Job ID = 6626708 SRX = SRX7262526 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:46 30252534 reads; of these: 30252534 (100.00%) were unpaired; of these: 26423495 (87.34%) aligned 0 times 3172007 (10.49%) aligned exactly 1 time 657032 (2.17%) aligned >1 times 12.66% overall alignment rate Time searching: 00:04:46 Overall time: 00:04:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 481562 / 3829039 = 0.1258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:30:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:30:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:30:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:30:52: 1000000 INFO @ Tue, 14 Jul 2020 08:31:00: 2000000 INFO @ Tue, 14 Jul 2020 08:31:06: 3000000 INFO @ Tue, 14 Jul 2020 08:31:09: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:31:09: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:31:09: #1 total tags in treatment: 3347477 INFO @ Tue, 14 Jul 2020 08:31:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:31:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:31:09: #1 tags after filtering in treatment: 3346799 INFO @ Tue, 14 Jul 2020 08:31:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:31:09: #1 finished! INFO @ Tue, 14 Jul 2020 08:31:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:31:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:31:09: #2 number of paired peaks: 9062 INFO @ Tue, 14 Jul 2020 08:31:09: start model_add_line... INFO @ Tue, 14 Jul 2020 08:31:09: start X-correlation... INFO @ Tue, 14 Jul 2020 08:31:09: end of X-cor INFO @ Tue, 14 Jul 2020 08:31:09: #2 finished! INFO @ Tue, 14 Jul 2020 08:31:09: #2 predicted fragment length is 213 bps INFO @ Tue, 14 Jul 2020 08:31:09: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 14 Jul 2020 08:31:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05_model.r INFO @ Tue, 14 Jul 2020 08:31:09: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:31:09: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:31:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:31:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:31:20: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:31:22: 1000000 INFO @ Tue, 14 Jul 2020 08:31:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05_peaks.xls INFO @ Tue, 14 Jul 2020 08:31:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:31:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.05_summits.bed INFO @ Tue, 14 Jul 2020 08:31:24: Done! pass1 - making usageList (119 chroms): 1 millis pass2 - checking and writing primary data (4906 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:31:29: 2000000 INFO @ Tue, 14 Jul 2020 08:31:35: 3000000 INFO @ Tue, 14 Jul 2020 08:31:37: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:31:37: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:31:37: #1 total tags in treatment: 3347477 INFO @ Tue, 14 Jul 2020 08:31:37: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:31:37: #1 tags after filtering in treatment: 3346799 INFO @ Tue, 14 Jul 2020 08:31:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:31:37: #1 finished! INFO @ Tue, 14 Jul 2020 08:31:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:31:38: #2 number of paired peaks: 9062 INFO @ Tue, 14 Jul 2020 08:31:38: start model_add_line... INFO @ Tue, 14 Jul 2020 08:31:38: start X-correlation... INFO @ Tue, 14 Jul 2020 08:31:38: end of X-cor INFO @ Tue, 14 Jul 2020 08:31:38: #2 finished! INFO @ Tue, 14 Jul 2020 08:31:38: #2 predicted fragment length is 213 bps INFO @ Tue, 14 Jul 2020 08:31:38: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 14 Jul 2020 08:31:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10_model.r INFO @ Tue, 14 Jul 2020 08:31:38: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:31:38: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:31:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:31:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:31:48: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:31:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10_peaks.xls INFO @ Tue, 14 Jul 2020 08:31:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:31:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.10_summits.bed INFO @ Tue, 14 Jul 2020 08:31:53: Done! pass1 - making usageList (99 chroms): 2 millis pass2 - checking and writing primary data (3581 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:31:54: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:32:03: 2000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:32:11: 3000000 INFO @ Tue, 14 Jul 2020 08:32:14: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:32:14: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:32:14: #1 total tags in treatment: 3347477 INFO @ Tue, 14 Jul 2020 08:32:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:32:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:32:14: #1 tags after filtering in treatment: 3346799 INFO @ Tue, 14 Jul 2020 08:32:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:32:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:32:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:32:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:32:14: #2 number of paired peaks: 9062 INFO @ Tue, 14 Jul 2020 08:32:14: start model_add_line... INFO @ Tue, 14 Jul 2020 08:32:14: start X-correlation... INFO @ Tue, 14 Jul 2020 08:32:15: end of X-cor INFO @ Tue, 14 Jul 2020 08:32:15: #2 finished! INFO @ Tue, 14 Jul 2020 08:32:15: #2 predicted fragment length is 213 bps INFO @ Tue, 14 Jul 2020 08:32:15: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 14 Jul 2020 08:32:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20_model.r INFO @ Tue, 14 Jul 2020 08:32:15: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:32:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:32:26: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:32:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20_peaks.xls INFO @ Tue, 14 Jul 2020 08:32:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:32:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262526/SRX7262526.20_summits.bed INFO @ Tue, 14 Jul 2020 08:32:30: Done! pass1 - making usageList (82 chroms): 2 millis pass2 - checking and writing primary data (2323 records, 4 fields): 5 millis CompletedMACS2peakCalling