Job ID = 6459593 SRX = SRX7158310 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:32:31 prefetch.2.10.7: 1) Downloading 'SRR10467038'... 2020-06-21T13:32:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:44:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:44:50 prefetch.2.10.7: 1) 'SRR10467038' was downloaded successfully 2020-06-21T13:44:50 prefetch.2.10.7: 'SRR10467038' has 0 unresolved dependencies Read 72270441 spots for SRR10467038/SRR10467038.sra Written 72270441 spots for SRR10467038/SRR10467038.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:22 72270441 reads; of these: 72270441 (100.00%) were unpaired; of these: 5005837 (6.93%) aligned 0 times 39691162 (54.92%) aligned exactly 1 time 27573442 (38.15%) aligned >1 times 93.07% overall alignment rate Time searching: 00:37:22 Overall time: 00:37:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 19478683 / 67264604 = 0.2896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:45:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:45:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:45:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:46:03: 1000000 INFO @ Sun, 21 Jun 2020 23:46:10: 2000000 INFO @ Sun, 21 Jun 2020 23:46:16: 3000000 INFO @ Sun, 21 Jun 2020 23:46:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:46:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:46:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:46:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:46:28: 5000000 INFO @ Sun, 21 Jun 2020 23:46:35: 6000000 INFO @ Sun, 21 Jun 2020 23:46:35: 1000000 INFO @ Sun, 21 Jun 2020 23:46:41: 7000000 INFO @ Sun, 21 Jun 2020 23:46:42: 2000000 INFO @ Sun, 21 Jun 2020 23:46:48: 8000000 INFO @ Sun, 21 Jun 2020 23:46:49: 3000000 INFO @ Sun, 21 Jun 2020 23:46:55: 9000000 INFO @ Sun, 21 Jun 2020 23:46:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:46:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:46:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:46:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:47:02: 10000000 INFO @ Sun, 21 Jun 2020 23:47:02: 5000000 INFO @ Sun, 21 Jun 2020 23:47:04: 1000000 INFO @ Sun, 21 Jun 2020 23:47:08: 11000000 INFO @ Sun, 21 Jun 2020 23:47:09: 6000000 INFO @ Sun, 21 Jun 2020 23:47:11: 2000000 INFO @ Sun, 21 Jun 2020 23:47:15: 12000000 INFO @ Sun, 21 Jun 2020 23:47:16: 7000000 INFO @ Sun, 21 Jun 2020 23:47:18: 3000000 INFO @ Sun, 21 Jun 2020 23:47:22: 13000000 INFO @ Sun, 21 Jun 2020 23:47:22: 8000000 INFO @ Sun, 21 Jun 2020 23:47:26: 4000000 INFO @ Sun, 21 Jun 2020 23:47:29: 14000000 INFO @ Sun, 21 Jun 2020 23:47:30: 9000000 INFO @ Sun, 21 Jun 2020 23:47:33: 5000000 INFO @ Sun, 21 Jun 2020 23:47:36: 15000000 INFO @ Sun, 21 Jun 2020 23:47:37: 10000000 INFO @ Sun, 21 Jun 2020 23:47:40: 6000000 INFO @ Sun, 21 Jun 2020 23:47:43: 16000000 INFO @ Sun, 21 Jun 2020 23:47:45: 11000000 INFO @ Sun, 21 Jun 2020 23:47:47: 7000000 INFO @ Sun, 21 Jun 2020 23:47:50: 17000000 INFO @ Sun, 21 Jun 2020 23:47:52: 12000000 INFO @ Sun, 21 Jun 2020 23:47:54: 8000000 INFO @ Sun, 21 Jun 2020 23:47:57: 18000000 INFO @ Sun, 21 Jun 2020 23:47:59: 13000000 INFO @ Sun, 21 Jun 2020 23:48:02: 9000000 INFO @ Sun, 21 Jun 2020 23:48:04: 19000000 INFO @ Sun, 21 Jun 2020 23:48:06: 14000000 INFO @ Sun, 21 Jun 2020 23:48:09: 10000000 INFO @ Sun, 21 Jun 2020 23:48:11: 20000000 INFO @ Sun, 21 Jun 2020 23:48:13: 15000000 INFO @ Sun, 21 Jun 2020 23:48:16: 11000000 INFO @ Sun, 21 Jun 2020 23:48:17: 21000000 INFO @ Sun, 21 Jun 2020 23:48:20: 16000000 INFO @ Sun, 21 Jun 2020 23:48:23: 12000000 INFO @ Sun, 21 Jun 2020 23:48:24: 22000000 INFO @ Sun, 21 Jun 2020 23:48:27: 17000000 INFO @ Sun, 21 Jun 2020 23:48:29: 13000000 INFO @ Sun, 21 Jun 2020 23:48:31: 23000000 INFO @ Sun, 21 Jun 2020 23:48:33: 18000000 INFO @ Sun, 21 Jun 2020 23:48:36: 14000000 INFO @ Sun, 21 Jun 2020 23:48:38: 24000000 INFO @ Sun, 21 Jun 2020 23:48:40: 19000000 INFO @ Sun, 21 Jun 2020 23:48:43: 15000000 INFO @ Sun, 21 Jun 2020 23:48:44: 25000000 INFO @ Sun, 21 Jun 2020 23:48:47: 20000000 INFO @ Sun, 21 Jun 2020 23:48:50: 16000000 INFO @ Sun, 21 Jun 2020 23:48:51: 26000000 INFO @ Sun, 21 Jun 2020 23:48:53: 21000000 INFO @ Sun, 21 Jun 2020 23:48:57: 17000000 INFO @ Sun, 21 Jun 2020 23:48:58: 27000000 INFO @ Sun, 21 Jun 2020 23:49:00: 22000000 INFO @ Sun, 21 Jun 2020 23:49:03: 18000000 INFO @ Sun, 21 Jun 2020 23:49:05: 28000000 INFO @ Sun, 21 Jun 2020 23:49:07: 23000000 INFO @ Sun, 21 Jun 2020 23:49:10: 19000000 INFO @ Sun, 21 Jun 2020 23:49:11: 29000000 INFO @ Sun, 21 Jun 2020 23:49:13: 24000000 INFO @ Sun, 21 Jun 2020 23:49:17: 20000000 INFO @ Sun, 21 Jun 2020 23:49:18: 30000000 INFO @ Sun, 21 Jun 2020 23:49:20: 25000000 INFO @ Sun, 21 Jun 2020 23:49:23: 21000000 INFO @ Sun, 21 Jun 2020 23:49:25: 31000000 INFO @ Sun, 21 Jun 2020 23:49:27: 26000000 INFO @ Sun, 21 Jun 2020 23:49:30: 22000000 INFO @ Sun, 21 Jun 2020 23:49:31: 32000000 INFO @ Sun, 21 Jun 2020 23:49:34: 27000000 INFO @ Sun, 21 Jun 2020 23:49:36: 23000000 INFO @ Sun, 21 Jun 2020 23:49:38: 33000000 INFO @ Sun, 21 Jun 2020 23:49:41: 28000000 INFO @ Sun, 21 Jun 2020 23:49:44: 24000000 INFO @ Sun, 21 Jun 2020 23:49:46: 34000000 INFO @ Sun, 21 Jun 2020 23:49:48: 29000000 INFO @ Sun, 21 Jun 2020 23:49:52: 25000000 INFO @ Sun, 21 Jun 2020 23:49:53: 35000000 INFO @ Sun, 21 Jun 2020 23:49:55: 30000000 INFO @ Sun, 21 Jun 2020 23:49:59: 26000000 INFO @ Sun, 21 Jun 2020 23:50:00: 36000000 INFO @ Sun, 21 Jun 2020 23:50:02: 31000000 INFO @ Sun, 21 Jun 2020 23:50:07: 27000000 INFO @ Sun, 21 Jun 2020 23:50:07: 37000000 INFO @ Sun, 21 Jun 2020 23:50:09: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:50:14: 28000000 INFO @ Sun, 21 Jun 2020 23:50:14: 38000000 INFO @ Sun, 21 Jun 2020 23:50:17: 33000000 INFO @ Sun, 21 Jun 2020 23:50:21: 29000000 INFO @ Sun, 21 Jun 2020 23:50:21: 39000000 INFO @ Sun, 21 Jun 2020 23:50:24: 34000000 INFO @ Sun, 21 Jun 2020 23:50:28: 30000000 INFO @ Sun, 21 Jun 2020 23:50:29: 40000000 INFO @ Sun, 21 Jun 2020 23:50:31: 35000000 INFO @ Sun, 21 Jun 2020 23:50:35: 31000000 INFO @ Sun, 21 Jun 2020 23:50:36: 41000000 INFO @ Sun, 21 Jun 2020 23:50:38: 36000000 INFO @ Sun, 21 Jun 2020 23:50:42: 32000000 INFO @ Sun, 21 Jun 2020 23:50:42: 42000000 INFO @ Sun, 21 Jun 2020 23:50:45: 37000000 INFO @ Sun, 21 Jun 2020 23:50:49: 33000000 INFO @ Sun, 21 Jun 2020 23:50:49: 43000000 INFO @ Sun, 21 Jun 2020 23:50:52: 38000000 INFO @ Sun, 21 Jun 2020 23:50:56: 34000000 INFO @ Sun, 21 Jun 2020 23:50:56: 44000000 INFO @ Sun, 21 Jun 2020 23:50:59: 39000000 INFO @ Sun, 21 Jun 2020 23:51:03: 35000000 INFO @ Sun, 21 Jun 2020 23:51:03: 45000000 INFO @ Sun, 21 Jun 2020 23:51:06: 40000000 INFO @ Sun, 21 Jun 2020 23:51:10: 46000000 INFO @ Sun, 21 Jun 2020 23:51:10: 36000000 INFO @ Sun, 21 Jun 2020 23:51:13: 41000000 INFO @ Sun, 21 Jun 2020 23:51:17: 47000000 INFO @ Sun, 21 Jun 2020 23:51:17: 37000000 INFO @ Sun, 21 Jun 2020 23:51:20: 42000000 INFO @ Sun, 21 Jun 2020 23:51:22: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:51:22: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:51:22: #1 total tags in treatment: 47785921 INFO @ Sun, 21 Jun 2020 23:51:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:51:23: #1 tags after filtering in treatment: 47785876 INFO @ Sun, 21 Jun 2020 23:51:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:51:23: #1 finished! INFO @ Sun, 21 Jun 2020 23:51:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:51:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:51:24: 38000000 INFO @ Sun, 21 Jun 2020 23:51:26: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 23:51:26: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:51:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05_peaks.narrowPeak: No such file or directory INFO @ Sun, 21 Jun 2020 23:51:27: 43000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:51:31: 39000000 INFO @ Sun, 21 Jun 2020 23:51:33: 44000000 INFO @ Sun, 21 Jun 2020 23:51:37: 40000000 INFO @ Sun, 21 Jun 2020 23:51:40: 45000000 INFO @ Sun, 21 Jun 2020 23:51:44: 41000000 INFO @ Sun, 21 Jun 2020 23:51:47: 46000000 INFO @ Sun, 21 Jun 2020 23:51:51: 42000000 INFO @ Sun, 21 Jun 2020 23:51:54: 47000000 INFO @ Sun, 21 Jun 2020 23:51:58: 43000000 INFO @ Sun, 21 Jun 2020 23:51:59: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:51:59: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:51:59: #1 total tags in treatment: 47785921 INFO @ Sun, 21 Jun 2020 23:51:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:51:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:52:00: #1 tags after filtering in treatment: 47785876 INFO @ Sun, 21 Jun 2020 23:52:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:52:00: #1 finished! INFO @ Sun, 21 Jun 2020 23:52:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:52:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:52:03: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 23:52:03: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:52:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:52:04: 44000000 INFO @ Sun, 21 Jun 2020 23:52:11: 45000000 INFO @ Sun, 21 Jun 2020 23:52:17: 46000000 INFO @ Sun, 21 Jun 2020 23:52:23: 47000000 INFO @ Sun, 21 Jun 2020 23:52:29: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:52:29: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:52:29: #1 total tags in treatment: 47785921 INFO @ Sun, 21 Jun 2020 23:52:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:52:30: #1 tags after filtering in treatment: 47785876 INFO @ Sun, 21 Jun 2020 23:52:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:52:30: #1 finished! INFO @ Sun, 21 Jun 2020 23:52:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:52:33: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 23:52:33: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:52:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158310/SRX7158310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling