Job ID = 6626753 SRX = SRX7262541 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:08 29686037 reads; of these: 29686037 (100.00%) were unpaired; of these: 21341629 (71.89%) aligned 0 times 6876210 (23.16%) aligned exactly 1 time 1468198 (4.95%) aligned >1 times 28.11% overall alignment rate Time searching: 00:06:08 Overall time: 00:06:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1895995 / 8344408 = 0.2272 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:45:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:45:20: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:45:20: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:45:29: 1000000 INFO @ Tue, 14 Jul 2020 08:45:39: 2000000 INFO @ Tue, 14 Jul 2020 08:45:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:45:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:45:50: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:45:50: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:45:52: 4000000 INFO @ Tue, 14 Jul 2020 08:45:59: 1000000 INFO @ Tue, 14 Jul 2020 08:46:00: 5000000 INFO @ Tue, 14 Jul 2020 08:46:08: 6000000 INFO @ Tue, 14 Jul 2020 08:46:08: 2000000 INFO @ Tue, 14 Jul 2020 08:46:11: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:46:11: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:46:11: #1 total tags in treatment: 6448413 INFO @ Tue, 14 Jul 2020 08:46:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:46:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:46:11: #1 tags after filtering in treatment: 6447753 INFO @ Tue, 14 Jul 2020 08:46:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:46:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:46:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:46:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:46:12: #2 number of paired peaks: 10146 INFO @ Tue, 14 Jul 2020 08:46:12: start model_add_line... INFO @ Tue, 14 Jul 2020 08:46:13: start X-correlation... INFO @ Tue, 14 Jul 2020 08:46:13: end of X-cor INFO @ Tue, 14 Jul 2020 08:46:13: #2 finished! INFO @ Tue, 14 Jul 2020 08:46:13: #2 predicted fragment length is 184 bps INFO @ Tue, 14 Jul 2020 08:46:13: #2 alternative fragment length(s) may be 184 bps INFO @ Tue, 14 Jul 2020 08:46:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05_model.r INFO @ Tue, 14 Jul 2020 08:46:13: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:46:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:46:16: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:46:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:46:20: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:46:20: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:46:24: 4000000 INFO @ Tue, 14 Jul 2020 08:46:29: 1000000 INFO @ Tue, 14 Jul 2020 08:46:33: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:46:33: 5000000 INFO @ Tue, 14 Jul 2020 08:46:38: 2000000 INFO @ Tue, 14 Jul 2020 08:46:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05_peaks.xls INFO @ Tue, 14 Jul 2020 08:46:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:46:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.05_summits.bed INFO @ Tue, 14 Jul 2020 08:46:42: Done! INFO @ Tue, 14 Jul 2020 08:46:42: 6000000 pass1 - making usageList (137 chroms): 1 millis pass2 - checking and writing primary data (7307 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:46:45: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:46:45: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:46:45: #1 total tags in treatment: 6448413 INFO @ Tue, 14 Jul 2020 08:46:45: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:46:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:46:46: #1 tags after filtering in treatment: 6447753 INFO @ Tue, 14 Jul 2020 08:46:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:46:46: #1 finished! INFO @ Tue, 14 Jul 2020 08:46:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:46:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:46:46: 3000000 INFO @ Tue, 14 Jul 2020 08:46:47: #2 number of paired peaks: 10146 INFO @ Tue, 14 Jul 2020 08:46:47: start model_add_line... INFO @ Tue, 14 Jul 2020 08:46:47: start X-correlation... INFO @ Tue, 14 Jul 2020 08:46:47: end of X-cor INFO @ Tue, 14 Jul 2020 08:46:47: #2 finished! INFO @ Tue, 14 Jul 2020 08:46:47: #2 predicted fragment length is 184 bps INFO @ Tue, 14 Jul 2020 08:46:47: #2 alternative fragment length(s) may be 184 bps INFO @ Tue, 14 Jul 2020 08:46:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10_model.r INFO @ Tue, 14 Jul 2020 08:46:47: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:46:47: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:46:54: 4000000 INFO @ Tue, 14 Jul 2020 08:47:02: 5000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:47:07: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:47:10: 6000000 INFO @ Tue, 14 Jul 2020 08:47:14: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:47:14: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:47:14: #1 total tags in treatment: 6448413 INFO @ Tue, 14 Jul 2020 08:47:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:47:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:47:14: #1 tags after filtering in treatment: 6447753 INFO @ Tue, 14 Jul 2020 08:47:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:47:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:47:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:47:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:47:15: #2 number of paired peaks: 10146 INFO @ Tue, 14 Jul 2020 08:47:15: start model_add_line... INFO @ Tue, 14 Jul 2020 08:47:15: start X-correlation... INFO @ Tue, 14 Jul 2020 08:47:15: end of X-cor INFO @ Tue, 14 Jul 2020 08:47:15: #2 finished! INFO @ Tue, 14 Jul 2020 08:47:15: #2 predicted fragment length is 184 bps INFO @ Tue, 14 Jul 2020 08:47:15: #2 alternative fragment length(s) may be 184 bps INFO @ Tue, 14 Jul 2020 08:47:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20_model.r INFO @ Tue, 14 Jul 2020 08:47:15: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:47:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:47:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10_peaks.xls INFO @ Tue, 14 Jul 2020 08:47:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:47:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.10_summits.bed INFO @ Tue, 14 Jul 2020 08:47:16: Done! pass1 - making usageList (118 chroms): 1 millis pass2 - checking and writing primary data (5883 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:47:35: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:47:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20_peaks.xls INFO @ Tue, 14 Jul 2020 08:47:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:47:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262541/SRX7262541.20_summits.bed INFO @ Tue, 14 Jul 2020 08:47:44: Done! pass1 - making usageList (104 chroms): 1 millis pass2 - checking and writing primary data (4392 records, 4 fields): 8 millis CompletedMACS2peakCalling