Job ID = 6459588 SRX = SRX7158304 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:16:50 prefetch.2.10.7: 1) Downloading 'SRR10467044'... 2020-06-21T13:16:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:27:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:27:45 prefetch.2.10.7: 1) 'SRR10467044' was downloaded successfully 2020-06-21T13:27:45 prefetch.2.10.7: 'SRR10467044' has 0 unresolved dependencies Read 74708824 spots for SRR10467044/SRR10467044.sra Written 74708824 spots for SRR10467044/SRR10467044.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:43 74708824 reads; of these: 74708824 (100.00%) were unpaired; of these: 3320281 (4.44%) aligned 0 times 62174063 (83.22%) aligned exactly 1 time 9214480 (12.33%) aligned >1 times 95.56% overall alignment rate Time searching: 00:26:43 Overall time: 00:26:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 18628367 / 71388543 = 0.2609 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:16:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:16:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:16:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:16:31: 1000000 INFO @ Sun, 21 Jun 2020 23:16:37: 2000000 INFO @ Sun, 21 Jun 2020 23:16:43: 3000000 INFO @ Sun, 21 Jun 2020 23:16:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:16:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:16:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:16:55: 5000000 INFO @ Sun, 21 Jun 2020 23:17:01: 1000000 INFO @ Sun, 21 Jun 2020 23:17:02: 6000000 INFO @ Sun, 21 Jun 2020 23:17:09: 2000000 INFO @ Sun, 21 Jun 2020 23:17:10: 7000000 INFO @ Sun, 21 Jun 2020 23:17:16: 3000000 INFO @ Sun, 21 Jun 2020 23:17:17: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:17:23: 4000000 INFO @ Sun, 21 Jun 2020 23:17:24: 9000000 INFO @ Sun, 21 Jun 2020 23:17:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:17:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:17:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:17:31: 5000000 INFO @ Sun, 21 Jun 2020 23:17:32: 10000000 INFO @ Sun, 21 Jun 2020 23:17:32: 1000000 INFO @ Sun, 21 Jun 2020 23:17:38: 6000000 INFO @ Sun, 21 Jun 2020 23:17:40: 11000000 INFO @ Sun, 21 Jun 2020 23:17:40: 2000000 INFO @ Sun, 21 Jun 2020 23:17:46: 7000000 INFO @ Sun, 21 Jun 2020 23:17:47: 12000000 INFO @ Sun, 21 Jun 2020 23:17:48: 3000000 INFO @ Sun, 21 Jun 2020 23:17:54: 8000000 INFO @ Sun, 21 Jun 2020 23:17:55: 13000000 INFO @ Sun, 21 Jun 2020 23:17:55: 4000000 INFO @ Sun, 21 Jun 2020 23:18:01: 9000000 INFO @ Sun, 21 Jun 2020 23:18:02: 14000000 INFO @ Sun, 21 Jun 2020 23:18:03: 5000000 INFO @ Sun, 21 Jun 2020 23:18:09: 10000000 INFO @ Sun, 21 Jun 2020 23:18:10: 15000000 INFO @ Sun, 21 Jun 2020 23:18:10: 6000000 INFO @ Sun, 21 Jun 2020 23:18:16: 11000000 INFO @ Sun, 21 Jun 2020 23:18:17: 16000000 INFO @ Sun, 21 Jun 2020 23:18:18: 7000000 INFO @ Sun, 21 Jun 2020 23:18:24: 12000000 INFO @ Sun, 21 Jun 2020 23:18:25: 17000000 INFO @ Sun, 21 Jun 2020 23:18:26: 8000000 INFO @ Sun, 21 Jun 2020 23:18:32: 13000000 INFO @ Sun, 21 Jun 2020 23:18:33: 18000000 INFO @ Sun, 21 Jun 2020 23:18:35: 9000000 INFO @ Sun, 21 Jun 2020 23:18:40: 14000000 INFO @ Sun, 21 Jun 2020 23:18:41: 19000000 INFO @ Sun, 21 Jun 2020 23:18:44: 10000000 INFO @ Sun, 21 Jun 2020 23:18:48: 15000000 INFO @ Sun, 21 Jun 2020 23:18:49: 20000000 INFO @ Sun, 21 Jun 2020 23:18:52: 11000000 INFO @ Sun, 21 Jun 2020 23:18:56: 16000000 INFO @ Sun, 21 Jun 2020 23:18:57: 21000000 INFO @ Sun, 21 Jun 2020 23:19:00: 12000000 INFO @ Sun, 21 Jun 2020 23:19:04: 17000000 INFO @ Sun, 21 Jun 2020 23:19:05: 22000000 INFO @ Sun, 21 Jun 2020 23:19:08: 13000000 INFO @ Sun, 21 Jun 2020 23:19:11: 18000000 INFO @ Sun, 21 Jun 2020 23:19:13: 23000000 INFO @ Sun, 21 Jun 2020 23:19:16: 14000000 INFO @ Sun, 21 Jun 2020 23:19:19: 19000000 INFO @ Sun, 21 Jun 2020 23:19:21: 24000000 INFO @ Sun, 21 Jun 2020 23:19:24: 15000000 INFO @ Sun, 21 Jun 2020 23:19:27: 20000000 INFO @ Sun, 21 Jun 2020 23:19:29: 25000000 INFO @ Sun, 21 Jun 2020 23:19:32: 16000000 INFO @ Sun, 21 Jun 2020 23:19:35: 21000000 INFO @ Sun, 21 Jun 2020 23:19:37: 26000000 INFO @ Sun, 21 Jun 2020 23:19:40: 17000000 INFO @ Sun, 21 Jun 2020 23:19:42: 22000000 INFO @ Sun, 21 Jun 2020 23:19:45: 27000000 INFO @ Sun, 21 Jun 2020 23:19:48: 18000000 INFO @ Sun, 21 Jun 2020 23:19:50: 23000000 INFO @ Sun, 21 Jun 2020 23:19:52: 28000000 INFO @ Sun, 21 Jun 2020 23:19:56: 19000000 INFO @ Sun, 21 Jun 2020 23:19:58: 24000000 INFO @ Sun, 21 Jun 2020 23:20:00: 29000000 INFO @ Sun, 21 Jun 2020 23:20:04: 20000000 INFO @ Sun, 21 Jun 2020 23:20:05: 25000000 INFO @ Sun, 21 Jun 2020 23:20:09: 30000000 INFO @ Sun, 21 Jun 2020 23:20:12: 21000000 INFO @ Sun, 21 Jun 2020 23:20:13: 26000000 INFO @ Sun, 21 Jun 2020 23:20:16: 31000000 INFO @ Sun, 21 Jun 2020 23:20:20: 22000000 INFO @ Sun, 21 Jun 2020 23:20:21: 27000000 INFO @ Sun, 21 Jun 2020 23:20:24: 32000000 INFO @ Sun, 21 Jun 2020 23:20:28: 23000000 INFO @ Sun, 21 Jun 2020 23:20:28: 28000000 INFO @ Sun, 21 Jun 2020 23:20:32: 33000000 INFO @ Sun, 21 Jun 2020 23:20:35: 24000000 INFO @ Sun, 21 Jun 2020 23:20:36: 29000000 INFO @ Sun, 21 Jun 2020 23:20:40: 34000000 INFO @ Sun, 21 Jun 2020 23:20:43: 25000000 INFO @ Sun, 21 Jun 2020 23:20:44: 30000000 INFO @ Sun, 21 Jun 2020 23:20:47: 35000000 INFO @ Sun, 21 Jun 2020 23:20:51: 26000000 INFO @ Sun, 21 Jun 2020 23:20:51: 31000000 INFO @ Sun, 21 Jun 2020 23:20:55: 36000000 INFO @ Sun, 21 Jun 2020 23:20:58: 27000000 INFO @ Sun, 21 Jun 2020 23:20:58: 32000000 INFO @ Sun, 21 Jun 2020 23:21:03: 37000000 INFO @ Sun, 21 Jun 2020 23:21:06: 28000000 INFO @ Sun, 21 Jun 2020 23:21:06: 33000000 INFO @ Sun, 21 Jun 2020 23:21:10: 38000000 INFO @ Sun, 21 Jun 2020 23:21:13: 34000000 INFO @ Sun, 21 Jun 2020 23:21:13: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:21:18: 39000000 INFO @ Sun, 21 Jun 2020 23:21:21: 35000000 INFO @ Sun, 21 Jun 2020 23:21:21: 30000000 INFO @ Sun, 21 Jun 2020 23:21:26: 40000000 INFO @ Sun, 21 Jun 2020 23:21:28: 36000000 INFO @ Sun, 21 Jun 2020 23:21:29: 31000000 INFO @ Sun, 21 Jun 2020 23:21:33: 41000000 INFO @ Sun, 21 Jun 2020 23:21:36: 37000000 INFO @ Sun, 21 Jun 2020 23:21:37: 32000000 INFO @ Sun, 21 Jun 2020 23:21:41: 42000000 INFO @ Sun, 21 Jun 2020 23:21:44: 38000000 INFO @ Sun, 21 Jun 2020 23:21:44: 33000000 INFO @ Sun, 21 Jun 2020 23:21:49: 43000000 INFO @ Sun, 21 Jun 2020 23:21:51: 39000000 INFO @ Sun, 21 Jun 2020 23:21:52: 34000000 INFO @ Sun, 21 Jun 2020 23:21:57: 44000000 INFO @ Sun, 21 Jun 2020 23:21:58: 40000000 INFO @ Sun, 21 Jun 2020 23:22:00: 35000000 INFO @ Sun, 21 Jun 2020 23:22:04: 45000000 INFO @ Sun, 21 Jun 2020 23:22:06: 41000000 INFO @ Sun, 21 Jun 2020 23:22:07: 36000000 INFO @ Sun, 21 Jun 2020 23:22:12: 46000000 INFO @ Sun, 21 Jun 2020 23:22:14: 42000000 INFO @ Sun, 21 Jun 2020 23:22:16: 37000000 INFO @ Sun, 21 Jun 2020 23:22:20: 47000000 INFO @ Sun, 21 Jun 2020 23:22:22: 43000000 INFO @ Sun, 21 Jun 2020 23:22:24: 38000000 INFO @ Sun, 21 Jun 2020 23:22:28: 48000000 INFO @ Sun, 21 Jun 2020 23:22:29: 44000000 INFO @ Sun, 21 Jun 2020 23:22:31: 39000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:22:36: 49000000 INFO @ Sun, 21 Jun 2020 23:22:37: 45000000 INFO @ Sun, 21 Jun 2020 23:22:39: 40000000 INFO @ Sun, 21 Jun 2020 23:22:44: 50000000 INFO @ Sun, 21 Jun 2020 23:22:44: 46000000 INFO @ Sun, 21 Jun 2020 23:22:47: 41000000 INFO @ Sun, 21 Jun 2020 23:22:51: 51000000 INFO @ Sun, 21 Jun 2020 23:22:52: 47000000 INFO @ Sun, 21 Jun 2020 23:22:54: 42000000 INFO @ Sun, 21 Jun 2020 23:22:59: 52000000 INFO @ Sun, 21 Jun 2020 23:22:59: 48000000 INFO @ Sun, 21 Jun 2020 23:23:02: 43000000 INFO @ Sun, 21 Jun 2020 23:23:05: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:23:05: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:23:05: #1 total tags in treatment: 52760176 INFO @ Sun, 21 Jun 2020 23:23:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:23:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:23:06: #1 tags after filtering in treatment: 52760094 INFO @ Sun, 21 Jun 2020 23:23:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:23:06: #1 finished! INFO @ Sun, 21 Jun 2020 23:23:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:23:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:23:07: 49000000 INFO @ Sun, 21 Jun 2020 23:23:09: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:23:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:23:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:23:10: 44000000 INFO @ Sun, 21 Jun 2020 23:23:14: 50000000 INFO @ Sun, 21 Jun 2020 23:23:17: 45000000 INFO @ Sun, 21 Jun 2020 23:23:21: 51000000 INFO @ Sun, 21 Jun 2020 23:23:25: 46000000 INFO @ Sun, 21 Jun 2020 23:23:29: 52000000 INFO @ Sun, 21 Jun 2020 23:23:32: 47000000 INFO @ Sun, 21 Jun 2020 23:23:35: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:23:35: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:23:35: #1 total tags in treatment: 52760176 INFO @ Sun, 21 Jun 2020 23:23:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:23:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:23:36: #1 tags after filtering in treatment: 52760094 INFO @ Sun, 21 Jun 2020 23:23:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:23:36: #1 finished! INFO @ Sun, 21 Jun 2020 23:23:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:23:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:23:39: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:23:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:23:39: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 23:23:39: 48000000 cut: /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:23:46: 49000000 INFO @ Sun, 21 Jun 2020 23:23:53: 50000000 INFO @ Sun, 21 Jun 2020 23:23:59: 51000000 INFO @ Sun, 21 Jun 2020 23:24:06: 52000000 INFO @ Sun, 21 Jun 2020 23:24:11: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 23:24:11: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 23:24:11: #1 total tags in treatment: 52760176 INFO @ Sun, 21 Jun 2020 23:24:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:24:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:24:12: #1 tags after filtering in treatment: 52760094 INFO @ Sun, 21 Jun 2020 23:24:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:24:12: #1 finished! INFO @ Sun, 21 Jun 2020 23:24:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:24:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:24:16: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:24:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:24:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX7158304/SRX7158304.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling