14 Jul 2020 09:03:08: #2 predicted fragment length is 141 bps INFO @ Tue, 14 Jul 2020 09:03:08: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 14 Jul 2020 09:03:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20_model.r WARNING @ Tue, 14 Jul 2020 09:03:08: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 09:03:08: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Tue, 14 Jul 2020 09:03:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 09:03:08: #3 Call peaks... INFO @ Tue, 14 Jul 2020 09:03:08: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:03:24: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 09:03:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20_peaks.xls INFO @ Tue, 14 Jul 2020 09:03:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 09:03:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262571/SRX7262571.20_summits.bed INFO @ Tue, 14 Jul 2020 09:03:31: Done! pass1 - making usageList (101 chroms): 1 millis pass2 - checking and writing primary data (2125 records, 4 fields): 8 millis CompletedMACS2peakCalling