Job ID = 8069890 SRX = SRX7191471 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:46 16748185 reads; of these: 16748185 (100.00%) were unpaired; of these: 2729957 (16.30%) aligned 0 times 11496084 (68.64%) aligned exactly 1 time 2522144 (15.06%) aligned >1 times 83.70% overall alignment rate Time searching: 00:03:46 Overall time: 00:03:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2797652 / 14018228 = 0.1996 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:04:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:04:50: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:04:50: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:04:56: 1000000 INFO @ Sat, 08 Aug 2020 13:05:02: 2000000 INFO @ Sat, 08 Aug 2020 13:05:08: 3000000 INFO @ Sat, 08 Aug 2020 13:05:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:05:19: 5000000 INFO @ Sat, 08 Aug 2020 13:05:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:05:20: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:05:20: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:05:26: 6000000 INFO @ Sat, 08 Aug 2020 13:05:26: 1000000 INFO @ Sat, 08 Aug 2020 13:05:32: 7000000 INFO @ Sat, 08 Aug 2020 13:05:33: 2000000 INFO @ Sat, 08 Aug 2020 13:05:37: 8000000 INFO @ Sat, 08 Aug 2020 13:05:39: 3000000 INFO @ Sat, 08 Aug 2020 13:05:43: 9000000 INFO @ Sat, 08 Aug 2020 13:05:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:05:50: 10000000 INFO @ Sat, 08 Aug 2020 13:05:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:05:51: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:05:51: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:05:51: 5000000 INFO @ Sat, 08 Aug 2020 13:05:56: 11000000 INFO @ Sat, 08 Aug 2020 13:05:56: 1000000 INFO @ Sat, 08 Aug 2020 13:05:57: 6000000 INFO @ Sat, 08 Aug 2020 13:05:57: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 13:05:57: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 13:05:57: #1 total tags in treatment: 11220576 INFO @ Sat, 08 Aug 2020 13:05:57: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:05:57: #1 tags after filtering in treatment: 11220559 INFO @ Sat, 08 Aug 2020 13:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 13:05:57: #1 finished! INFO @ Sat, 08 Aug 2020 13:05:57: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:05:58: #2 number of paired peaks: 2765 INFO @ Sat, 08 Aug 2020 13:05:58: start model_add_line... INFO @ Sat, 08 Aug 2020 13:05:59: start X-correlation... INFO @ Sat, 08 Aug 2020 13:05:59: end of X-cor INFO @ Sat, 08 Aug 2020 13:05:59: #2 finished! INFO @ Sat, 08 Aug 2020 13:05:59: #2 predicted fragment length is 228 bps INFO @ Sat, 08 Aug 2020 13:05:59: #2 alternative fragment length(s) may be 228 bps INFO @ Sat, 08 Aug 2020 13:05:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05_model.r INFO @ Sat, 08 Aug 2020 13:05:59: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:05:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:06:01: 2000000 INFO @ Sat, 08 Aug 2020 13:06:03: 7000000 INFO @ Sat, 08 Aug 2020 13:06:07: 3000000 INFO @ Sat, 08 Aug 2020 13:06:09: 8000000 INFO @ Sat, 08 Aug 2020 13:06:12: 4000000 INFO @ Sat, 08 Aug 2020 13:06:15: 9000000 INFO @ Sat, 08 Aug 2020 13:06:17: 5000000 INFO @ Sat, 08 Aug 2020 13:06:22: 10000000 INFO @ Sat, 08 Aug 2020 13:06:23: 6000000 INFO @ Sat, 08 Aug 2020 13:06:27: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:06:28: 11000000 INFO @ Sat, 08 Aug 2020 13:06:28: 7000000 INFO @ Sat, 08 Aug 2020 13:06:29: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 13:06:29: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 13:06:29: #1 total tags in treatment: 11220576 INFO @ Sat, 08 Aug 2020 13:06:29: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:06:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:06:30: #1 tags after filtering in treatment: 11220559 INFO @ Sat, 08 Aug 2020 13:06:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 13:06:30: #1 finished! INFO @ Sat, 08 Aug 2020 13:06:30: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:06:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:06:31: #2 number of paired peaks: 2765 INFO @ Sat, 08 Aug 2020 13:06:31: start model_add_line... INFO @ Sat, 08 Aug 2020 13:06:31: start X-correlation... INFO @ Sat, 08 Aug 2020 13:06:31: end of X-cor INFO @ Sat, 08 Aug 2020 13:06:31: #2 finished! INFO @ Sat, 08 Aug 2020 13:06:31: #2 predicted fragment length is 228 bps INFO @ Sat, 08 Aug 2020 13:06:31: #2 alternative fragment length(s) may be 228 bps INFO @ Sat, 08 Aug 2020 13:06:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10_model.r INFO @ Sat, 08 Aug 2020 13:06:31: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:06:31: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:06:33: 8000000 INFO @ Sat, 08 Aug 2020 13:06:38: 9000000 INFO @ Sat, 08 Aug 2020 13:06:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05_peaks.xls INFO @ Sat, 08 Aug 2020 13:06:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:06:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.05_summits.bed INFO @ Sat, 08 Aug 2020 13:06:42: Done! pass1 - making usageList (395 chroms): 2 millis pass2 - checking and writing primary data (9765 records, 4 fields): 36 millis CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 13:06:43: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 13:06:49: 11000000 INFO @ Sat, 08 Aug 2020 13:06:50: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 13:06:50: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 13:06:50: #1 total tags in treatment: 11220576 INFO @ Sat, 08 Aug 2020 13:06:50: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:06:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:06:50: #1 tags after filtering in treatment: 11220559 INFO @ Sat, 08 Aug 2020 13:06:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 13:06:50: #1 finished! INFO @ Sat, 08 Aug 2020 13:06:50: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:06:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:06:51: #2 number of paired peaks: 2765 INFO @ Sat, 08 Aug 2020 13:06:51: start model_add_line... INFO @ Sat, 08 Aug 2020 13:06:51: start X-correlation... INFO @ Sat, 08 Aug 2020 13:06:51: end of X-cor INFO @ Sat, 08 Aug 2020 13:06:51: #2 finished! INFO @ Sat, 08 Aug 2020 13:06:51: #2 predicted fragment length is 228 bps INFO @ Sat, 08 Aug 2020 13:06:51: #2 alternative fragment length(s) may be 228 bps INFO @ Sat, 08 Aug 2020 13:06:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20_model.r INFO @ Sat, 08 Aug 2020 13:06:51: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:06:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:07:00: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 13:07:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10_peaks.xls INFO @ Sat, 08 Aug 2020 13:07:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:07:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.10_summits.bed INFO @ Sat, 08 Aug 2020 13:07:15: Done! pass1 - making usageList (302 chroms): 2 millis pass2 - checking and writing primary data (6855 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 13:07:21: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:07:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20_peaks.xls INFO @ Sat, 08 Aug 2020 13:07:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:07:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX7191471/SRX7191471.20_summits.bed INFO @ Sat, 08 Aug 2020 13:07:35: Done! pass1 - making usageList (238 chroms): 2 millis pass2 - checking and writing primary data (4378 records, 4 fields): 12 millis CompletedMACS2peakCalling