Job ID = 6459474 SRX = SRX6827252 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:17:19 prefetch.2.10.7: 1) Downloading 'SRR10094665'... 2020-06-21T13:17:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:14 prefetch.2.10.7: 1) 'SRR10094665' was downloaded successfully Read 122784627 spots for SRR10094665/SRR10094665.sra Written 122784627 spots for SRR10094665/SRR10094665.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:30 122784627 reads; of these: 122784627 (100.00%) were unpaired; of these: 37249919 (30.34%) aligned 0 times 73803585 (60.11%) aligned exactly 1 time 11731123 (9.55%) aligned >1 times 69.66% overall alignment rate Time searching: 00:30:30 Overall time: 00:30:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 34753953 / 85534708 = 0.4063 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:32:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:32:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:32:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:32:34: 1000000 INFO @ Sun, 21 Jun 2020 23:32:40: 2000000 INFO @ Sun, 21 Jun 2020 23:32:46: 3000000 INFO @ Sun, 21 Jun 2020 23:32:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:32:58: 5000000 INFO @ Sun, 21 Jun 2020 23:32:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:32:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:32:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:33:05: 6000000 INFO @ Sun, 21 Jun 2020 23:33:05: 1000000 INFO @ Sun, 21 Jun 2020 23:33:12: 7000000 INFO @ Sun, 21 Jun 2020 23:33:12: 2000000 INFO @ Sun, 21 Jun 2020 23:33:19: 8000000 INFO @ Sun, 21 Jun 2020 23:33:19: 3000000 INFO @ Sun, 21 Jun 2020 23:33:26: 9000000 INFO @ Sun, 21 Jun 2020 23:33:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:33:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:33:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:33:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:33:33: 10000000 INFO @ Sun, 21 Jun 2020 23:33:33: 5000000 INFO @ Sun, 21 Jun 2020 23:33:35: 1000000 INFO @ Sun, 21 Jun 2020 23:33:40: 11000000 INFO @ Sun, 21 Jun 2020 23:33:40: 6000000 INFO @ Sun, 21 Jun 2020 23:33:42: 2000000 INFO @ Sun, 21 Jun 2020 23:33:47: 12000000 INFO @ Sun, 21 Jun 2020 23:33:47: 7000000 INFO @ Sun, 21 Jun 2020 23:33:49: 3000000 INFO @ Sun, 21 Jun 2020 23:33:54: 13000000 INFO @ Sun, 21 Jun 2020 23:33:54: 8000000 INFO @ Sun, 21 Jun 2020 23:33:56: 4000000 INFO @ Sun, 21 Jun 2020 23:34:00: 14000000 INFO @ Sun, 21 Jun 2020 23:34:01: 9000000 INFO @ Sun, 21 Jun 2020 23:34:03: 5000000 INFO @ Sun, 21 Jun 2020 23:34:07: 15000000 INFO @ Sun, 21 Jun 2020 23:34:08: 10000000 INFO @ Sun, 21 Jun 2020 23:34:10: 6000000 INFO @ Sun, 21 Jun 2020 23:34:14: 16000000 INFO @ Sun, 21 Jun 2020 23:34:15: 11000000 INFO @ Sun, 21 Jun 2020 23:34:17: 7000000 INFO @ Sun, 21 Jun 2020 23:34:21: 17000000 INFO @ Sun, 21 Jun 2020 23:34:22: 12000000 INFO @ Sun, 21 Jun 2020 23:34:24: 8000000 INFO @ Sun, 21 Jun 2020 23:34:28: 18000000 INFO @ Sun, 21 Jun 2020 23:34:29: 13000000 INFO @ Sun, 21 Jun 2020 23:34:31: 9000000 INFO @ Sun, 21 Jun 2020 23:34:35: 19000000 INFO @ Sun, 21 Jun 2020 23:34:36: 14000000 INFO @ Sun, 21 Jun 2020 23:34:38: 10000000 INFO @ Sun, 21 Jun 2020 23:34:42: 20000000 INFO @ Sun, 21 Jun 2020 23:34:43: 15000000 INFO @ Sun, 21 Jun 2020 23:34:44: 11000000 INFO @ Sun, 21 Jun 2020 23:34:49: 21000000 INFO @ Sun, 21 Jun 2020 23:34:50: 16000000 INFO @ Sun, 21 Jun 2020 23:34:51: 12000000 INFO @ Sun, 21 Jun 2020 23:34:56: 22000000 INFO @ Sun, 21 Jun 2020 23:34:56: 17000000 INFO @ Sun, 21 Jun 2020 23:34:58: 13000000 INFO @ Sun, 21 Jun 2020 23:35:03: 23000000 INFO @ Sun, 21 Jun 2020 23:35:03: 18000000 INFO @ Sun, 21 Jun 2020 23:35:05: 14000000 INFO @ Sun, 21 Jun 2020 23:35:10: 24000000 INFO @ Sun, 21 Jun 2020 23:35:10: 19000000 INFO @ Sun, 21 Jun 2020 23:35:12: 15000000 INFO @ Sun, 21 Jun 2020 23:35:17: 25000000 INFO @ Sun, 21 Jun 2020 23:35:17: 20000000 INFO @ Sun, 21 Jun 2020 23:35:19: 16000000 INFO @ Sun, 21 Jun 2020 23:35:24: 26000000 INFO @ Sun, 21 Jun 2020 23:35:24: 21000000 INFO @ Sun, 21 Jun 2020 23:35:25: 17000000 INFO @ Sun, 21 Jun 2020 23:35:31: 27000000 INFO @ Sun, 21 Jun 2020 23:35:31: 22000000 INFO @ Sun, 21 Jun 2020 23:35:32: 18000000 INFO @ Sun, 21 Jun 2020 23:35:38: 28000000 INFO @ Sun, 21 Jun 2020 23:35:38: 23000000 INFO @ Sun, 21 Jun 2020 23:35:39: 19000000 INFO @ Sun, 21 Jun 2020 23:35:45: 29000000 INFO @ Sun, 21 Jun 2020 23:35:45: 24000000 INFO @ Sun, 21 Jun 2020 23:35:46: 20000000 INFO @ Sun, 21 Jun 2020 23:35:52: 30000000 INFO @ Sun, 21 Jun 2020 23:35:52: 25000000 INFO @ Sun, 21 Jun 2020 23:35:53: 21000000 INFO @ Sun, 21 Jun 2020 23:35:59: 31000000 INFO @ Sun, 21 Jun 2020 23:35:59: 26000000 INFO @ Sun, 21 Jun 2020 23:36:00: 22000000 INFO @ Sun, 21 Jun 2020 23:36:06: 32000000 INFO @ Sun, 21 Jun 2020 23:36:06: 27000000 INFO @ Sun, 21 Jun 2020 23:36:06: 23000000 INFO @ Sun, 21 Jun 2020 23:36:13: 33000000 INFO @ Sun, 21 Jun 2020 23:36:13: 28000000 INFO @ Sun, 21 Jun 2020 23:36:13: 24000000 INFO @ Sun, 21 Jun 2020 23:36:20: 34000000 INFO @ Sun, 21 Jun 2020 23:36:20: 29000000 INFO @ Sun, 21 Jun 2020 23:36:20: 25000000 INFO @ Sun, 21 Jun 2020 23:36:27: 35000000 INFO @ Sun, 21 Jun 2020 23:36:27: 30000000 INFO @ Sun, 21 Jun 2020 23:36:27: 26000000 INFO @ Sun, 21 Jun 2020 23:36:33: 36000000 INFO @ Sun, 21 Jun 2020 23:36:34: 31000000 INFO @ Sun, 21 Jun 2020 23:36:34: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:36:40: 37000000 INFO @ Sun, 21 Jun 2020 23:36:41: 32000000 INFO @ Sun, 21 Jun 2020 23:36:41: 28000000 INFO @ Sun, 21 Jun 2020 23:36:47: 38000000 INFO @ Sun, 21 Jun 2020 23:36:48: 33000000 INFO @ Sun, 21 Jun 2020 23:36:48: 29000000 INFO @ Sun, 21 Jun 2020 23:36:54: 39000000 INFO @ Sun, 21 Jun 2020 23:36:54: 34000000 INFO @ Sun, 21 Jun 2020 23:36:55: 30000000 INFO @ Sun, 21 Jun 2020 23:37:01: 35000000 INFO @ Sun, 21 Jun 2020 23:37:02: 40000000 INFO @ Sun, 21 Jun 2020 23:37:02: 31000000 INFO @ Sun, 21 Jun 2020 23:37:08: 36000000 INFO @ Sun, 21 Jun 2020 23:37:08: 32000000 INFO @ Sun, 21 Jun 2020 23:37:09: 41000000 INFO @ Sun, 21 Jun 2020 23:37:15: 37000000 INFO @ Sun, 21 Jun 2020 23:37:15: 33000000 INFO @ Sun, 21 Jun 2020 23:37:16: 42000000 INFO @ Sun, 21 Jun 2020 23:37:22: 38000000 INFO @ Sun, 21 Jun 2020 23:37:22: 34000000 INFO @ Sun, 21 Jun 2020 23:37:23: 43000000 INFO @ Sun, 21 Jun 2020 23:37:29: 35000000 INFO @ Sun, 21 Jun 2020 23:37:29: 39000000 INFO @ Sun, 21 Jun 2020 23:37:30: 44000000 INFO @ Sun, 21 Jun 2020 23:37:36: 36000000 INFO @ Sun, 21 Jun 2020 23:37:36: 40000000 INFO @ Sun, 21 Jun 2020 23:37:37: 45000000 INFO @ Sun, 21 Jun 2020 23:37:43: 37000000 INFO @ Sun, 21 Jun 2020 23:37:43: 41000000 INFO @ Sun, 21 Jun 2020 23:37:44: 46000000 INFO @ Sun, 21 Jun 2020 23:37:50: 38000000 INFO @ Sun, 21 Jun 2020 23:37:50: 42000000 INFO @ Sun, 21 Jun 2020 23:37:51: 47000000 INFO @ Sun, 21 Jun 2020 23:37:56: 39000000 INFO @ Sun, 21 Jun 2020 23:37:57: 43000000 INFO @ Sun, 21 Jun 2020 23:37:58: 48000000 INFO @ Sun, 21 Jun 2020 23:38:03: 40000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:38:04: 44000000 INFO @ Sun, 21 Jun 2020 23:38:05: 49000000 INFO @ Sun, 21 Jun 2020 23:38:10: 41000000 INFO @ Sun, 21 Jun 2020 23:38:11: 45000000 INFO @ Sun, 21 Jun 2020 23:38:12: 50000000 INFO @ Sun, 21 Jun 2020 23:38:18: 42000000 INFO @ Sun, 21 Jun 2020 23:38:18: 46000000 INFO @ Sun, 21 Jun 2020 23:38:19: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 23:38:19: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 23:38:19: #1 total tags in treatment: 50780755 INFO @ Sun, 21 Jun 2020 23:38:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:38:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:38:20: #1 tags after filtering in treatment: 50780753 INFO @ Sun, 21 Jun 2020 23:38:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:38:20: #1 finished! INFO @ Sun, 21 Jun 2020 23:38:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:38:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:38:23: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:38:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:38:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:38:25: 43000000 INFO @ Sun, 21 Jun 2020 23:38:26: 47000000 INFO @ Sun, 21 Jun 2020 23:38:32: 44000000 INFO @ Sun, 21 Jun 2020 23:38:33: 48000000 INFO @ Sun, 21 Jun 2020 23:38:39: 45000000 INFO @ Sun, 21 Jun 2020 23:38:41: 49000000 INFO @ Sun, 21 Jun 2020 23:38:47: 46000000 INFO @ Sun, 21 Jun 2020 23:38:48: 50000000 INFO @ Sun, 21 Jun 2020 23:38:54: 47000000 INFO @ Sun, 21 Jun 2020 23:38:54: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 23:38:54: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 23:38:54: #1 total tags in treatment: 50780755 INFO @ Sun, 21 Jun 2020 23:38:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:38:55: #1 tags after filtering in treatment: 50780753 INFO @ Sun, 21 Jun 2020 23:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:38:55: #1 finished! INFO @ Sun, 21 Jun 2020 23:38:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:38:58: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:38:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:38:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:39:00: 48000000 INFO @ Sun, 21 Jun 2020 23:39:07: 49000000 INFO @ Sun, 21 Jun 2020 23:39:14: 50000000 INFO @ Sun, 21 Jun 2020 23:39:19: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 23:39:19: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 23:39:19: #1 total tags in treatment: 50780755 INFO @ Sun, 21 Jun 2020 23:39:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:39:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:39:20: #1 tags after filtering in treatment: 50780753 INFO @ Sun, 21 Jun 2020 23:39:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:39:20: #1 finished! INFO @ Sun, 21 Jun 2020 23:39:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:39:23: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 23:39:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:39:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6827252/SRX6827252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling