Job ID = 6459591 SRX = SRX7158308 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:12:19 prefetch.2.10.7: 1) Downloading 'SRR10467040'... 2020-06-21T13:12:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:24:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:24:47 prefetch.2.10.7: 1) 'SRR10467040' was downloaded successfully 2020-06-21T13:24:47 prefetch.2.10.7: 'SRR10467040' has 0 unresolved dependencies Read 74659995 spots for SRR10467040/SRR10467040.sra Written 74659995 spots for SRR10467040/SRR10467040.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:57 74659995 reads; of these: 74659995 (100.00%) were unpaired; of these: 4254057 (5.70%) aligned 0 times 46915783 (62.84%) aligned exactly 1 time 23490155 (31.46%) aligned >1 times 94.30% overall alignment rate Time searching: 00:35:58 Overall time: 00:35:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 18893687 / 70405938 = 0.2684 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:22:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:22:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:22:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:22:06: 1000000 INFO @ Sun, 21 Jun 2020 23:22:11: 2000000 INFO @ Sun, 21 Jun 2020 23:22:16: 3000000 INFO @ Sun, 21 Jun 2020 23:22:21: 4000000 INFO @ Sun, 21 Jun 2020 23:22:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:22:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:22:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:22:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:22:31: 6000000 INFO @ Sun, 21 Jun 2020 23:22:36: 1000000 INFO @ Sun, 21 Jun 2020 23:22:36: 7000000 INFO @ Sun, 21 Jun 2020 23:22:41: 2000000 INFO @ Sun, 21 Jun 2020 23:22:42: 8000000 INFO @ Sun, 21 Jun 2020 23:22:46: 3000000 INFO @ Sun, 21 Jun 2020 23:22:47: 9000000 INFO @ Sun, 21 Jun 2020 23:22:52: 4000000 INFO @ Sun, 21 Jun 2020 23:22:52: 10000000 INFO @ Sun, 21 Jun 2020 23:22:57: 5000000 INFO @ Sun, 21 Jun 2020 23:22:57: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:23:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:23:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:23:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:23:02: 6000000 INFO @ Sun, 21 Jun 2020 23:23:03: 12000000 INFO @ Sun, 21 Jun 2020 23:23:06: 1000000 INFO @ Sun, 21 Jun 2020 23:23:08: 7000000 INFO @ Sun, 21 Jun 2020 23:23:08: 13000000 INFO @ Sun, 21 Jun 2020 23:23:12: 2000000 INFO @ Sun, 21 Jun 2020 23:23:13: 8000000 INFO @ Sun, 21 Jun 2020 23:23:14: 14000000 INFO @ Sun, 21 Jun 2020 23:23:18: 3000000 INFO @ Sun, 21 Jun 2020 23:23:18: 9000000 INFO @ Sun, 21 Jun 2020 23:23:19: 15000000 INFO @ Sun, 21 Jun 2020 23:23:23: 4000000 INFO @ Sun, 21 Jun 2020 23:23:24: 10000000 INFO @ Sun, 21 Jun 2020 23:23:24: 16000000 INFO @ Sun, 21 Jun 2020 23:23:29: 5000000 INFO @ Sun, 21 Jun 2020 23:23:29: 11000000 INFO @ Sun, 21 Jun 2020 23:23:30: 17000000 INFO @ Sun, 21 Jun 2020 23:23:34: 6000000 INFO @ Sun, 21 Jun 2020 23:23:34: 12000000 INFO @ Sun, 21 Jun 2020 23:23:35: 18000000 INFO @ Sun, 21 Jun 2020 23:23:40: 7000000 INFO @ Sun, 21 Jun 2020 23:23:40: 13000000 INFO @ Sun, 21 Jun 2020 23:23:40: 19000000 INFO @ Sun, 21 Jun 2020 23:23:45: 14000000 INFO @ Sun, 21 Jun 2020 23:23:45: 8000000 INFO @ Sun, 21 Jun 2020 23:23:46: 20000000 INFO @ Sun, 21 Jun 2020 23:23:51: 15000000 INFO @ Sun, 21 Jun 2020 23:23:51: 21000000 INFO @ Sun, 21 Jun 2020 23:23:51: 9000000 INFO @ Sun, 21 Jun 2020 23:23:56: 22000000 INFO @ Sun, 21 Jun 2020 23:23:56: 16000000 INFO @ Sun, 21 Jun 2020 23:23:56: 10000000 INFO @ Sun, 21 Jun 2020 23:24:02: 23000000 INFO @ Sun, 21 Jun 2020 23:24:02: 17000000 INFO @ Sun, 21 Jun 2020 23:24:02: 11000000 INFO @ Sun, 21 Jun 2020 23:24:07: 24000000 INFO @ Sun, 21 Jun 2020 23:24:07: 18000000 INFO @ Sun, 21 Jun 2020 23:24:07: 12000000 INFO @ Sun, 21 Jun 2020 23:24:12: 25000000 INFO @ Sun, 21 Jun 2020 23:24:13: 19000000 INFO @ Sun, 21 Jun 2020 23:24:13: 13000000 INFO @ Sun, 21 Jun 2020 23:24:18: 20000000 INFO @ Sun, 21 Jun 2020 23:24:18: 26000000 INFO @ Sun, 21 Jun 2020 23:24:18: 14000000 INFO @ Sun, 21 Jun 2020 23:24:23: 21000000 INFO @ Sun, 21 Jun 2020 23:24:24: 27000000 INFO @ Sun, 21 Jun 2020 23:24:25: 15000000 INFO @ Sun, 21 Jun 2020 23:24:29: 22000000 INFO @ Sun, 21 Jun 2020 23:24:30: 28000000 INFO @ Sun, 21 Jun 2020 23:24:31: 16000000 INFO @ Sun, 21 Jun 2020 23:24:34: 23000000 INFO @ Sun, 21 Jun 2020 23:24:36: 29000000 INFO @ Sun, 21 Jun 2020 23:24:37: 17000000 INFO @ Sun, 21 Jun 2020 23:24:40: 24000000 INFO @ Sun, 21 Jun 2020 23:24:42: 30000000 INFO @ Sun, 21 Jun 2020 23:24:43: 18000000 INFO @ Sun, 21 Jun 2020 23:24:45: 25000000 INFO @ Sun, 21 Jun 2020 23:24:47: 31000000 INFO @ Sun, 21 Jun 2020 23:24:49: 19000000 INFO @ Sun, 21 Jun 2020 23:24:51: 26000000 INFO @ Sun, 21 Jun 2020 23:24:53: 32000000 INFO @ Sun, 21 Jun 2020 23:24:54: 20000000 INFO @ Sun, 21 Jun 2020 23:24:56: 27000000 INFO @ Sun, 21 Jun 2020 23:24:59: 33000000 INFO @ Sun, 21 Jun 2020 23:25:00: 21000000 INFO @ Sun, 21 Jun 2020 23:25:02: 28000000 INFO @ Sun, 21 Jun 2020 23:25:04: 34000000 INFO @ Sun, 21 Jun 2020 23:25:06: 22000000 INFO @ Sun, 21 Jun 2020 23:25:07: 29000000 INFO @ Sun, 21 Jun 2020 23:25:10: 35000000 INFO @ Sun, 21 Jun 2020 23:25:11: 23000000 INFO @ Sun, 21 Jun 2020 23:25:13: 30000000 INFO @ Sun, 21 Jun 2020 23:25:15: 36000000 INFO @ Sun, 21 Jun 2020 23:25:17: 24000000 INFO @ Sun, 21 Jun 2020 23:25:18: 31000000 INFO @ Sun, 21 Jun 2020 23:25:21: 37000000 INFO @ Sun, 21 Jun 2020 23:25:23: 25000000 INFO @ Sun, 21 Jun 2020 23:25:24: 32000000 INFO @ Sun, 21 Jun 2020 23:25:26: 38000000 INFO @ Sun, 21 Jun 2020 23:25:28: 26000000 INFO @ Sun, 21 Jun 2020 23:25:29: 33000000 INFO @ Sun, 21 Jun 2020 23:25:32: 39000000 INFO @ Sun, 21 Jun 2020 23:25:34: 27000000 INFO @ Sun, 21 Jun 2020 23:25:34: 34000000 INFO @ Sun, 21 Jun 2020 23:25:38: 40000000 INFO @ Sun, 21 Jun 2020 23:25:39: 35000000 INFO @ Sun, 21 Jun 2020 23:25:40: 28000000 INFO @ Sun, 21 Jun 2020 23:25:43: 41000000 INFO @ Sun, 21 Jun 2020 23:25:45: 36000000 INFO @ Sun, 21 Jun 2020 23:25:45: 29000000 INFO @ Sun, 21 Jun 2020 23:25:48: 42000000 INFO @ Sun, 21 Jun 2020 23:25:50: 37000000 INFO @ Sun, 21 Jun 2020 23:25:51: 30000000 INFO @ Sun, 21 Jun 2020 23:25:54: 43000000 INFO @ Sun, 21 Jun 2020 23:25:55: 38000000 INFO @ Sun, 21 Jun 2020 23:25:56: 31000000 INFO @ Sun, 21 Jun 2020 23:25:59: 44000000 INFO @ Sun, 21 Jun 2020 23:26:01: 39000000 INFO @ Sun, 21 Jun 2020 23:26:02: 32000000 INFO @ Sun, 21 Jun 2020 23:26:05: 45000000 INFO @ Sun, 21 Jun 2020 23:26:06: 40000000 INFO @ Sun, 21 Jun 2020 23:26:07: 33000000 INFO @ Sun, 21 Jun 2020 23:26:10: 46000000 INFO @ Sun, 21 Jun 2020 23:26:12: 41000000 INFO @ Sun, 21 Jun 2020 23:26:13: 34000000 INFO @ Sun, 21 Jun 2020 23:26:15: 47000000 INFO @ Sun, 21 Jun 2020 23:26:17: 42000000 INFO @ Sun, 21 Jun 2020 23:26:18: 35000000 INFO @ Sun, 21 Jun 2020 23:26:21: 48000000 INFO @ Sun, 21 Jun 2020 23:26:22: 43000000 INFO @ Sun, 21 Jun 2020 23:26:24: 36000000 INFO @ Sun, 21 Jun 2020 23:26:26: 49000000 INFO @ Sun, 21 Jun 2020 23:26:28: 44000000 INFO @ Sun, 21 Jun 2020 23:26:29: 37000000 INFO @ Sun, 21 Jun 2020 23:26:32: 50000000 INFO @ Sun, 21 Jun 2020 23:26:33: 45000000 INFO @ Sun, 21 Jun 2020 23:26:35: 38000000 INFO @ Sun, 21 Jun 2020 23:26:37: 51000000 INFO @ Sun, 21 Jun 2020 23:26:38: 46000000 INFO @ Sun, 21 Jun 2020 23:26:40: 39000000 INFO @ Sun, 21 Jun 2020 23:26:40: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:26:40: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:26:40: #1 total tags in treatment: 51512251 INFO @ Sun, 21 Jun 2020 23:26:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:26:41: #1 tags after filtering in treatment: 51512200 INFO @ Sun, 21 Jun 2020 23:26:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:26:41: #1 finished! INFO @ Sun, 21 Jun 2020 23:26:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:26:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:26:43: 47000000 INFO @ Sun, 21 Jun 2020 23:26:44: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:26:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:26:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:26:46: 40000000 INFO @ Sun, 21 Jun 2020 23:26:48: 48000000 INFO @ Sun, 21 Jun 2020 23:26:51: 41000000 INFO @ Sun, 21 Jun 2020 23:26:54: 49000000 INFO @ Sun, 21 Jun 2020 23:26:56: 42000000 INFO @ Sun, 21 Jun 2020 23:26:59: 50000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:27:01: 43000000 INFO @ Sun, 21 Jun 2020 23:27:04: 51000000 INFO @ Sun, 21 Jun 2020 23:27:07: 44000000 INFO @ Sun, 21 Jun 2020 23:27:07: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:27:07: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:27:07: #1 total tags in treatment: 51512251 INFO @ Sun, 21 Jun 2020 23:27:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:27:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:27:08: #1 tags after filtering in treatment: 51512200 INFO @ Sun, 21 Jun 2020 23:27:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:27:08: #1 finished! INFO @ Sun, 21 Jun 2020 23:27:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:27:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:27:11: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:27:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:27:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:27:12: 45000000 INFO @ Sun, 21 Jun 2020 23:27:17: 46000000 INFO @ Sun, 21 Jun 2020 23:27:22: 47000000 INFO @ Sun, 21 Jun 2020 23:27:27: 48000000 INFO @ Sun, 21 Jun 2020 23:27:32: 49000000 INFO @ Sun, 21 Jun 2020 23:27:37: 50000000 INFO @ Sun, 21 Jun 2020 23:27:42: 51000000 INFO @ Sun, 21 Jun 2020 23:27:45: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:27:45: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:27:45: #1 total tags in treatment: 51512251 INFO @ Sun, 21 Jun 2020 23:27:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:27:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:27:46: #1 tags after filtering in treatment: 51512200 INFO @ Sun, 21 Jun 2020 23:27:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:27:46: #1 finished! INFO @ Sun, 21 Jun 2020 23:27:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:27:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:27:49: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:27:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:27:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158308/SRX7158308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。