Job ID = 6509165 SRX = SRX699542 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T15:07:29 prefetch.2.10.7: 1) Downloading 'SRR1573541'... 2020-06-26T15:07:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T15:10:23 prefetch.2.10.7: HTTPS download succeed 2020-06-26T15:10:23 prefetch.2.10.7: 1) 'SRR1573541' was downloaded successfully Read 48706360 spots for SRR1573541/SRR1573541.sra Written 48706360 spots for SRR1573541/SRR1573541.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:54 48706360 reads; of these: 48706360 (100.00%) were unpaired; of these: 8662757 (17.79%) aligned 0 times 36367679 (74.67%) aligned exactly 1 time 3675924 (7.55%) aligned >1 times 82.21% overall alignment rate Time searching: 00:07:54 Overall time: 00:07:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4767917 / 40043603 = 0.1191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:29:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:29:30: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:29:30: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:29:36: 1000000 INFO @ Sat, 27 Jun 2020 00:29:41: 2000000 INFO @ Sat, 27 Jun 2020 00:29:47: 3000000 INFO @ Sat, 27 Jun 2020 00:29:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:29:59: 5000000 INFO @ Sat, 27 Jun 2020 00:30:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:00: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:00: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:06: 6000000 INFO @ Sat, 27 Jun 2020 00:30:06: 1000000 INFO @ Sat, 27 Jun 2020 00:30:12: 7000000 INFO @ Sat, 27 Jun 2020 00:30:13: 2000000 INFO @ Sat, 27 Jun 2020 00:30:19: 8000000 INFO @ Sat, 27 Jun 2020 00:30:20: 3000000 INFO @ Sat, 27 Jun 2020 00:30:25: 9000000 INFO @ Sat, 27 Jun 2020 00:30:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:30:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:30:30: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:30:30: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:30:32: 10000000 INFO @ Sat, 27 Jun 2020 00:30:33: 5000000 INFO @ Sat, 27 Jun 2020 00:30:37: 1000000 INFO @ Sat, 27 Jun 2020 00:30:39: 11000000 INFO @ Sat, 27 Jun 2020 00:30:40: 6000000 INFO @ Sat, 27 Jun 2020 00:30:44: 2000000 INFO @ Sat, 27 Jun 2020 00:30:46: 12000000 INFO @ Sat, 27 Jun 2020 00:30:47: 7000000 INFO @ Sat, 27 Jun 2020 00:30:52: 3000000 INFO @ Sat, 27 Jun 2020 00:30:53: 13000000 INFO @ Sat, 27 Jun 2020 00:30:54: 8000000 INFO @ Sat, 27 Jun 2020 00:30:59: 4000000 INFO @ Sat, 27 Jun 2020 00:31:00: 14000000 INFO @ Sat, 27 Jun 2020 00:31:01: 9000000 INFO @ Sat, 27 Jun 2020 00:31:06: 5000000 INFO @ Sat, 27 Jun 2020 00:31:06: 15000000 INFO @ Sat, 27 Jun 2020 00:31:08: 10000000 INFO @ Sat, 27 Jun 2020 00:31:13: 16000000 INFO @ Sat, 27 Jun 2020 00:31:13: 6000000 INFO @ Sat, 27 Jun 2020 00:31:14: 11000000 INFO @ Sat, 27 Jun 2020 00:31:19: 17000000 INFO @ Sat, 27 Jun 2020 00:31:20: 7000000 INFO @ Sat, 27 Jun 2020 00:31:21: 12000000 INFO @ Sat, 27 Jun 2020 00:31:26: 18000000 INFO @ Sat, 27 Jun 2020 00:31:27: 8000000 INFO @ Sat, 27 Jun 2020 00:31:28: 13000000 INFO @ Sat, 27 Jun 2020 00:31:33: 19000000 INFO @ Sat, 27 Jun 2020 00:31:34: 9000000 INFO @ Sat, 27 Jun 2020 00:31:35: 14000000 INFO @ Sat, 27 Jun 2020 00:31:39: 20000000 INFO @ Sat, 27 Jun 2020 00:31:41: 10000000 INFO @ Sat, 27 Jun 2020 00:31:42: 15000000 INFO @ Sat, 27 Jun 2020 00:31:46: 21000000 INFO @ Sat, 27 Jun 2020 00:31:48: 11000000 INFO @ Sat, 27 Jun 2020 00:31:48: 16000000 INFO @ Sat, 27 Jun 2020 00:31:53: 22000000 INFO @ Sat, 27 Jun 2020 00:31:55: 12000000 INFO @ Sat, 27 Jun 2020 00:31:55: 17000000 INFO @ Sat, 27 Jun 2020 00:31:59: 23000000 INFO @ Sat, 27 Jun 2020 00:32:02: 18000000 INFO @ Sat, 27 Jun 2020 00:32:02: 13000000 INFO @ Sat, 27 Jun 2020 00:32:06: 24000000 INFO @ Sat, 27 Jun 2020 00:32:10: 19000000 INFO @ Sat, 27 Jun 2020 00:32:10: 14000000 INFO @ Sat, 27 Jun 2020 00:32:14: 25000000 INFO @ Sat, 27 Jun 2020 00:32:17: 20000000 INFO @ Sat, 27 Jun 2020 00:32:18: 15000000 INFO @ Sat, 27 Jun 2020 00:32:21: 26000000 INFO @ Sat, 27 Jun 2020 00:32:24: 21000000 INFO @ Sat, 27 Jun 2020 00:32:25: 16000000 INFO @ Sat, 27 Jun 2020 00:32:27: 27000000 INFO @ Sat, 27 Jun 2020 00:32:31: 22000000 INFO @ Sat, 27 Jun 2020 00:32:31: 17000000 INFO @ Sat, 27 Jun 2020 00:32:34: 28000000 INFO @ Sat, 27 Jun 2020 00:32:37: 23000000 INFO @ Sat, 27 Jun 2020 00:32:38: 18000000 INFO @ Sat, 27 Jun 2020 00:32:41: 29000000 INFO @ Sat, 27 Jun 2020 00:32:44: 24000000 INFO @ Sat, 27 Jun 2020 00:32:45: 19000000 INFO @ Sat, 27 Jun 2020 00:32:48: 30000000 INFO @ Sat, 27 Jun 2020 00:32:51: 25000000 INFO @ Sat, 27 Jun 2020 00:32:52: 20000000 INFO @ Sat, 27 Jun 2020 00:32:55: 31000000 INFO @ Sat, 27 Jun 2020 00:32:58: 26000000 INFO @ Sat, 27 Jun 2020 00:32:58: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:33:01: 32000000 INFO @ Sat, 27 Jun 2020 00:33:04: 27000000 INFO @ Sat, 27 Jun 2020 00:33:05: 22000000 INFO @ Sat, 27 Jun 2020 00:33:08: 33000000 INFO @ Sat, 27 Jun 2020 00:33:11: 28000000 INFO @ Sat, 27 Jun 2020 00:33:11: 23000000 INFO @ Sat, 27 Jun 2020 00:33:15: 34000000 INFO @ Sat, 27 Jun 2020 00:33:18: 24000000 INFO @ Sat, 27 Jun 2020 00:33:18: 29000000 INFO @ Sat, 27 Jun 2020 00:33:21: 35000000 INFO @ Sat, 27 Jun 2020 00:33:24: #1 tag size is determined as 36 bps INFO @ Sat, 27 Jun 2020 00:33:24: #1 tag size = 36 INFO @ Sat, 27 Jun 2020 00:33:24: #1 total tags in treatment: 35275686 INFO @ Sat, 27 Jun 2020 00:33:24: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:33:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:33:24: #1 tags after filtering in treatment: 35275612 INFO @ Sat, 27 Jun 2020 00:33:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:33:24: #1 finished! INFO @ Sat, 27 Jun 2020 00:33:24: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:33:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:33:25: 25000000 INFO @ Sat, 27 Jun 2020 00:33:25: 30000000 INFO @ Sat, 27 Jun 2020 00:33:27: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:33:27: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:33:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:33:31: 26000000 INFO @ Sat, 27 Jun 2020 00:33:32: 31000000 INFO @ Sat, 27 Jun 2020 00:33:38: 27000000 INFO @ Sat, 27 Jun 2020 00:33:38: 32000000 INFO @ Sat, 27 Jun 2020 00:33:44: 28000000 INFO @ Sat, 27 Jun 2020 00:33:45: 33000000 INFO @ Sat, 27 Jun 2020 00:33:51: 29000000 INFO @ Sat, 27 Jun 2020 00:33:52: 34000000 INFO @ Sat, 27 Jun 2020 00:33:59: 30000000 INFO @ Sat, 27 Jun 2020 00:33:59: 35000000 INFO @ Sat, 27 Jun 2020 00:34:02: #1 tag size is determined as 36 bps INFO @ Sat, 27 Jun 2020 00:34:02: #1 tag size = 36 INFO @ Sat, 27 Jun 2020 00:34:02: #1 total tags in treatment: 35275686 INFO @ Sat, 27 Jun 2020 00:34:02: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:34:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:34:03: #1 tags after filtering in treatment: 35275612 INFO @ Sat, 27 Jun 2020 00:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:34:03: #1 finished! INFO @ Sat, 27 Jun 2020 00:34:03: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:34:05: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:34:05: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:34:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:34:06: 31000000 INFO @ Sat, 27 Jun 2020 00:34:12: 32000000 INFO @ Sat, 27 Jun 2020 00:34:18: 33000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:34:24: 34000000 INFO @ Sat, 27 Jun 2020 00:34:30: 35000000 INFO @ Sat, 27 Jun 2020 00:34:33: #1 tag size is determined as 36 bps INFO @ Sat, 27 Jun 2020 00:34:33: #1 tag size = 36 INFO @ Sat, 27 Jun 2020 00:34:33: #1 total tags in treatment: 35275686 INFO @ Sat, 27 Jun 2020 00:34:33: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:34:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:34:33: #1 tags after filtering in treatment: 35275612 INFO @ Sat, 27 Jun 2020 00:34:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 27 Jun 2020 00:34:33: #1 finished! INFO @ Sat, 27 Jun 2020 00:34:33: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:34:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:34:36: #2 number of paired peaks: 21 WARNING @ Sat, 27 Jun 2020 00:34:36: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 27 Jun 2020 00:34:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX699542/SRX699542.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling