Job ID = 6626717 SRX = SRX7262525 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:11 34011650 reads; of these: 34011650 (100.00%) were unpaired; of these: 22039681 (64.80%) aligned 0 times 9915013 (29.15%) aligned exactly 1 time 2056956 (6.05%) aligned >1 times 35.20% overall alignment rate Time searching: 00:07:11 Overall time: 00:07:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2962097 / 11971969 = 0.2474 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:38:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:38:17: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:38:17: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:38:25: 1000000 INFO @ Tue, 14 Jul 2020 08:38:32: 2000000 INFO @ Tue, 14 Jul 2020 08:38:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:38:46: 4000000 INFO @ Tue, 14 Jul 2020 08:38:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:38:47: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:38:47: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:38:53: 5000000 INFO @ Tue, 14 Jul 2020 08:38:55: 1000000 INFO @ Tue, 14 Jul 2020 08:39:01: 6000000 INFO @ Tue, 14 Jul 2020 08:39:03: 2000000 INFO @ Tue, 14 Jul 2020 08:39:08: 7000000 INFO @ Tue, 14 Jul 2020 08:39:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:39:16: 8000000 INFO @ Tue, 14 Jul 2020 08:39:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:39:17: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:39:17: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:39:18: 4000000 INFO @ Tue, 14 Jul 2020 08:39:24: 9000000 INFO @ Tue, 14 Jul 2020 08:39:24: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:39:24: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:39:24: #1 total tags in treatment: 9009872 INFO @ Tue, 14 Jul 2020 08:39:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:39:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:39:25: #1 tags after filtering in treatment: 9009280 INFO @ Tue, 14 Jul 2020 08:39:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:39:25: #1 finished! INFO @ Tue, 14 Jul 2020 08:39:25: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:39:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:39:26: 1000000 INFO @ Tue, 14 Jul 2020 08:39:26: #2 number of paired peaks: 10167 INFO @ Tue, 14 Jul 2020 08:39:26: start model_add_line... INFO @ Tue, 14 Jul 2020 08:39:26: start X-correlation... INFO @ Tue, 14 Jul 2020 08:39:26: end of X-cor INFO @ Tue, 14 Jul 2020 08:39:26: #2 finished! INFO @ Tue, 14 Jul 2020 08:39:26: #2 predicted fragment length is 211 bps INFO @ Tue, 14 Jul 2020 08:39:26: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 14 Jul 2020 08:39:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05_model.r INFO @ Tue, 14 Jul 2020 08:39:26: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:39:26: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:39:27: 5000000 INFO @ Tue, 14 Jul 2020 08:39:34: 2000000 INFO @ Tue, 14 Jul 2020 08:39:35: 6000000 INFO @ Tue, 14 Jul 2020 08:39:42: 3000000 INFO @ Tue, 14 Jul 2020 08:39:43: 7000000 INFO @ Tue, 14 Jul 2020 08:39:51: 4000000 INFO @ Tue, 14 Jul 2020 08:39:52: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:39:59: 5000000 INFO @ Tue, 14 Jul 2020 08:40:00: 9000000 INFO @ Tue, 14 Jul 2020 08:40:00: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:40:00: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:40:00: #1 total tags in treatment: 9009872 INFO @ Tue, 14 Jul 2020 08:40:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:40:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:40:01: #1 tags after filtering in treatment: 9009280 INFO @ Tue, 14 Jul 2020 08:40:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:40:01: #1 finished! INFO @ Tue, 14 Jul 2020 08:40:01: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:40:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:40:02: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:40:02: #2 number of paired peaks: 10167 INFO @ Tue, 14 Jul 2020 08:40:02: start model_add_line... INFO @ Tue, 14 Jul 2020 08:40:02: start X-correlation... INFO @ Tue, 14 Jul 2020 08:40:02: end of X-cor INFO @ Tue, 14 Jul 2020 08:40:02: #2 finished! INFO @ Tue, 14 Jul 2020 08:40:02: #2 predicted fragment length is 211 bps INFO @ Tue, 14 Jul 2020 08:40:02: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 14 Jul 2020 08:40:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10_model.r INFO @ Tue, 14 Jul 2020 08:40:02: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:40:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:40:07: 6000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:40:14: 7000000 INFO @ Tue, 14 Jul 2020 08:40:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05_peaks.xls INFO @ Tue, 14 Jul 2020 08:40:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:40:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.05_summits.bed INFO @ Tue, 14 Jul 2020 08:40:16: Done! pass1 - making usageList (141 chroms): 2 millis pass2 - checking and writing primary data (7006 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:40:22: 8000000 INFO @ Tue, 14 Jul 2020 08:40:28: 9000000 INFO @ Tue, 14 Jul 2020 08:40:28: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:40:28: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:40:28: #1 total tags in treatment: 9009872 INFO @ Tue, 14 Jul 2020 08:40:28: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:40:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:40:28: #1 tags after filtering in treatment: 9009280 INFO @ Tue, 14 Jul 2020 08:40:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:40:28: #1 finished! INFO @ Tue, 14 Jul 2020 08:40:28: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:40:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:40:30: #2 number of paired peaks: 10167 INFO @ Tue, 14 Jul 2020 08:40:30: start model_add_line... INFO @ Tue, 14 Jul 2020 08:40:30: start X-correlation... INFO @ Tue, 14 Jul 2020 08:40:30: end of X-cor INFO @ Tue, 14 Jul 2020 08:40:30: #2 finished! INFO @ Tue, 14 Jul 2020 08:40:30: #2 predicted fragment length is 211 bps INFO @ Tue, 14 Jul 2020 08:40:30: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 14 Jul 2020 08:40:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20_model.r INFO @ Tue, 14 Jul 2020 08:40:30: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:40:30: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:40:35: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:40:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10_peaks.xls INFO @ Tue, 14 Jul 2020 08:40:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:40:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.10_summits.bed INFO @ Tue, 14 Jul 2020 08:40:48: Done! pass1 - making usageList (122 chroms): 1 millis pass2 - checking and writing primary data (5557 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:41:02: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:41:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20_peaks.xls INFO @ Tue, 14 Jul 2020 08:41:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:41:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7262525/SRX7262525.20_summits.bed INFO @ Tue, 14 Jul 2020 08:41:14: Done! pass1 - making usageList (104 chroms): 2 millis pass2 - checking and writing primary data (4004 records, 4 fields): 7 millis CompletedMACS2peakCalling